Washington Luis da Silva

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Produções bibliográficas

• MUTHURAMALINGAM, RAJA ; BARROSO, KAROL ; MILAGRES, JULIANA ; TEDARDI, VITÓRIA ; FRANCO DE OLIVEIRA, FELIPE ; TAKESHITA, VANESSA ; KARMOUS, INES ; EL-TANBOULY, RANIA ; DA SILVA, WASHINGTON . Tiny but Mighty: Nanoscale Materials in Plant Disease Management. PLANT DISEASE , v. 108, p. 241-255, 2024.

• MAIA DA SILVA, ROSECLEIDE ; AMBRÓSIO, MÁRCIA MICHELLE DE QUEIROZ ; NETO, JORGE ALVES DA SILVA ; SILVA, JARLAN LUCAS DOS SANTOS ; DA COSTA, TALISON EUGENIO ; FIGUEIREDO, FRANCISCO ROMÁRIO ANDRADE ; BARROSO, KAROL ALVES ; DA SILVA, WASHINGTON ; HOLANDA, IONÁ SANTOS ARAÚJO . First Report of Lasiodiplodia brasiliensis Causing Root Rot in Melon Plants in Northeastern Brazil. PLANT DISEASE , v. 108, p. 219, 2024.

• THANGAVELU, RAJA MUTHURAMALINGAM ; LUIS DA SILVA, WASHINGTON . Innovative stain-free technique for high-resolution imaging of virus particles via standard transmission electron microscopy. HELIYON , v. 10, p. e26172, 2024.

• BORGES, DARLAN F. ; NOGUEIRA, GEOVANE DE A. ; CRUZ, GISELLYA DE A. ; ARAÚJO, MARIA B. M. ; SILVA, WASHINGTON L. DA ; AMBRÓSIO, MÁRCIA M. DE Q. . Alternative management strategies reduced the incidence and severity of root rot of melon. Revista Caatinga , v. 37, p. e12099, 2024.

• DA SILVA, WASHINGTON L. . Evaluation of fungicides and trichoderma spp. to control soil-borne fungal pathogens on melon crops.. Revista Caatinga , v. 37, p. e12462, 2024.

• LOWRY, GREGORY V. ; GIRALDO, JUAN PABLO ; STEINMETZ, NICOLE F. ; AVELLAN, ASTRID ; DEMIRER, GOZDE S. ; RISTROPH, KURT D. ; WANG, GERALD J. ; HENDREN, CHRISTINE O. ; ALABI, CHRISTOPHER A. ; CAPARCO, ADAM ; DA SILVA, WASHINGTON ; GONZÁLEZ-GAMBOA, IVONNE ; GRIEGER, KHARA D. ; JEON, SU-JI ; KHODAKOVSKAYA, MARIYA V. ; KOHAY, HAGAY ; KUMAR, VIVEK ; MUTHURAMALINGAM, RAJA ; POFFENBARGER, HANNA ; SANTRA, SWADESHMUKUL ; et.al . Towards realizing nano-enabled precision delivery in plants. Nature Nanotechnology , v. 19, p. 1255-1269, 2024.

• MOREIRA, G. V. S. ; TAVARES, M. B. ; CAVALCANTE, A. L. A. ; ALVES, C. P. S. S. ; SALES JUNIOR, R. ; NEGREIROS, A. M. P. ; SOUZA, M. T. Q. ; DA SILVA, WASHINGTON . Reaction of two melon hybrids to Macrophomina phaseolina and M. pseudophaseolina. Revista Caatinga , v. 38, p. e12875-1, 2024.

• ASSUNÇÃO, DANIELA ALMEIDA DE ; EVANGELISTA, LUIZ FERNANDO BEZERRA ; COSTA, TALISON EUGENIO ; SILVA, JARLAN LUCAS SANTOS ; BENTO, ELISANDRA ALVES ; NETO, JORGE ALVES SILVA ; da Silva, Washington Luis ; AMBRÓSIO, MÁRCIA MICHELLE QUEIROZ ; HOLANDA, IONÁ SANTOS ARAÚJO . First Report of Colletotrichum plurivorum and Colletotrichum truncatum Causing Anthracnose on Melon Plants in Brazil. PLANT DISEASE , v. 108, p. 2927, 2024.

