Cristina Vieira

Pós-doutorado

Idiomas

Orientou

Produções bibliográficas

• MOHAMED, MOURDAS ; DANG, NGUYET THI-MINH ; OGYAMA, YUKI ; BURLET, NELLY ; MUGAT, BRUNO ; BOULESTEIX, MATTHIEU ; MÉREL, VINCENT ; VEBER, PHILIPPE ; SALCES-ORTIZ, JUDIT ; SEVERAC, DANY ; PÉLISSON, ALAIN ; VIEIRA, CRISTINA ; SABOT, FRANÇOIS ; FABLET, MARIE ; CHAMBEYRON, SÉVERINE . A Transposon Story: From TE Content to TE Dynamic Invasion of Drosophila Genomes Using the Single-Molecule Sequencing Technology from Oxford Nanopore. Cells , v. 9, p. 1776, 2020.

• MÉREL, VINCENT ; BOULESTEIX, MATTHIEU ; FABLET, MARIE ; VIEIRA, CRISTINA . Transposable elements in Drosophila. Mobile DNA , v. 11, p. 23, 2020.

• CASTRO, MARCELO R. J. ; GOUBERT, CLÉMENT ; MONTEIRO, FERNANDO A. ; VIEIRA, CRISTINA ; CARARETO, CLAUDIA M. A. . Homology-Free Detection of Transposable Elements Unveils Their Dynamics in Three Ecologically Distinct Rhodnius Species. Genes , v. 11, p. 170, 2020.

• LERAT, EMMANUELLE ; CASACUBERTA, JOSEP ; CHAPARRO, CRISTIAN ; VIEIRA, CRISTINA . On the Importance to Acknowledge Transposable Elements in Epigenomic Analyses. Genes , v. 10, p. 258, 2019.

• FABLET, MARIE ; JACQUET, ANGELO ; REBOLLO, RITA ; HAUDRY, ANNABELLE ; REY, CARINE ; SALCES-ORTIZ, JUDIT ; BAJAD, PRAJAKTA ; BURLET, NELLY ; JANTSCH, MICHAEL F. ; GARCÍA GUERREIRO, MARIA PILAR ; VIEIRA, CRISTINA . Dynamic Interactions Between the Genome and an Endogenous Retrovirus: in Wild-Type Strains. G3-Genes Genomes Genetics , v. 9, p. g3.200789.2018, 2019.

• LERAT, EMMANUELLE ; GOUBERT, CLÉMENT ; GUIRAO'RICO, SARA ; MERENCIANO, MIRIAM ; DUFOUR, ANNE'BÉATRICE ; VIEIRA, CRISTINA ; GONZÁLEZ, JOSEFA . Population-specific dynamics and selection patterns of transposable element insertions in European natural populations. MOLECULAR ECOLOGY , v. 28, p. 1506-1522, 2019.

• GUIO, LAIN ; VIEIRA, CRISTINA ; GONZÁLEZ, JOSEFA . Stress affects the epigenetic marks added by natural transposable element insertions in Drosophila melanogaster. Scientific Reports , v. 8, p. 12197, 2018.

• WALLAU, GABRIEL LUZ ; VIEIRA, CRISTINA ; LORETO, ÉLGION LÚCIO SILVA . Genetic exchange in eukaryotes through horizontal transfer: connected by the mobilome. Mobile DNA , v. 9, p. eCollection 201, 2018.

• GOUBERT, CLEMENT ; HENRI, HELENE ; MINARD, GUILLAUME ; VALIENTE MORO, CLAIRE ; MAVINGUI, PATRICK ; VIEIRA, CRISTINA ; BOULESTEIX, MATTHIEU . High-throughput sequencing of transposable element insertions suggests adaptive evolution of the invasive Asian tiger mosquito towards temperate environments. MOLECULAR ECOLOGY , v. 26, p. 3968-3981, 2017.

• ROMERO-SORIANO, VALÈRIA ; MODOLO, LAURENT ; LOPEZ-MAESTRE, HÉLÈNE ; MUGAT, BRUNO ; PESSIA, EUGÉNIE ; CHAMBEYRON, SÉVERINE ; VIEIRA, CRISTINA ; GARCIA GUERREIRO, MARIA PILAR . Transposable Element Misregulation Is Linked to the Divergence between Parental piRNA Pathways in Drosophila Hybrids. Genome Biology and Evolution , v. 9, p. 1450-1470, 2017.

