Raphael Brum Castro

Áreas de atuação

Participação em eventos

Produções bibliográficas

• Dos Santos, N.A.C. ; Magi, F.N. ; Andrade, A.O. ; Bastos, A.D.S. ; CASTRO, R. B. ; Medeiros, J.F. ; Araújo, M.D.S. . A microbiota de Nyssorhynchus darlingi reduz sua sobrevivência mas não reduz sua suscetibilidade ao Plasmodium vivax. In: I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação, 2021, Porto Velho. Anais do I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação, 2021. v. 1. p. 1-2.

Projetos de pesquisa

• 2018 - 2021

  Investigation of Malaria Vectors (Diptera: Culicidae) in Agricultural Settlements in the Amazon Region of Brazil, Descrição: Environmental changes resulting from the urbanization process represent a challenge for malaria control. The majority of malaria cases in South America occur in rural areas, areas of recent occupation, mining and indigenous areas of the Amazon region. Although these areas have a significant impact on malaria cases, few entomological studies have been carried out in areas of recent occupation. This study aimed to describe the density, natural infection rate and hematophagic behavior of anopheline species in two settlements in the state of Rondonia, Brazil in order to understand how malaria transmission occurs in areas that have been settled at different times.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (4) / Mestrado acadêmico: (2) / Doutorado: (4) . , Integrantes: Raphael Brum Castro - Integrante / Najara Akira Costa dos Santos - Integrante / Alice Oliveira Andrade - Coordenador / Alessandra da Silva Bastos - Integrante / Jansen Fernandes Medeiros - Integrante / Maisa da Silva Araújo - Integrante / Isabele Souza de Araújo - Integrante / Felipe Neves Magi - Integrante / Moreno Magalhães de Souza Rodrigues - Integrante / Dhélio Batista Pereira - Integrante.

• 2018 - 2020

  Description of malaria vectors (Diptera: Culicidae) in two agricultural settlements in the Western Brazilian Amazon, Descrição: The majority of malaria cases in South America occur in rural areas of the Amazon region. Although these areas have a significant impact on malaria cases, few entomological studies have been carried out there. This study aimed to describe entomological parameters in settlements in Rondonia State, Brazil. Collections of anopheles were carried out using the Protected Human Attraction Technique (PHAT). The risk and the potential for malaria transmission were assessed using the human biting rate (HBR), the sporozoite rate (SR) and the entomological inoculation rate (EIR). The results confirmed that Nyssorhynchus darlingi is the predominant species in the two studied locations. Although settlement in the two study sites has occurred at different times, the species richness found was low, showing that environmental changes caused by anthropological actions have probably favor the adaptation of Ny. darlingi species. From the total of 615 anopheline mosquitoes assessed, seven (1.1%) were positive for Plasmodium sp. infections. The EIR revealed that Ny. darlingi contributes to malaria transmission in both locations, as it was responsible for 0.05 infectious bites in humans at night in the old settlement and 0.02 in the recent occupation. In the two study sites, the biting occurred more frequently at dusk. Nyssorhynchus darlingi was prevalent in areas of recent colonization but, even when present in a low density, this species could maintain the transmission of malaria in the older settlement. The entomological information obtained in this study is important and may aid the selection of vector control actions in these locations.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (3) / Mestrado acadêmico: (1) / Doutorado: (5) . , Integrantes: Raphael Brum Castro - Integrante / Najara Akira Costa dos Santos - Integrante / Alice Oliveira Andrade - Coordenador / Alessandra da Silva Bastos - Integrante / Jansen Fernandes Medeiros - Integrante / Maisa da Silva Araújo - Integrante / Isabele Souza de Araújo - Integrante / Felipe Neves Magi - Integrante / Moreno Magalhães de Souza Rodrigues - Integrante / Dhélio Batista Pereira - Integrante.

• 2018 - 2020

  Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil, Descrição: Nyssorhynchus deaneorum is a potential malaria vector because it has been shown to be competent to transmit Plasmodium vivax and Plasmodium falciparum, and because it exhibits antropophilic and endophilic behaviors in some regions of the Amazon. This profile makes Ny. deaneorum a useful mosquito for experiments that model Plasmodium-vector interactions in the Amazon.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (2) / Mestrado acadêmico: (3) / Doutorado: (3) . , Integrantes: Raphael Brum Castro - Integrante / Najara Akira Costa dos Santos - Integrante / Alice Oliveira Andrade - Integrante / Alessandra da Silva Bastos - Integrante / Fábio Resadore - Integrante / Luiz Hidelbrando Pereira da Silva - Integrante / Jansen Fernandes Medeiros - Integrante / Maisa da Silva Araújo - Coordenador.

• 2018 - 2020

  First Observation of Experimental Plasmodium vivax Infection of Three Malaria Vectors from the Brazilian Amazon, Descrição: Although malaria is endemic to the Amazon region, little is known about the susceptibility of potential parasite vectors in Brazil. Assessing the vector susceptibility of Anopheles mosquitoes will increase our understanding of parasite?vector interactions and aid the design of vector control strategies. This study assessed the susceptibility of three Anopheles species to midgut infection by Plasmodium vivax, the predominant malaria species in Rondônia State, Brazil. Blood from P. vivax infected patients was fed to Anopheles aquasalis, Anopheles darlingi, and Anopheles deaneorum mosquitoes using a membrane feeding assay (MFA). Gametocytemia was estimated by microscopic examination of blood smears and oocyst prevalence, and infection intensity was assessed. The presence of oocysts was determined by microscopy, and the infection rates and infection intensity were determined for all species. Data from six MFAs showed that An. darlingi and An. deaneorum exhibited the highest infection rates (97% and 90%, respectively) and developed a similar median number of P. vivax oocysts (142 and 123, respectively), while An. aquasalis exhibited the smallest infection rates (77%) and the median number of oocysts (88). Established laboratory colonies of An. darlingi and An. deaneorum and susceptibility to plasmodial infection would be beneficial for modeling P. vivax vector?parasite interactions in Brazil.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Mestrado acadêmico: (4) / Doutorado: (5) . , Integrantes: Raphael Brum Castro - Integrante / Najara Akira Costa dos Santos - Integrante / Alice Oliveira Andrade - Integrante / Luiz Hidelbrando Pereira da Silva - Integrante / Jansen Fernandes Medeiros - Integrante / Maisa da Silva Araújo - Coordenador / Dhélio Batista Pereira - Integrante / Glaucilene da Silva Costa - Integrante / Antônio Marques Pereira Júnior - Integrante / Luíz Paulo Costa Carvalho - Integrante.