• DE ALMEIDA NOGUEIRA, GEOVANE ; COSTA CONRADO, VALÉRIA SAND ; LUIZ DE ALMEIDA FREIRES, AFONSO ; FERREIRA DE SOUZA, JOSÉ JANDERSON ; FIGUEIREDO, FRANCISCO ROMÁRIO ANDRADE ; BARROSO, KAROL ALVES ; MEDEIROS ARAÚJO, MARIA BRUNA ; NASCIMENTO, LUAN VÍTOR ; DE LIMA, JAILMA SUERDA SILVA ; NETO, FRANCISCO BEZERRA ; DA SILVA, WASHINGTON LUÍS ; AMBRÓSIO, MÁRCIA MICHELLE DE QUEIROZ . Aggressivity of Different Fusarium Species Causing Fruit Rot in Melons in Brazil. PLANT DISEASE , v. 107, p. 886-892, 2023.

• BORGES, DARLAN F. ; NOGUEIRA, GEOVANE DE A. ; CRUZ, GISELLYA DE A. ; SILVA, SHAMYRA G. DE A. ; DA SILVA, WASHINGTON L. ; AMBRÓSIO, MÁRCIA M. DE Q. . Changes On Soil Microbiota Induced By The Use Of Commercial Products And The Incorporation Of Plant Materials. Revista Caatinga , v. 36, p. 300-309, 2023.

• GOMES, IZABELLE RODRIGUES FERREIRA ; SILVA, JARLAN LUCAS SANTOS ; COSTA, TALISON EUGÊNIO ; PINTO, PEDRO SIDARQUE LIMA ; da Silva, Washington Luis ; AMBROSIO, MÁRCIA MICHELLE QUEIROZ ; HOLANDA, IONÁ SANTOS ARAÚJO . First Report of Cladosporium tenuissimum causing spot diseases on leaves and fruits of cucurbits in Brazil. PLANT DISEASE , v. 107, p. 2540, 2023.

• KARMOUS, INES ; VAIDYA, SHITAL ; DIMKPA, CHRISTIAN ; ZUVERZA-MENA, NUBIA ; DA SILVA, WASHINGTON ; BARROSO, KAROL ALVES ; MILAGRES, JULIANA ; BHARADWAJ, ANUJA ; ABDELRAHEEM, WAEL ; WHITE, JASON C. ; ELMER, WADE H. . Biologically synthesized zinc and copper oxide nanoparticles using Cannabis sativa L. enhance soybean (Glycine max) defense against fusarium virguliforme. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , v. 194, p. 105486, 2023.

• JAHROMI, AMIRHOSEIN KAZEMIYAN ; ESEHAGHBEYGI, ALI ; SAJADI, S. MOHAMMAD ; NIKBAKHT, ALI ; DA SILVA, WASHINGTON ; SHARIFNABI, BAHRAM ; TOGHRAIE, D. . An application of dielectric barrier discharge treatment to control gray mold growth on cut rose flowers. JOURNAL OF STORED PRODUCTS RESEARCH , v. 104, p. 102170, 2023.

• DUMAS, MADELEINE ; BORGES, DARLAN ; PREISING, STEPHANIE ; TIPPETT, ETHAN ; AMBROSIO, MÁRCIA MICHELLE DE QUEIROZ ; da Silva, Washington Luis . Gathered from the vine: a survey of seven grapevine viruses within New England vineyards. PLANT DISEASE , v. 107, p. 644-650, 2022.

• SHIDORE, TEJA ; ZUVERZA-MENA, NUBIA ; WHITE, JASON C. ; DA SILVA, WASHINGTON . Nanoenabled Delivery of RNA Molecules for Prolonged Antiviral Protection in Crop Plants: A Review. Acs Applied Nano Materials , v. 4, p. 12891-12904, 2021.

• FAROOQ, TAHIR ; ADEEL, MUHAMMAD ; HE, ZIFU ; UMAR, MUHAMMAD ; SHAKOOR, NOMAN ; DA SILVA, WASHINGTON ; ELMER, WADE ; WHITE, JASON C. ; RUI, YUKUI . Nanotechnology and Plant Viruses: An Emerging Disease Management Approach for Resistant Pathogens. ACS Nano , v. 15, p. 6030-6037, 2021.