• LOPEZ-MAESTRE, HÉLÈNE ; CARNELOSSI, ELIAS A. G. ; LACROIX, VINCENT ; BURLET, NELLY ; MUGAT, BRUNO ; CHAMBEYRON, SÉVERINE ; CARARETO, CLAUDIA M. A. ; VIEIRA, CRISTINA . Identification of misexpressed genetic elements in hybrids between Drosophila-related species. Scientific Reports , v. 7, p. 40618, 2017.

• LERAT, EMMANUELLE ; FABLET, MARIE ; MODOLO, LAURENT ; LOPEZ-MAESTRE, HÉLÈNE ; VIEIRA, CRISTINA . TEtools facilitates big data expression analysis of transposable elements and reveals an antagonism between their activity and that of piRNA genes. NUCLEIC ACIDS RESEARCH , v. 45, p. gkw953, 2016.

• GOUBERT, C. ; MODOLO, L. ; VIEIRA, C. ; VALIENTEMORO, C. ; MAVINGUI, P. ; BOULESTEIX, M. . De Novo Assembly and Annotation of the Asian Tiger Mosquito (Aedes albopictus) Repeatome with dnaPipeTE from Raw Genomic Reads and Comparative Analysis with the Yellow Fever Mosquito (Aedes aegypti). Genome Biology and Evolution , v. 7, p. 1192-1205, 2015.

• FABLET, MARIE ; AKKOUCHE, ABDOU ; BRAMAN, VIRGINIE ; VIEIRA, CRISTINA . Variable expression levels detected in the Drosophila effectors of piRNA biogenesis. Gene (Amsterdam) , v. 537, p. 149-153, 2014.

• VELA, DORIS ; FONTDEVILA, ANTONIO ; VIEIRA, CRISTINA ; GARCÍA GUERREIRO, MARÍA PILAR . A Genome-Wide Survey of Genetic Instability by Transposition in Drosophila Hybrids. Plos One , v. 9, p. e88992, 2014.

• BASQUIN, DENIS ; SPIERER, ANNE ; BEGEOT, FLORA ; KORYAKOV, DMITRY E. ; TODESCHINI, ANNE-LAURE ; RONSSERAY, STÉPHANE ; VIEIRA, CRISTINA ; SPIERER, PIERRE ; DELATTRE, MARION . The Drosophila Su(var)3-7 Gene Is Required for Oogenesis and Female Fertility, Genetically Interacts with piwi and aubergine, but Impacts Only Weakly Transposon Silencing. Plos One , v. 9, p. e96802, 2014.

• CARNELOSSI, E. A. G. ; LERAT, E. ; HENRI, H. ; MARTINEZ, S. ; CARARETO, C. M. A. ; VIEIRA, C. . Specific activation of an I-like element in Drosophila interspecific hybrids. Genome Biology and Evolution , v. 6, p. 1806-1817, 2014.

• AKKOUCHE, ABDOU ; GRENTZINGER, THOMAS ; FABLET, MARIE ; ARMENISE, CLAUDIA ; BURLET, NELLY ; BRAMAN, VIRGINIE ; CHAMBEYRON, SÉVERINE ; VIEIRA, CRISTINA . Maternally deposited germline piRNAs silence the tirant retrotransposon in somatic cells. EMBO Reports (Print) , v. 14, p. 458-464, 2013.

• BIÉMONT, C ; VIEIRA, C . Could interallelic interactions be a key to the epigenetic aspects of fitness-trait inbreeding depression?. Heredity , v. 112, p. 219-220, 2013.

• CARARETO, CLAUDIA M.A. ; HERNANDEZ, ERIC H. ; VIEIRA, CRISTINA . Genomic regions harboring insecticide resistance-associated Cyp genes are enriched by transposable element fragments carrying putative transcription factor binding sites in two sibling Drosophila species. Gene (Amsterdam) , v. 537, p. 93-99, 2013.

