Método, kit e iniciadores para a identificação genética de micobacterium bovis bcg cepa moreau-rj, método para monitoramento da viabilidade de bcg moreau-rj e expressão de genes, e, produtos com atividade imunobiológica

Tabela 3 - Mostra a seqüência dos oligonucleotídeos da presente invenção

LISTAGEM DE SEQÜÊNCIAS

1)    INFORMAÇÕES GERAIS:

I.a) Requerente: Fundação Oswaldo Cruz

I.b) Endereço:    Av. Brasil, 4365, Castelo Mourisco, sala 05 e

06, Manguinhos - 21045-900 - Rio de Janeiro - RJ

II)    Titulo da Invenção:    MÉTODO, KIT E INICIADORES PARA A

IDENTIFICAÇÃO GENÉTICA DE MICOBACTERIUM BOVIS BCG CEPA MOREAU-RJ, MÉTODO PARA MONITORAMENTO DA VIABILIDADE DE BCG MOREAU-RJ E EXPRESSÃO DE GENES, E, PRODUTOS COM ATIVIDADE IMUNOBIOLOGICA

III)    Número de Seqüências: 65 (sessenta e cinco)

IV)    Formato para leitura em computador:

IV.a) Meio utilizado: CD-ROM.

IV.b) Computador utilizado: compativel com IBM PC.

IV.c) Sistema operacional: WINDOWS.

2)    INFORMAÇÕES GERAIS DAS SEQÜÊNCIAS:

I. a) Número identificador da seqüência: ID SEQ 1

II)    Características da seqüência:

II. a) tamanho: 36 bp

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II. d) topologia: linear

III)    Posição no genoma:

III. a) posição no mapa: 79594-79594 (inserção)

TCGGCGGTGTGGTGCTTGGGCCTGAGAGATTCTCGGGGAGGAGATTGCTC

CTACGGCGCCTCGACATGGAGGTTCTCCCACATCGCGTCAGCGGCTGTTC

GATTGTGACGGAAAGCAACATACACACCACGCATGTGTTTTGTCACCCTG

CGGTCGCTGGTAGTCGGACGGCCCAATCAGACAGCGCGGGTCATATCACG

CGTTCGTGCACAGTTGGGTGTTTATCCACAGGGGTGCGTTTGTCGGCGGC

TGGCGGGGCGTGGCGGCGATAGCATTCGAATATGAGTTCGATCACGGTGT

CGGTGGACCCGGTGGACCCGGTGGACCCGGTGGACCCGGTGGACCCGGTG

GACCCGGTGGACCCGGTGGACCCGGTGGACCCGGTGGACCCGGTGGACGC

CGTGGTCGCCGCGGGATCAGACGGGCTCACTGTGCCCCGCATCGAGTCCG

AGATCGGGGCCTTGGAGTTCCTGAACGAACTGCGCACTGAACTCAAGAGT

GGACAGTTTCGACCTCAACCGGTGCGGGAACGCAAGATCCCCAAACCGGG

CGGGTTGGGCAAGGTACGGCGGCTGGGGATTCCCACAGTGGCCGACCGGG

TCGTTCAGGCGGCGTTGAAACTGGTGCTAGAACCCATCTTTGAGACCGAC

TTCGAGCCGGTCTCCTACGGGTTTCGGCCCGCGCGACGCGCGCACGACAC

I.a) Número identificador da seqüência: ID SEQ 2

II) Caracteristicas da seqüência:

II.a) tamanho: deleção em M. bovis II.b) tipo: ácido nucléico II.c) conformação da fita: fita senso II.d) topologia: linear

III) Posição no genoma:

III.a) posição no mapa: 79594 - ponto de inserção

atcgc

gtcagcggctgttcgattgtgacggaaagcaacatacac

accacgcatgtgttttgtcaccctgcggtcgctggtagt

cggacggcccaatcagacagcgcgggtcatatcacgcgt

tcgtgcacagttgggtgtttatccacaggggtgcgtttg

tcggcggctggcggggcgtggcggcgatagcattcgaat

accaccccatcggcagattccgtgaagagccagatacgg

tgaaagtcgcacgtccggttcgaagggcggccacgggaa

acggacccgcagcaacgcgggcaccgcacccatggtcga

cccaactgccacgcacccggtgaccggtgcgaagtccac

catatcgaccagtgggcaaccggcggctcaaccgatatc

gacaa

I.a) Número identificador da seqüência: ID SEQ 3

II) Caracteristicas da seqüência:

II.a) tamanho: deleção

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II.d) topologia: linear

III) Posição no genoma:

III.a) posição no mapa: 425072 - 425173 Região contia Moreau:

CCGGCATGCATGCACAGACCCTGCGTACCTACGATCGTCTTGGGTTGGTC

AGCCCGCGGCGCACCTCCGGTGGCGGGCGCCGCTATTCCCTGCATGACGT

CGAGTTGCTGCGCCAGGTGCAGCACCTCTCGCAGGACGAGGGGGTCAACT

TGGCCGGCATCAAGCGCATTATTGAACTGACCAGTCAGGTCGAGGCGCTG

CAGTCCAGGTTGCAAGAGATGGCTGAGGAGTTGGCGGTGTTGCGTGCCAA

CCAGCGCCGCGAGGTCGCGGTGGTGCCGAAGAGCACCGCCCTGGTCGTCT

GGAAACCGCGCCGGTGAGCGAGCGCGCGTAGCGGGGGAGTTAGGGTCCGC

TACCGTTGTTGAGGATGCCGGAGAGTCGGGCTCCGTGGTTGCCGAAGCCG

GAGATAAGGGCTTGGGTCGCGAGGTCCAGCATGCTCGTGTTGTAGAAACC

GGAGACGGTATTGCCTAGGTTCGCCCAGCCCGACAGCAGGTTGCCGAAGT

TTTGGAAGCCCGAATTCCCTACGCCGCCAGCATTGAAGAAGCCCGAAGTC

TCGGTGAAGACGTTTCCCAGGCCCGACACGGCGGCTGCGGCGTCGTTGAG

GAAGCCCGATGCGCCACCGGCGCCGGAGTTGAAGAAGCCCGACGACGGGG

I.a) Número identificador da seqüência: ID SEQ 4

II) Caracteristicas da seqüência:

II.a) tamanho: 102 bp

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II.d) topologia: linear

III) Posição no genoma:

III.a) posição no mapa: 425072-425173

Região M. bovis (Bovilist):

1    -    gtggcgggcg    ccgctattcc    ctgcatgacg    tcgagttgct    gcgccaggtg    cagcacctct

61    -    cgcaggacga    gggggtcaac    ttggccggca    tcaagcgcat    tattgaactg    accagtcagg

121    -    tcgaggcgct    gcagtccagg    ttgcaagaga    tggctgagga    gttggcggtg    ttgcgtgcca

181    -    accagcgccg    cgaggtcgcg    gtggtgccga    agagcaccgc    cctggtcgtc    tggaaaccgc

241    -    gccggtgagc    gagcgcgcgt    agcgggggag    cgaacggcgc    agttggcacc    agccggtgag

301    -    cgagcgcgcg    tagcggggga    gcgaacggcg    cagttggcac    cagccggtga    gcgagcgcgc

361    -    gtagcggggg    agttagggtc    cgctaccgtt    gttgaggatg    ccggagagtc    gggctccgtg

421    -    gttgccgaag    ccggagataa    gggcttgggt    cgcgaggtcc    agcatgctcg    tgttgtagaa

481    -    accggagacg    gtattgccta

I.a) Número identificador da seqüência: ID SEQ 5

II)    Características da seqüência:

II.a) tamanho: deleção

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II. d) topologia: linear

III)    Posição no genoma:

III. a) posição no mapa: 581752-581958

Região contig Moreau

CGGGTTGGCCATCGTCAAACACGTCGCGGCTAATCACGACGGCACCATCC GCGTGTGGAGCAAACÇGGGAACCGGGTCAACGTTCACCTTGGCTCTTCCG GCGTTGATCGAGGCCTATCACGACGACGAGCGACCCGAGCAGGCGCGAGA G C C CG AAC T G C GG T C AAAC AG G T C AC AAC G AG AG G AAG AG C T G AGC C G AT GACCTGCGCCGACGACGATGCAGAGCGTAGCGATGAGGTGGGGGCACCAC CCGCTTGCGGGGGAGAGTGGCGCTGATGACCTGCGCCGACGACGATGCAG AGCGTAGCGATGAGGTGGGGGCACCACCCGCTTGCGGGGGAGAGTGGCGC TGATGACCAGTGTGTTGATTGTGGAGGACGAGGAGTCGCTGGCCGATCCG CTGACGTTTCTGCTGCGCAAGGAGGGCTTTGAGGCCACGGTGGTGACCGA TGGTCCGGCAGCTCTCGCCGAGTTCGACCGGGCCGGCGCCGACATCGTCC TGCTCGATCTGATGCTGCCTGGGATGTCGGGTACCGATGTATGCAAGCAG TTGCGCGCTCGGTCCAGCGTTCCGGTGATCATGGTGACCGCCCGGGATAG CGAGATCGACAAGGTGGTCGGCCTGGAGCTGGGCGCTGACGACTACGTGA