• PREISING, STEPHANIE ; BORGES, DARLAN FERREIRA ; DE QUEIROZ AMBRÓSIO, MÁRCIA MICHELLE ; DA SILVA, WASHINGTON LUÍS . A Fig Deal: A Global Look at Fig Mosaic Disease and its Putative Associates. Plant Disease , v. 105, p. 727-738, 2021.

• SHIDORE, TEJA ; ZUVERZA-MENA, NUBIA ; DA SILVA, WASHINGTON . Small RNA profiling analysis of two recombinant strains of potato virus Y in infected tobacco plants. VIRUS RESEARCH , v. 288, p. 198125, 2020.

• BORGES, DARLAN FERREIRA ; PREISING, STEPHANIE ; AMBRÓSIO, MÁRCIA MICHELLE DE QUEIROZ ; DA SILVA, WASHINGTON LUÍS . Detection of multiple grapevine viruses in New England vineyards. CROP PROTECTION , v. 132, p. 105143, 2020.

• DA SILVA, WASHINGTON ; KUTNJAK, DENIS ; XU, YI ; XU, YIMIN ; GIOVANNONI, JAMES ; ELENA, SANTIAGO F. ; GRAY, STEWART . Transmission modes affect the population structure of potato virus Y in potato. PLoS Pathogens , v. 16, p. e1008608, 2020.

• DA SILVA, WASHINGTON L. ; YANG, KUEI-TING ; PETTIS, GREGG S. ; SOARES, NATASHA R. ; GIORNO, REBECCA ; CLARK, CHRISTOPHER A. . Flooding-Associated Soft Rot of Sweetpotato Storage Roots Caused by Distinct Clostridium Isolates. PLANT DISEASE , v. 103, p. 3050-3056, 2019.

• ENDELMAN, JEFFREY B ; CARLEY, CARI A SCHMITZ ; BETHKE, PAUL C ; COOMBS, JOSEPH J ; CLOUGH, MARK E ; DA SILVA, WASHINGTON L ; DE JONG, WALTER S ; DOUCHES, DAVID S ; FREDERICK, CURTIS M ; HAYNES, KATHLEEN G ; HOLM, DAVID G ; MILLER, J CREIGHTON ; MUÑOZ, PATRICIO R ; NAVARRO, FELIX M ; NOVY, RICHARD G ; PALTA, JIWAN P ; PORTER, GREGORY A ; RAK, KYLE T ; SATHUVALLI, VIDYASAGAR R ; THOMPSON, ASUNTA L ; et.al . Genetic Variance Partitioning and Genome-Wide Prediction with Allele Dosage Information in Autotetraploid Potato. GENETICS , v. 209, p. 77-87, 2018.

• NASCIMENTO, PAULA G. M. L. ; AMBRÓSIO, MÁRCIA M. Q. ; FREITAS, FRANCISCO C. L. ; CRUZ, BEATRIZ L. S. ; DANTAS, ANDREA M. M. ; JÚNIOR, RUI S. ; DA SILVA, WASHINGTON L. . Incidence of root rot of muskmelon in different soil management practices. EUROPEAN JOURNAL OF PLANT PATHOLOGY , v. 152, p. 433-446, 2018.

• XU, YI ; da Silva, Washington Luis ; QIAN, YAJUAN ; GRAY, STEWART M. . An aromatic amino acid and associated helix in the C-terminus of the potato leafroll virus minor capsid protein regulate systemic infection and symptom expression. PLoS Pathogens , v. 14, p. e1007451, 2018.

• CUI, RUQIANG ; ZHANG, LEI ; CHEN, YUYAN ; HUANG, WENKUN ; FAN, CHENGMING ; WU, QINGSONG ; PENG, DELIANG ; DA SILVA, WASHINGTON ; SUN, XIAOTANG . Expression and evolutionary analyses of three acetylcholinesterase genes ( Mi-ace-1 , Mi-ace-2 , Mi-ace-3 ) in the root-knot nematode Meloidogyne incognita. EXPERIMENTAL PARASITOLOGY , v. 176, p. 75-81, 2017.