• AKKOUCHE, A. ; REBOLLO, R. ; BURLET, N. ; ESNAULT, C. ; MARTINEZ, S. ; VIGINIER, B. ; TERZIAN, C. ; VIEIRA, C. ; FABLET, M. . tirant, a Newly Discovered Active Endogenous Retrovirus in Drosophila simulans. Journal of Virology (Print) , v. 86, p. 3675-3681, 2012.

• VIEIRA, CRISTINA ; FABLET, MARIE ; LERAT, EMMANUELLE ; BOULESTEIX, MATTHIEU ; REBOLLO, RITA ; BURLET, NELLY ; AKKOUCHE, ABDOU ; HUBERT, BENJAMIN ; MORTADA, HUSSEIN ; BIÉMONT, CHRISTIAN . A comparative analysis of the amounts and dynamics of transposable elements in natural populations of Drosophila melanogaster and Drosophila simulans. Journal of Environmental Radioactivity , v. 113, p. 83-86, 2012.

• KRAAIJEVELD, KEN ; ZWANENBURG, BRECHTJE ; HUBERT, BENJAMIN ; VIEIRA, CRISTINA ; DE PATER, SYLVIA ; VAN ALPHEN, JACQUES J. M. ; DEN DUNNEN, JOHAN T. ; DE KNIJFF, PETER . Transposon proliferation in an asexual parasitoid. Molecular Ecology (Print) , v. 21, p. 3898-3906, 2012.

• REBOLLO, RITA ; HORARD, BÉATRICE ; BEGEOT, FLORA ; DELATTRE, MARION ; GILSON, ERIC ; VIEIRA, CRISTINA . A Snapshot of Histone Modifications within Transposable Elements in Drosophila Wild Type Strains. Plos One , v. 7, p. e44253, 2012.

• AINOUCHE, ABDELKADER ; BÉTERMIER, MIREILLE ; CHANDLER, MICK ; CORDAUX, RICHARD ; CRISTOFARI, GAËL ; DERAGON, JEAN-MARC ; LESAGE, PASCALE ; QUESNEVILLE, HADI ; PANAUD, OLIVIER ; VAURY, CHANTAL ; VIEIRA, CRISTINA ; VITTE, CLÉMENTINE . International Congress on Transposable Elements (ICTE) 2012 in Saint Malo and the sea of TE stories. Mobile DNA , v. 3, p. 17, 2012.

• LERAT, EMMANUELLE ; BURLET, NELLY ; BIÉMONT, CHRISTIAN ; VIEIRA, CRISTINA . Comparative analysis of transposable elements in the melanogaster subgroup sequenced genomes. Gene (Amsterdam) , v. 473, p. 100-109, 2011.

• GRANZOTTO, ADRIANA ; LOPES, FABRÍCIO R. ; VIEIRA, CRISTINA ; CARARETO, CLAUDIA M. A. . Vertical inheritance and bursts of transposition have shaped the evolution of the BS non-LTR retrotransposon in Drosophila. Molecular Genetics and Genomics , v. 286, p. 57-66, 2011.

• FABLET, MARIE ; VIEIRA, CRISTINA . Evolvability, epigenetics and transposable elements. BioMolecular Concepts , v. 2, p. 333-341, 2011.

• REBOLLO, RITA ; HORARD, BÉATRICE ; HUBERT, BENJAMIN ; VIEIRA, CRISTINA . Jumping genes and epigenetics: Towards new species. Gene (Amsterdam) , v. 454, p. 1-7, 2010.

• MORTADA, HUSSEIN ; VIEIRA, CRISTINA ; LERAT, EMMANUELLE . Genes Devoid of Full-Length Transposable Element Insertions are Involved in Development and in the Regulation of Transcription in Human and Closely Related Species. Journal of Molecular Evolution , v. 71, p. 180-191, 2010.

• FABLET, M. ; LERAT, E. ; REBOLLO, R. ; HORARD, B. ; BURLET, N. ; MARTINEZ, S. ; BRASSET, E. ; GILSON, E. ; VAURY, C. ; VIEIRA, C. . Genomic environment influences the dynamics of the tirant LTR retrotransposon in Drosophila. The FASEB Journal (Online) , v. 23, p. 1482-1489, 2009.

