Gabriel Ponciano Carvalho Souza

Formação complementar

Idiomas

Áreas de atuação

Produções bibliográficas

• LIRA, B. S. ; OLIVEIRA, M. J. ; SHIOSE, L. ; VICENTE, MH ; PONCIANO, Gabriel. C. S. ; Floh EIS ; PURGATTO, E ; Nogueira FTS ; Freschi L ; ROSSI, M. . SlBBX28 positively regulates plant growth and flower number in an auxin-mediated manner in tomato. Plant Molecular Biology , 2022.

Projetos de pesquisa

• 2022 - Atual

  Functional characterization of tomato plants edited for SlBBX20, Descrição: BBX proteins encompass a group of zinc-finger transcription factors that contain at least one conserved B-box domain in their N-terminal region. In Arabidopsis thaliana, 32 BBX protein-encoding loci have been identified, which play important roles as regulators of flowering, circadian clock, photo and skotomorphogenesis, biotic and abiotic stress responses, and light signaling transduction. Recently, our research group has identified 31 BBX genes in tomato, Solanum lycopersicum L. Interestingly, mRNA profiling revealed that the plastid type and developmental stage (i.e. proplastid, chloroplast or chromoplast) seem to affect the transcript accumulation pattern of SlBBX genes in leaves, hypocotyls, cotyledons and fruits. Particularly, six genes showed clear association with either early development or ripening of fruits: SlBBX19 (Solyc01g110370), SlBBX20 (Solyc12g089240) and SlBBX26 (Solyc10g006750) were strongly upregulated upon ripening triggering; while, the amount of SlBBX16 (Solyc12g005750), SlBBX28 (Solyc12g005660) and SlBBX29 (Solyc02g079430) mRNA was higher at green stages of fruit development gradually declining afterwards. This expression pattern suggests that these genes may be associated with chloroplast-chromoplast transition, ultimately affecting fruit nutritional quality. Thus, under the hypothesis that proteins containing the B-box domain participate in light signaling regulating tomato vegetative and reproductive development and, eventually, fruit quality, the present work aims to functionally characterize slbbx20 mutant tomato plants produced by CRISPR-Cas9 technology by our research group. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Doutorado: (1) . , Integrantes: Gabriel Ponciano Carvalho Souza - Integrante / Maria Magdalena Rossi - Coordenador / Lumi Shiose - Integrante / Juliene dos Reis Moreira - Integrante., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Auxílio financeiro.

• 2021 - Atual

  Obtainment and genotyping of tomato plants edited for SlBBX genes, Descrição: BBX proteins encompass a group of zinc-finger transcription factors that contain at least one conserved B-box domain in their N-terminal region. In Arabidopsis thaliana, 32 BBX protein- encoding loci have been identified, which play important roles as regulators of flowering, circadian clock, photo and skotomorphogenesis, biotic and abiotic stress responses, and light signaling transduction. Recently, our research group has identified 31 BBX genes in tomato, Solanum lycopersicum L. Interestingly, mRNA profiling revealed that SlBBX genes are regulated by light and six genes showed clear association with either early development or ripening of fruits: SlBBX19 (Solyc01g110370), SlBBX20 (Solyc12g089240) and SlBBX26 (Solyc10g006750) were strongly upregulated upon ripening triggering; while, the amount of SlBBX16 (Solyc12g005750), SlBBX28 (Solyc12g005660) and SlBBX29 (Solyc02g079430) mRNA was higher at green stages of fruit development gradually declining afterwards. This expression pattern suggests that these genes may be associated with chloroplast-chromoplast transition that ultimately might affect fruit nutritional quality. On the other hand, yet unpublished tomato transcriptome data generated by a partner research group demonstrate that the expression of several SlBBX genes is controlled by jasmonic acid (JA). Particularly expression of SlBBX17 and SlBBX20 was significantly increased within an hour of JA treatment and was dependent on JA perception. Several antagonistic molecular mechanisms operating in the crosstalk between JA and light signaling have been described that complicate plants to simultaneously deal with multiple cues, i.e. pathogen and light responses. Thus, improve the knowledge of common players in light and JA signal transduction pathways is highly desired to allow developing uncoupling strategies for breeding or engineering that enable to optimize both responses aiming to improve crop yield and quality. Based on our preliminary data, the BBX proteins emerge as favorite candidates to bridge this link. Thus, under the hypothesis that proteins containing the B-box domain participate in light and JA signaling affecting tomato vegetative and reproductive development and, eventually, fruit quality, the present work aims to obtain tomato plants edited for SlBBX17, SlBBX26, and SlBBX28 genes, for further functional characterization. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Mestrado acadêmico: (1) / Doutorado: (1) . , Integrantes: Gabriel Ponciano Carvalho Souza - Coordenador / Maria Magdalena Rossi - Integrante / Bruno Silvestre Lira - Integrante / Lumi Shiose - Integrante / Maria José de Oliveira - Integrante.

• 2020 - 2021

  Efeito da restrição hídrica no teor de cera foliar e nos componentes das ceras epicuticulares de plantas de Coffea arabica L. recém-germinadas, em condição de vaso, Descrição: O referido trabalho tentou averiguar se plantas de café em estádios iniciais de vida (i.e., logo após a germinação), quando submetidas à restrição hídrica, alteram o teor de cera e a composição metabólica das ceras epicuticulares foliares. Para tal, plântulas da variedade Semperflorens e da cultivar Bourbon Vermelho, que possuem grande valor econômico pelos frutos singulares que produzem, foram sujeitadas a um ciclo de restrição hídrica após o desestiolamento, e foram analisadas por métodos quantitativos, tais como pesagem analítica e cromatografia gasosa acoplada a espectrometria de massas (GCMS). , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) . , Integrantes: Gabriel Ponciano Carvalho Souza - Coordenador / Déborah Yara Alves Cursino dos Santos - Integrante., Financiador(es): Universidade de São Paulo - Bolsa.

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