• DA SILVA, WASHINGTON L ; INGRAM, JASON ; HACKETT, CHRISTINE A ; COOMBS, JOSEPH J ; DOUCHES, DAVID ; BRYAN, GLENN J ; DE JONG, WALTER ; GRAY, STEWART . Mapping Loci That Control Tuber and Foliar Symptoms Caused by PVY in Autotetraploid Potato ( L.). G3-Genes Genomes Genetics , v. 7, p. 3587-3595, 2017.

• NASCIMENTO, KELLY JULIANE TELLES ; ARAUJO, LEONARDO ; RESENDE, RENATA SOUSA ; SCHURT, DANIEL AUGUSTO ; SILVA, WASHINGTON LUÍS DA ; RODRIGUES, FABRÍCIO DE ÁVILA . Silicon, acibenzolar-S-methyl and potassium phosphite in the control of brown spot in rice. Bragantia (São Paulo) , v. 75, p. 212-221, 2016.

• SILVA, WASHINGTON LUÍS DA ; CRUZ, MARIA FERNANDA ANTUNES ; FORTUNATO, ALESSANDRO ANTÔNIO ; RODRIGUES, FABRÍCIO ÁVILA . Histochemical aspects of wheat resistance to leaf blast mediated by silicon. SCIENTIA AGRICOLA , v. 72, p. 322-327, 2015.

• CLARK, C. C. ; DaSILVA, Washington Luis ; Arancibia, R. A. ; MAIN, J. ; SCHULTHEIS, J. R. ; VAN-ESBROECK, Z. P. ; JIANG, C. ; SMITH, J. . Incidence of End Rots and Internal Necrosis in Sweetpotato is Affected by Cultivar, Curing, and Ethephon Defoliation. Horttechnology , v. 23, p. 886-897, 2013.

• DA SILVA, WASHINGTON L. ; CLARK, CHRISTOPHER A. . Infection of Sweetpotato by and Prior to Harvest. PLANT DISEASE , v. 97, p. 1636-1644, 2013.

• FORTUNATO, ALESSANDRO A. ; da Silva, Washington Luis ; RODRIGUES, FABRICIO . Phenylpropanoid Pathway is Potentiated by Silicon in the Roots of Banana Plants During the Infection Process of Fusarium oxysporum f.sp. cubense. PHYTOPATHOLOGY , v. 104, p. 131218070756001-603, 2013.

• DA SILVA, W. L. ; SINGH, R. . First Report of Causing Leaf Spot on in Louisiana. PLANT DISEASE , v. 96, p. 1379-1379, 2012.

• Sousa-Souto, Leandro ; Schoereder, José H. ; Schaefer, Carlos Ernesto G. R. ; DaSILVA, Washington Luis . Ant nests and soil nutrient availability: the negative impact of fire. Journal of Tropical Ecology , v. 24, p. 639, 2008.

• SCHOEREDER, José Henrique ; Silva, Washington L. . Leaf-Cutting Ants (Hymenoptera: Formicidae) and Soil Classes: Preference, Survival and Nest Density. Sociobiology , v. 52, p. 402-416, 2008.

• Sousa-Sou, Leandro ; GUERRA, Marcelo Braga Bueno ; SCHOEREDER, José Henrique ; SCHAEFER, Carlos Ernesto Gonçalves Reynaud ; Silva, Washington Luis da . Determinação do fator de conversão em colônias de Atta sexdens rubropilosa (Hymenoptera: Formicidae) e sua relação com a qualidade do material vegetal cortado. Revista Árvore (Impresso) , v. 31, p. 163, 2007.

• DaSILVA, Washington Luis ; SCHOEREDER, José Henrique . FORMIGAS SAÚVAS PREFEREM DIFERENTES TIPOS DE SOLOS?. In: I Simpósio Brasileiro de Insetos Sociais, 2005, Belo Horizonte. livro de Anais do evento. Vicosa: UFV, 2005.