• DELOGER, MARC ; CAVALLI, FLORENCE M.G. ; LERAT, EMMANUELLE ; BIÉMONT, CHRISTIAN ; SAGOT, MARIE-FRANCE ; VIEIRA, CRISTINA . Identification of expressed transposable element insertions in the sequenced genome of Drosophila melanogaster. Gene (Amsterdam) , v. 439, p. 55-62, 2009.

• GRANZOTTO, ADRIANA ; LOPES, FABRÍCIO R ; LERAT, EMMANUELLE ; VIEIRA, CRISTINA ; CARARETO, CLAUDIA MA . The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes. BMC Evolutionary Biology (Online) , v. 9, p. 174, 2009.

• Mugnier N ; Gueguen L ; VIEIRA, C. ; BIEMONT, C. . Heterochromatic copies of the LTR retrotransposons in the Drosophila melanogaster genome. Gene (Amsterdam) , v. 411, p. 87-93, 2008.

• Rebollo R ; Lerat E ; Lopez Kleine L ; BIEMONT, C. ; VIEIRA, C. . Losing helena: the extinction of a drosophila LINE-like element. BMC Genomics , v. 9, p. 149, 2008.

• Fablet M ; Rebollo R ; BIEMONT, C. ; VIEIRA, C. . The evolution of retrotransposon regulatory regions and its consequences on the Drosophila melanogaster and Homo sapiens host genomes. Gene (Amsterdam) , v. 390, p. 88-91, 2007.

• Fablet M ; Souames S ; BIEMONT, C. ; VIEIRA, C. . Evolutionary pathways of the tirant LTR retrotransposon in the Drosophila melanogaster subgroup of species. Journal of Molecular Evolution , v. 64, p. 438-447, 2007.

• Fablet M ; McDonald JF ; BIEMONT, C. ; VIEIRA, C. . Ongoing loss of the tirant transposable element in natural populations of D. simulans. Gene (Amsterdam) , v. 375, p. 54-62, 2006.

• BIEMONT, C. ; VIEIRA, C. . Genetics: Junk DNA as an evolutionary force. Nature (London) , v. 443, p. 521-524, 2006.

• BIEMONT, C. ; VIEIRA, C. . What Transposable elements tell us about genome organisation and evolution ? The case of Drosophila. Cytogenetic and Genome Research , v. 110, p. 25-34, 2005.

• Mugnier N ; BIEMONT, C. ; VIEIRA, C. . New regulatory regions of drosophila 412 retrotransposable element generated by recombination. Molecular Biology and Evolution , v. 22, p. 747-757, 2005.

• NARDON, C ; DECELIERE, G ; LOEVENBRUCK C ; M, W. ; VIEIRA, C. ; BIEMONT, C. . Is genome size influenced by colonization of new environments in Dipteran species ?. Molecular Ecology , v. 14, p. 869-878, 2005.

• VIEIRA, C. ; BIEMONT, C. . Transposable Element Dynamics in Two Sibling Species: Drosophila melanogaster and Drosophila simulans. Genetica (The Hague) , v. 120, p. 115-123, 2004.

• BIEMONT, C. ; NARDON, C ; DECELIERE, G ; LEPETIT, D ; LOEVENBRUCK C ; VIEIRA, C. . Worldwide distribution of transposable elements copy number in natural populations of Drosophila simulans. Evolution (Lancaster) , v. 57, p. 159-167, 2003.

• NARDON, C ; M, W. ; VIEIRA, C. ; BIEMONT, C. . Variation in genome size estimate with environmental conditions in Drosophila melanogaster. Cytometry. Part A , v. 55, p. 43-9, 2003.

• VIEIRA, C. ; NARDON, C ; ARPIN, C ; JUNAKOVIC, N ; LEPETIT, D ; BIEMONT, C. . Evolution of genome size in Drosophila. Is the invaders genome being invaded by transposable elements?. Molecular Biology and Evolution , v. 19, p. 1154-1161, 2002.

• BIEMONT, C. ; VIEIRA, C. ; BORIE, N . Éléments transposables et évolution du génome dune espèce invasive : le cas de Drosophila simulans. Genetics Selection Evolution , v. 33, p. S107-S120, 2001.

• BIEMONT, C. ; VIEIRA, C. ; BORIE, N ; LEPETIT, D . Transposable elements and genome evolution : the case of D.simulans. Genetica (The Hague) , v. 107, p. 113-120, 2000.

