Robert Boehm

Pós-doutorado

Áreas de atuação

Produções bibliográficas

• MOHAMED, S ; BOEHM, Robert ; BINSFELD, Pedro C ; SCHNABL, H . Agrobacterium mediated transformation of two high oleic sunflower (Helianthus annuus L.) genotypes:Assessment and optimization of important parameters. Helia, v. 27, n.40, p. 25-40, 2004.

• ISMAIL, G ; SCHNABL, H ; ZOGLAUER, K ; BOEHM, Robert . Agrobacterium-mediated transformation of Larix decidua: An assessment of factors influencing the efficiency of gus gene transfer. Journal of Applied Botany , v. 78, p. 83-90, 2004.

Projetos de pesquisa

• 2002 - Atual

  Establishment and evaluation of alternative plant expression systems, Descrição: Dr. Robert Boehm worked in the area of plant-derived therapeutics for years. This included enzymatic examinations to plant metabolic pathways for low-molecular weight therapeutics (Boehm et al. 1997), and also their genetic engineering with the aim to increase the production rate (Boehm et al. 2000b). As postdoctoral fellow at the Institute of Agricultural Botany of the University of Bonn, extensive knowledge could be gained in the area of plant culture systems (callus -, suspension - and organcultures), as well as in plant molecular biology (plant transformation and characterization of transgenic plants). These expertises could be applied to the establishment and evaluation of plant expression systems for the production of pharmaceutically relevant proteins, such as tobacco (Boehm et al. 2000a), the duckweed genus of Wolffia (Boehm et al. 2001, Kruse et al. 2002), larch embryogenic cultures (Ismail et al. 2004) and sunflower (Mohamed et al. 2004). Recently the research interest has expanded to the protein glycosylation of recombinant production systems. During a visit at the Institute of Glycobiology, University of Oxford, the glycosylation pattern of recombinant IgE - and IgE-receptor fragments could be elucidated from animal cell cultures and human IgE was purified for further glycosylation studies (manuscript in preparation). The described work field in the border area between medicine, pharmacy and plant biotechnology enables in an ideal manner the successful realization of applied, complex and interdisciplinary projects in the field of therapeutic protein production.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (0) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Robert Boehm - Coordenador.