• SOUZA, Danival José de ; DaSILVA, Washington Luis ; Sousa-Souto, Leandro . EXISTE DOMINÂNCIA ENTRE RAINHAS EM COLÔNIAS ARTIFICIALMENTE POLIGÍNICAS DE Acromyrmex subterraneus molestans?. In: I Simpósio de Entomologia UFV, 2004, Viçosa. Livro de Anais do Evento. Vicosa: UFV, 2004. v. 18.

• DANIEL, ; FABRICIO, Rodrigues ; DaSILVA, Washington Luis ; FABRICIO, ; WILLER, . Quantificação da atividade enzimática em bainhas de arroz supridas com silício e inoculadas com Rhizoctonia solani. In: XII Congresso Brasileiro de Fisiologia Vegetal, 2009, Rio de Janeiro. Anais do XII Congresso Brasileiro de Fisiologia Vegetal, 2009.

• DaSILVA, Washington Luis ; Rodrigues, F. A. . Histochemical analysis of wheat resistance to leaf blast mediated by silicon. In: APS Southern Division Meetings, 2011, Corpus Christi. 2011 Southern Division Meeting Abstracts, 2011.

• GUERRA, Marcelo Braga Bueno ; Sousa-Souto, Leandro ; SCHOEREDER, José Henrique ; SCHAEFER, Carlos Ernesto Gonçalves Reynaud ; DaSILVA, Washington Luis . PRODUÇÃO DE LIXO EM COLÔNIAS DE ATTA SEXDENS RUBROPILOSA, E SUA RELAÇÃO COM A QUALIDADE DO MATERIAL VEGETAL CORTADO. In: XVII SIMPÓSIO DE MIRMECOLOGIA - Biodiversidade e Bioindicação, 2005, DOURADOS, 2005.

• Sousa-Souto, Leandro ; DaSILVA, Washington Luis ; SCHOEREDER, José Henrique ; SCHAEFER, Carlos Ernesto Gonçalves Reynaud . PAPEL ECOLÓGICO DO FOGO E DA SAÚVA (Atta laevigata) NO RECRUTAMENTO DE RAÍZES EM ÁREA DE CERRADO. In: XVII SIMPÓSIO DE MIRMECOLOGIA - Biodiversidade e Bioindicação, 2005, DOURADOS. Anais do XVII SIMPÓSIO DE MIRMECOLOGIA - Biodiversidade e Bioindicação, 2005.

• GUERRA, Marcelo Braga Bueno ; SCHAEFER, Carlos Ernesto Gonçalves Reynaud ; DaSILVA, Washington Luis ; Sousa-Souto, Leandro ; SCHOEREDER, José Henrique . AVALIAÇÃO DA FERTILIDADE DE SOLOS DE FORMIGUEIROS DE SAÚVAS (Atta sp). In: XIX ENCONTRO REGIONAL DA SBQ-MG, 2005, OURO PRETO, 2005.

• FRANCO OLIVEIRA, FELIPE ; TAKESHITA, VANESSA ; ZUVERZA-MENA, NUBIA ; MILAGRES, JULIANA ; TAMEZ, CARLOS ; da Silva, Washington Luiz ; OLIVEIRA, JHONES LUIZ ; ESPIRITO SANTO PEREIRA, ANDERSON ; FRACETO, LEONARDO FERNANDES ; MARQUES REZENDE, JORGE ALBERTO ; DIMKPA, CHRISTIAN O. ; WHITE, JASON C. . Zein-Based Nanocarrier of the Insecticide Cyantraniliprole for the Control of <i>Bemisia tabaci</i> MEAM1 (Whitefly). Acs Agricultural Science & Technology , 2025.

• SILVA, JARLAN LUCAS SANTOS ; BENTO, ELISANDRA ALVES ; DE MOURA, ANA PAULA ; ALVES, TATIANNE RAIANNE COSTA ; DA SILVA, IGOR VINÍCIUS PEREIRA ; FERNANDES, JULIANO DA COSTA ; DA SILVA FILHO, SILVAN MANOEL ; SOUZA, VITÓRIA MARIA GOMES ; da Silva, Washington Luis ; AMBROSIO, MÁRCIA MICHELLE QUEIROZ . First report of <i>Fusarium falciforme</i> and <i>Fusarium pernambucanum</i> causing root and stem rot on papaya plants in Brazil. PLANT DISEASE , 2025.