• VIEIRA, C. ; PIGANEAU, G ; BIEMONT, C. . Mobilisation of various transposable elements in an australian population of D. simulans. Genetical Research , v. 76, p. 117-119, 2000.

• PASYUKOVA, EG ; VIEIRA, C. ; MACKAY, TFC . Deficiency mapping of quantitative trait loci affecting longevity in Drosophila melanogaster. Genetics (Austin) , v. 156, p. 1129-1140, 2000.

• VIEIRA, C. ; PASYUKOVA, EG ; ZENG, ZB ; HACKETT, J. B ; LYMAN, RF ; MACKAY, TFC . Genotype-Environment interaction for quantitative trait loci affecting lifespan in Drosophila melanogaster. Genetics (Austin) , v. 154, p. 213-227, 2000.

• VIEIRA, C. ; LEPETIT, D ; DUMONT, S ; BIEMONT, C. . Wake of transposable elements following D. simulansworldwide colonization. Molecular Biology and Evolution , v. 16, p. 1251-1255, 1999.

• JUNAKOVIC, N ; TERRINONI, A. ; DI FRANCO, C ; VIEIRA, C. ; LOEVENBRUCK C . Accumulation of transposable elements in the heterochromatin and on the Y chromosome of Drosophila simulans and D. melanogaster. Journal of Molecular Evolution , v. 46, p. 661-668, 1998.

• VIEIRA, C. ; AUBRY, P ; LEPETIT, D ; BIEMONT, C. . A temperature cline in copy number for 412 but not roo/B104 retrotransposons in populations of Drosophila simulans. Proceedings of the Royal Society of London. Biological Sciences , v. 265, p. 1161-1165, 1998.

• VIEIRA, C. ; BIEMONT, C. . Transposition rate of the 412 retrotransposable element is independent of copy number in natural populations of Drosophila simulans. Molecular Biology and Evolution , v. 14, p. 185-188, 1997.

• HOOGLAND C ; VIEIRA, C. ; BIEMONT, C. . Chromosomal distribution of the 412 retrotransposon in natural populations of Drosophila simulans. Heredity (Edinburgh) , v. 79, p. 128-134, 1997.

• BIEMONT, C. ; VIEIRA, C. ; HOOGLAND C ; CIZERON G ; LOEVENBRUCK C ; ARNAULT C ; CARANTE, J P . Maintenance of transposable element copy number in natural populations of Drosophila melanogaster and D. simulans. Genetica (The Hague) , v. 100, p. 161-166, 1997.

• BIEMONT, C. ; TSITRONE, A ; VIEIRA, C. ; HOOGLAND C . Transposable element distribution in Drosophila. Genetics (Austin) , v. 147, p. 1997-1999, 1997.

• VIEIRA, C. ; BIEMONT, C. . Geographical variation in insertion site number of retrotransposon 412 in Drosophila simulans. Journal of Molecular Evolution , v. 42, p. 443-451, 1996.

• VIEIRA, C. ; BIEMONT, C. . Selection against transposable element insertions in Drosophila simulans and Drosophila melanogaster. Genetical Research , v. 68, p. 9-15, 1996.

• VIEIRA, CRISTINA ; FABLET, MARIE ; LERAT, EMMANUELLE . Infra- and Transspecific Clues to Understanding the Dynamics of Transposable Elements. Genome Dynamics and Stability. 1ed.: Springer Berlin Heidelberg, 2009, v. , p. 21-43.

• ROMERO-SORIANO, VALÈRIA ; BURLET, NELLY ; VELA, DORIS ; FONTDEVILA, ANTONIO ; VIEIRA, CRISTINA ; GARCIA GUERREIRO, MARIA PILAR . <i>Drosophila</i> females undergo genome expansion after interspecific hybridization. Genome Biology and Evolution , 2016.