I.a) Número identificador da seqüência: ID SEQ 6

II) Características da seqüência:

II.a) tamanho: 207 pb

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II. d) topologia: linear III) Posição no genoma:

III. a) posição no mapa: 581752-581958

Região M. bovis (Bovilist)

1 -    cgggttggcc    atcgtcaaac    acgtcgcggc    taatcacgac    ggcaccatcc    gcgtgtggag

61- -    caaaccggga    accgggtcaa    cgttcacctt    ggctcttccg    gcgttgatcg    aggcctatca

121 -    cgacgacgag    cgacccgagc    aggcgcgaga    gcccgaactg    cggtcaaaca    ggtcacaacg

181 -    agaggaagag    ctgagccgat    gacctgcgcc    gacgacgatg    cagagcgtag    cgatgaggtg

241 -    ggggcaccac    ccgcttgcgg    gggagagtgg    cgctgatgac    ctgcgccgac    gacgatgcag

301 -    agcgtagcga    tgaggtgggg    gcaccacccg    cttgcggggg    agagtggcgc    tgatgacctg

361 -    cgccgacgac    gatgcagagc    gtagcgatga    ggtgggggca    ccacccgctt    gcgggggaga

421 -    gtggcgctga    tgacctgcgc    cgacgacgat    gcagagcgta    gcgatgaggt    gggggcacca

481 -    cccgcttgcg    ggggagagtg    gcgctgatga    cctgcgccga    cgacgatgca    gagcgtagcg

541 -    atgaggagga    gtggcgctga    tgaccagtgt    gttgattgtg    gaggacgagg    agtcgctggc

601 -    cgatccgctg    gcgtttctgc    tgcgcaagga    gggctttgag    gccacggtgg    tgaccgatgg

661 -    tccggcagct    ctcgccgagt    tcgaccgggc    cggcgccgac    atcgtcctgc    tcgatctgat

721 -    gctgcctggg    atgtcgggta    ccgatgtatg    caagcagttg    cgcgctcggt    ccagcgttcc

781 -    ggtgatcatg    gtgaccgccc    gggatagcga    gatcgacaag    gtggtcggcc    tggagctggg

841 -    cgctgacgac    tacgtgacca    agccctattc    ggcacgcgag    ttgatcgcac    gcatccgcgc

901 -    ggtgctgcgc    cgtggcggcg    acgacgactc    ggagatgagc    gatggcgtgc    tggagtccgg

961 -    gccggttcgc    atggatgtgg    agcgccatgt    cgtctcggtg

I.a) Número identificador da seqüência: ID SEQ 7

II)    Características da seqüência:

II.a) tamanho: deleção

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II. d) topologia: linear

III)    Posição no genoma:

III. a) posição no mapa: 842369-842417

Região contig Moreau

GGCAGCGGCGGGCCCGGCGCCACCGGCCTCGGCGGGATTGGCGGGGCCGG

CGGAGCCGCCTTGCTCTTCGGCTCCGGCGGGGCCGGCGGAAGCGGTGGTG

CCGGCGCGGTCGGTGGCAATGGCGGGGCCGGCGGCAACGCCGGTGCGCTC

TTGGGCGCCGCCGGGGCCGGCGGGGCCGGTGGTGCCGGCGCGGTCGGTGG

CAATGGCGGGGCCGGCGGTAACGGCGGGCTGTTCGCCAACGGGGGAGCCG

GCGGGCCCGGTGGGTTTGGCAGCCCCGCTGGGGCTGGCGGGATCGGCGGG

GCAGGTGGGAACGGCGGGCTGTTCGGCGCCGGCGGGGCGGGCGGCAACGG

CGGGCTGTTCGGCGCCGGCGGCACCGGCGGCGCCGGCAGCCATAGCACCG

CCGCCGGAGTTTCCGGAGGGGCCGGCGGGGCCGGCGGCGACGCCGGCTTG

CTCTCCCTCGGCGCCTCCGGCGGGGCCGGCGGCAGCGGCGGTTCCAGCCT

GACCGCCGCCGGCGTGGTCGGCGGCATCGGCGGCGCCGGAGGCTTGCTCT

TCGGCTCCGGCGGCGCCGGCGGGAGCGGCGGGTTCAGCAACTCTGGCAAC

GGCGGGGCCGGCGGGGCCGGCGGCGACGCGGGTTTGCTCGTCGGCTCCGG

I.a) Número identificador da seqüência: ID SEQ 8

II) Caracteristicas da seqüência:

II.a) tamanho: 48 pb

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II.d) topologia: linear

III) Posição no qenoma:

III.a) posição no mapa: 842369-842417

Região M. bovis (Bovilist):

1 -    gcgggatctt    gttcggcacc    ggcggcaccg    ggggcagcgg    cgggcccggc    gccaccggcc

61 -    tcggcgggat    tggcggggcc    ggcggagccg    ccttgctctt    cggctccggc    ggggccggcg

121 -    gaagcggtgg    tgccggcgcg    gtcggtggca    atggcggggc    cggcggcaac    gccggtgcgc

181 -    tcttgggcgc    cgccggggcc    ggcggggccg    gtggtgccgg    cgcggtcggt    ggcaatggcg

241 -    gggccggcgg    taacggcggg    ctgttcgcca    acgggggagc    cggcgggccc    ggtgggtttg

301 -    gcagccccgc    tggggctggc    gggatcggcg    gggcaggtgg    gaacggcggg    ctgttcggcg

361 -    ccggcgggac    cggcggggcc    ggcgggggaa    gcaccctcgc    cggcggcgcc    ggcggggcgg

421 -    gcggcaacgg    cgggctgttc    ggcgccggcg    gcaccggcgg    cgccggcagc    catagcaccg

481 -    ccgccggagt    ttccggaggg    gccggcgggg    ccggcggcga    cgccggcttg    ctctccctcg

541 -    gcgcctccgg    cggggccggc    ggcagcggcg    gttccagcct    gaccgccgcc    ggcgtggtcg

601 -    gcggcatcgg    cggcgccgga    ggcttgctct    tcggctccgg    cggcgccggc    gggagcggcg

661 -    ggttcagcaa    ctctggcaac    ggcggggccg    gcggggccgg    cggcgacgcg    ggtttgctcg

721 -    tcggctccgg    cggggccggc    ggggccggcg    cctccgccac    cggcgccgcc    accggcgggg

781 -    acggcggggc    cggcggcaag    tccggagcgt    tcggtctcgg    aggtgacggc    ggcgccggcg

841 -    gcgccaccgg    tttgtccggt    gctttccaca    tcggcggcaa    gggcggcgtc    ggcggcagcg

901 -    ccgtgctgat    cggcaacggc    ggcaacggcg    gcaacggcgg    taacagcggt    aacgccggga

961 -    aatccggggg    tgcacccggc    cccagcggcg    ccggcggcgc

I.a) Número identificador da seqüência: ID SEQ 9

II) Caracteristicas da seqüência:

II.a) tamanho: Deleção

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II.d) topoloqia: linear

III) Posição no genoma:

III.a) posição no mapa: 1192598-1192696

Região contiq Moreau

CCGGCACCGGCGCCGCCGCCGCCACCACCCGTACCACCCTTACCGGAAAT

GCCTAGCTGAGTGTCTGCGCCGTTTCCGCCGGCGGCGCCGTTGCCGCCGG

CGCCGCCGTGGCCGGCGTTACCGCCGTGACCGAACAGCGCGGCGCCGCGT

CCGCCGTTGCCGCCGCCGCCAGCGGTGCCGCCGGTGCCGCCGTTGCCGCC

GTTGCCGCCGAAGATGCCGCCCTCTCCGCCGGCACCGGCGTTGCCGCCGC

CGCCGCCGGTGCCGGCGTCGCCGCCGTTGCCGGACAGCCAGCCGGCCGAC

CCGCCGTCGCCGCCGACGCCGCCGTGGCCGTGGATCCAGCCGCCGGCACC

GCCCGCCCCGCCGGCGCCGGCGTCACCGCCCTTGGTGCCGTCGGCCCCGC

CGATGCCGGCGCCGCCTTGTCCGCCGGCCCCGCCGGCACCGCCGGCACCG

CCGTTGCCGAACAATCCGGCCGATCCCCCGGCCCCGCCGGCACCACCCGC

GACGCCCGGCGCGCCGATGGCTCCGGCGGGGCCGGCGCCGCCGGCGCCGC

CATTGCCGCCGCTGCCGTAGAGCCAGCCGCCGTTGCCGCCCGCGCCGCCG

TTGGCGCCGGCTCCGCCGGCCCCTCCGTTGCCGCCGTTGCCGATCAACCC

I.a) Número identificador da seqüência: ID SEQ 10

II)    Características da seqüência:

II.a) tamanho: 99 pb

II.b) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II. d) topologia: linear

III)    Posição no genoma:

III. a) posição no mapa: 1192598-1192696

Região M. bovis (Bovilist)

1 -    ccaccgacgg    ctgtgatgct    gcttccgccg    tttccgctga    ccgcgccgtt    gccgccagcg

61 -    ccgccgtggc    cggcgttacc    gccgtggccg    aacagcacgg    agcctcgtcc    accgttgccg

121 -    ccgttgccgg    cgttgccgcc    gctaccaccg    ttgccaccgt    caccaccttg    tgcggtgcta

181 -    agcccggggg    caccgatccc    gcctttgccg    gcggcgccgc    cgttgccgcc    gttgccgccg

241 -    gccccgccgt    tgccatacag    cagcccgccg    tcgccgccgg    tgccgccggc    accggcgccg

301 -    ccgccgccac    cacccgtacc    acccttaccg    gaaatgccta    gctgagtgtc    tgcgccgttt

361 -    ccgccggcgg    cgccgttgcc    gccggcgccg    ccgtggccgg    cgttaccgcc    gtgaccgaac

421 -    agcgcggcgc    cgcgtccgcc    gttgccgccg    ccgccagcgg    tgccgccggt    gccgccgttg

481 -    ccgccgttgc    cgccgaagat    gccgccctct    ccgccggcac    cggcgttgcc    gccgccgccg

541 -    ccggtgccgg    cgtcgccgcc    gttgccggac    agccagccgg    ccgacccgcc    gtcgccaccg

601 -    cggccgccgg    cqccgcccgc    tccgccqgcq    qcqccggtgt    cgccgggctc    qccctqqacg

661 -    ccgtccccgc    cctgaccgcc    qqttccqccg    tcqccqccqa    cgccgccgtg    gccgtggatc

721 -    cagccgccgg    caccgcccgc    cccgccggcg    ccggcgtcac    cgcccttggt    gccgtcggcc

781 -    ccgccgatgc    cggcgccgcc    ttgtccgccg    gccccgccgg    caccgccggc    accgccgttg

841 - ccgaacaatc cggccgatcc cccggccccg ccggcaccac ccgcgacgcc cggcgcgccg 901 - atggctccgg cggggccggc gccgccggcg ccgccattgc cgccgctgcc gtagagccag 961 - ccgccgttgc cgcccgcgcc gccgttggcg ccggctccgc

I. a) Número identificador da seqüência: ID SEQ 11

II)    Caracteristicas da seqüência:

II. a) tamanho: Deleção

II. b„) tipo: ácido nucléico

II.c) conformação da fita: fita senso

II. d) topologia: linear

III)    Posição no genoma:

III. a) posição no mapa: 1284836-1284907 Região contig Moreau:

GAACCGGTGTCGGCGATTACGGTCAACGTGGTCGGTCCGTCCGGATTGAT GTCCGAACGCGGCCGCAGGGTGGCGCTGCCGGACTTTCCCGTGGTCAGGT T CACCCACGTGACGT TCAGCGGCAACCTCTGCACGTCGGCGGGCCCCGGC GTGCCGACGGCCGTGAACACGTAGGCGGTCTGGCCGGGTCCGGGACCGGG CAGCGGGATCTTGGCGGGCCCCGCCACCGACAGCGCGGTCGCGATGGAAT TGCTGCCGTCCGCCACACAATTGGGGCCGATCGAGGGGTACATGAAGTCC TGTGTGGGCGGAGCGGCGGCGCGCGGCGCAGGTGGCGCCGGTGGCGGCCC CGGTACCGCAACCGGTTGGGCTGCGTCAGGTGCTGGTGCCGGCGCGGAAG CCGGCGGCGCGGCGACCGGCGGCGTAACCGTAGGTGCCACAGCGGGTGCG GGGCCCGCGGCGTGGGATGGATCGATGCCGGTCGGAAGATGTGCCTGCAC ACCTGGCTCGGCGCCCAGTGGGGGCACATGCGGCACCGGGATGGCCTCGG GCAGGGCGACACCATGAGGCGCCGGCACACCCAGGGCAGCAGAGTCGGGA TTGGTCGGCTCAGCCACAAACTGGTTCACCGAGGAAGCGACGTTCTTGGA