• DaSILVA, Washington Luis ; Clark, C. A. . Infection of sweetpotato by fungal end rot pathogens prior to harvest. 2012. (Apresentação de Trabalho/Congresso).

• DaSILVA, Washington Luis ; Clark, C. A. . Infection of sweetpotato by fungal end rot pathogens prior to harvest. 2012. (Apresentação de Trabalho/Congresso).

• DaSILVA, Washington Luis ; Rodrigues, F. A. . Histochemical analysis of wheat resistance to leaf blast mediated by silicon. 2011. (Apresentação de Trabalho/Congresso).

• DaSILVA, Washington Luis . Forgotten Fordlândia: Destruction by a Fungus 2010 (Informative text).

Outras produções

Projetos de pesquisa

• 2022 - Atual

  Using nanoparticles to suppress potato virus Y infection, Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador / AMBRÓSIO, MÁRCIA M. Q. - Integrante., Financiador(es): United States Department of Agriculture - Auxílio financeiro.

• 2022 - Atual

  Tunable release of dsRNA molecules into plants from sustainable nanocarriers: a novel management tool for viral pathogens, Descrição: Plant viruses cause US$30B losses annually worldwide. Viricide for control of viruses in crops is nonexistent and therefore, we rely heavily on the use of viral-resistant plant varieties and prophylactic methods. However, the lack of natural viral-resistant genes in plants coupled with a constant emergence of new virus strains, and the inefficiency of insecticides to control vectorinsects of viruses, make plant virus disease management a challenging task. Thus, there is an increasing demand for innovative and sustainable ways of controlling virus epidemics in agricultural systems. Virus resistance has been induced in plants by the application of exogenous double-stranded RNA (dsRNA), but its effectiveness is short-lived (~ five days). DsRNA is quickly assimilated by plant defense mechanisms and is also degraded by environmental factors. As safe applications of nanomaterials develop, nanotechnology is becoming a promising resource to promote sustainable agriculture. We propose to develop a novel and sustainable nanoparticle platform to deliver an effective and prolonged supply of dsRNAs in plants, with the long-term goal of developing innovative technologies to control plant viruses. We propose to use a staple crop and its most destructive virus pathogen (potato and potato virus Y) as our research system. Our two specific objectives and deliverables are: Short-term: synthesize three different nanoparticle platforms (chitosan, silica, and carbon dots) and test their capacity to provide stable and sustained delivery of dsRNA molecules into potato plant tissue. Long-term: develop a tunable platform for dsRNA delivery (nanoparticle-dsRNA complex) that can be translated to a range of viral crop systems.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (4) / Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador / SHIDORE, TEJA - Integrante / AMBRÓSIO, MÁRCIA M. Q. - Integrante / Nubia Zuverza-Mena - Integrante / Christy Haynes - Integrante / Tania Alvarez - Integrante., Financiador(es): United States Department of Agriculture - Auxílio financeiro.

• 2022 - Atual

  ISOLAMENTO E SELEÇÃO DE BACTÉRIAS EFICAZES NA DEGRADAÇÃO DE ATRAZINA E MANEJO DE FITOPATÓGENOS, Descrição: Bactérias benéficas isoladas do solo podem ser utilizadas como biodegradadoras de herbicidas e também biocontroladoras de patógenos, e a seleção de isolados que tenham potencial tanto para degradar herbicidas do solo, quanto para controlar fitopatógenos é muito útil para agricultura. Assim, o objetivo deste trabalho é o isolamento e seleção de bactérias eficazes na degradação de atrazina e manejo de fitopatógenos. Serão coletadas amostras de diferentes solos que utilizam o herbicida atrazina, e em seguida, isolamentos das bactérias em meio de cultura. Após o isolamento, as bactérias serão cultivadas em meio de cultura contendo atrazina (1000 ppm). Os isolados que apresentarem crescimento neste meio com o herbicida serão purificados e identificados fisiologicamente e molecularmente. As análises fisiológicas serão realizadas no Laboratório de Microbiologia e Fitopatologia da UFERSA, e o sequenciamento na The Connecticut Agricultural Experiment Station (CAES), em New Haven, EUA. Os isolados selecionados por sua capacidade de desenvolver em meio com atrazina serão submetidos a ensaios de biodegradação, sendo as leituras realizadas em HPLC/DAD. Os isolados também serão testados in vitro e in vivo no controle de diferentes patógenos e doenças em cucurbitáceas. Espera-se encontrar isolados de diferentes gêneros de bactérias capazes de degradar no solo o herbicida atrazina, e que estes também possam apresentar eficiência no manejo de patógenos causadores de doenças em plantas... , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Mestrado acadêmico: (1) Doutorado: (3) . , Integrantes: Washington Luis da Silva - Integrante / AMBROSIO, MÁRCIA MICHELLE DE QUEIROZ - Coordenador / Tatianne Raianne Costa Alves - Integrante.

• 2022 - Atual

  ROTAS DE INFECÇÃO E MANEJO ALTERNATIVO DA PODRIDÃO CAUSADA POR Fusarium EM MELÃO, Projeto certificado pela empresa Universidade Federal Rural do Semi-Árido em 18/03/2025., Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Mestrado acadêmico: (1) / Doutorado: (2) . , Integrantes: Washington Luis da Silva - Integrante / AMBRÓSIO, MÁRCIA MICHELLE DE QUEIROZ - Coordenador / Maria Helena Diógenes Costa - Integrante / Jarlan Lucas dos Santos Silva - Integrante.

• 2020 - Atual

  Survey for grapevine leafroll-associated viruses and their vectors in Connecticut for the Implementation of Robust IPM Strategies, Descrição: The state of Connecticut (CT), like much of New England, is home to many family farms that grow a preponderance of fruit crops which are primarily sold directly to consumers at farm stands, pick-your-own, farmers markets, and local grocery stores - where quality is paramount, and any blemishes reduce marketability and farm value. CT is unique in that it is also home to the ever-booming grape wine production. Grape is a high-value crop and extremely susceptible to many pathogens, including viruses. Endemic and emerging virus diseases are becoming a major threat to the grape industry worldwide and preliminary studies have confirmed the presence of the major grapevine viruses in CT and RI vineyards, increasing risk to the sustainability of the state wineries. The overall objective of this study is to determine the extension of the spread and genetic diversity of grapevine viruses in CT vineyards and to develop a statewide management plan that is mechanistically and economically feasible for all growers in the region to adopt.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (3) . , Integrantes: Washington Luis da Silva - Coordenador / AMBRÓSIO, MÁRCIA M. Q. - Integrante., Financiador(es): United States Department of Agriculture - Auxílio financeiro.

• 2020 - Atual

  Nano-enabled technologies: prospective weapons to tackle destructive plant viruses, Descrição: In recent years, virus resistance has been induced in plants by the application of exogenous double-stranded RNA (dsRNA). However, the effectiveness of applying naked dsRNA on leaves is short-lived, dsRNA is quickly assimilated by plant defense mechanisms and is also degraded by environmental factors. We are investigating the potential of using nanoparticles to sustain an effective and prolonged delivery of dsRNA into plants with the long-term goal of developing innovative technologies to control plant virus infections. We have synthesized dsRNAs to target specific regions on the Potato virus Y (PVY) genome, which resulted in 100 protection against PVY on tobacco plants for up to five days. Bioclay nanosheets of ~50nm in diameters were synthetized and dsRNA loaded onto them. The dsRNA-nanoparticle complex was able to provide tobacco plants protection to PVY for up to 20 days, indicating that nanoclays are able to sustainably release dsRNA to protect crops against destructive plant viruses. Current studies are focused on investigating the dsRNA-nanoparticle complex properties to prolong the vaccine effect of slow release dsRNA on plants for direct field application. We are also evaluating other types of nanoparticles, such as Mesoporous Silica and Chitosan, for potential carriers of dsRNAs.. , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Washington Luis da Silva - Coordenador., Financiador(es): American Phytopathological Society - Bolsa.

• 2019 - 2020

  Grant awarded to study fruit crop viruses in Connecticut, Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) . , Integrantes: Washington Luis da Silva - Coordenador / AMBRÓSIO, MÁRCIA M. Q. - Integrante., Financiador(es): CONNECTICUT AGRICULTURAL EXPERIMENT STATION - Bolsa.

• 2018 - 2021

  Using nanoparticles to deliver dsRNA for controlling destructive plant viruses, Descrição: The achievement of virus resistance in plants by the application of exogenous double-stranded RNA (dsRNA) is well documented. Essentially, dsRNA is recruited by the plant RNA silencing machinery to guide the cleavage of complementary virus RNA by a sequence-specific manner, resulting in virus infection suppression. The trouble is that the application of naked dsRNA on plant leaves provides a short-lived protection window (~ five days) against target viruses - it is quickly assimilated by the plant regulatory mechanisms and also degraded by environmental factors. However, just recently nanoparticles have emerged as a promising dsRNA delivering system that protects and gradually delivers dsRNA in plants. Here we propose to establish this technology at CAES with the long-term goal of helping the development of innovative ways to control plant virus infections. Our three specific objectives are: 1) to characterize and screen dsRNAs from tobacco plants infected with potato virus Y (PVY), 2) to evaluate commercially available nanoparticles and to synthesize and characterize nanoparticles as potential dsRNA delivering systems, and 3) to use nanoparticles to sustainably deliver those dsRNA molecules to protect tobacco plants against this devastating virus.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (3) / Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador / AMBRÓSIO, MÁRCIA M. Q. - Integrante / Nubia Zuverza-Mena - Integrante., Financiador(es): CONNECTICUT AGRICULTURAL EXPERIMENT STATION - Auxílio financeiro.

• 2018 - 2021

  Incidence, Prevalence, and Genetic Diversity of Grapevine Viruses in Connecticut, Descrição: Plant diseases are responsible for approximately 40% in crop yield reduction worldwide, resulting in a staggering > $60 billion in crop losses annually - viruses are the second largest group to cause crop yield reduction after fungal pathogens (Savary, Ficke et al. 2012, FAO 2018). Viruses are obligate parasites, need a suitable host to survive and to complete their life cycle, and most of them are transmitted by insect vectors (Hull 2002). Thus, a comprehensive knowledge of the biology of viruses and their vectors as well as their interactions, is crucial to implement effective virus disease control strategies in agricultural systems. The state of Connecticut (CT), like much of New England, is home to many family farms that grow a preponderance of fruit crops which are primarily sold directly to consumers at farm stands, pick-your-own, farmers markets, and local grocery stores - where quality is paramount and any blemishes reduce marketability and farm value. CT is unique in that it is also home to the ever-booming grape wine production. Grape is a high-value crop and extremely susceptible to many pathogens, including viruses. Endemic and emerging virus diseases are becoming a major threat to the grape industry worldwide and amidst the confirmed occurrence of those viruses in neighboring states and the economic importance of the grape industry, no information is available on the occurrence of grapevine virus diseases in CT. The overall objective of this study is to determine the extension of the spread and genetic diversity of grapevine viruses in CT vineyards and to develop a statewide management plan that is mechanistically and economically feasible for all growers in the region to adopt.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (4) . , Integrantes: Washington Luis da Silva - Coordenador / AMBRÓSIO, MÁRCIA M. Q. - Integrante., Financiador(es): United States Department of Agriculture - Auxílio financeiro.

• 2017 - 2017

  American Phytopathological Society Foundation - Myron K. Brakke Travel Award, Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador., Financiador(es): American Phytopathological Society - Bolsa.

• 2016 - 2016

  Mario Einaudi Center International Research Travel Grant, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Washington Luis da Silva - Coordenador., Financiador(es): Cornell University - Bolsa.

• 2016 - 2016

  Cornell Colman Fellowship, Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador., Financiador(es): Cornell University - Bolsa.

• 2016 - 2016

  Cornell University Graduate School Research Travel Grant, Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador., Financiador(es): Cornell College - Bolsa.

• 2014 - 2018

  National Potato Council Scholarship, Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Washington Luis da Silva - Coordenador., Financiador(es): Cornell University - Bolsa.

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