Projetos de pesquisa

• 2015 - Atual

  The genetic basis of adaptation to new environments: the Asian tiger mosquito case, Descrição: Aedes albopictus (the Asian tiger mosquito) is an invasive species. From its cradle in Asia it has colonized Europe, America and Africa during the last 30 years, mainly through intercontinental trade in used automobile tires where females lay their eggs. In France its range at first limited to the South East is rapidly expanding. The goal of our project is to understand if the success of the colonizing populations of A. albopictus is due to particular genetic characteristics that distinguishes them from the Asian populations. To answer this question we are comparing Asian and recently established populations by genotyping individuals at thousands of markers, TEs-based or not, distributed throughout the genome, taking advantage of the most recently developed sequencing technologies.. , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Cristina Vieira - Coordenador / Matthieu Boulesteix - Integrante / Clément Goubert - Integrante / Claire Valiente-Moro - Integrante / Patrick Mavingui - Integrante / Frédéric Simard - Integrante / Didier Fontenille - Integrante / Grégory LAmbert - Integrante.

• 2014 - Atual

  The effect of range expansions on TE content, Descrição: Range expansions of populations/species may be accompanied by a series of founder events. Such founder events imply an intense genetic drift on the lip of an expanding population. In this situation we expect natural selection to be less effective at controlling TEs proliferation. We investigate this idea by means of modelling, experimental evolution and in natura assessment.. , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Cristina Vieira - Coordenador / Matthieu Boulesteix - Integrante.

• 2014 - Atual

  Transposable elements and hybrids incompatibility, Descrição: genet_inc.png
	It has been postulated in the literature that TE may be some how implicated in hybrid incompatibility, or that they are associated with speciation events. At the intra species level, this was typically shown in the case of the hybrid digenesis in Drosophila, in which several different TEs may be involved. We are addressing this question with two different models. One consists in the analysis of hybrids from D. mojavensis and D. arizonae. This two species diverged less the 1 Mya and it is still possible to produce hybrids in the laboratory. We are analyzing the dynamics of TEs in the hybrids versus the parental lines. The other deals with a lower level of divergence using intra-specific hybrids between distinct natural populations of D. melanogaster or D. simulans. This projet is funded by the ANR ExHyb. , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Cristina Vieira - Coordenador / CARARETO, CLAUDIA M. A. - Integrante / Vincent Lacroix - Integrante / Helene Lopez - Integrante / Marie Fablet - Integrante., Financiador(es): Agence Nationale de la Recherche - Auxílio financeiro.

• 2012 - Atual

  Transposable element dynamics and variability between and within species, Descrição: Transposable elements are found in the genomes of virtually all living organisms, but their proportions and activities vary greatly between and within species. In order to understand TE dynamics, we focus on wild-type strains of Drosophila melanogaster and D. simulans, two closely related species which have a three-fold difference in their TE content. In particular, we explore variability in epigenetic TE regulation in relationship to variability in TE dynamics and content. One of the major defense mechanisms against TEs in Drosophila is an RNA interference machinery based on a particular class of small RNAs: piwi-interacting small RNAs (piRNAs). We are currently investigating the link between differences in TE and piRNA content between different populations and species of Drosophila using available genomes and NGS data. We focus on tirant, an endogenous retrovirus which activity varies between wild-type strains of D.simulans: while tirant is either inactive and embedded in heterochromatin in most strains, or it displays post-transcriptionaly silenced euchromatic copies, we identified one strain in which tirant behaves as an endogenous retrovirus and increases in copy number. We use this system of three-type wild-type strains to decipher the dynamics of tirant, with an emphasis on the regulation by piRNAs.. , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Cristina Vieira - Coordenador / Marie Fablet - Integrante / Laurent Modolo - Integrante / Abdou Akkouche - Integrante / Séverine Chambeyron - Integrante / Christophe Terzian - Integrante.

• 2007 - 2012

  Análise da dinâmica de aquisição e perda de elementos transponíveis no genoma: o elemento Helena de Drosophila como modelo, Descrição: O elemento transponível Helena propicia uma oportunidade única para compreender a dinâmica de aquisição e perda de elementos em um genoma, já que em D. melanogaster há uma provável inativação desse retroposon e em D. simulans, uma atividade potencial do elemento. Além disso Drosophila é um excelente modelo de estudo, pois a quantidade de elementos é suficientemente pequena para permitir estudos exaustivos e completos. Para compreender o processo dinâmico de aquisição e perda de Helena nos genomas, este trabalho tem os objetivos de analisar a ocorrência do elemento Helena, in silico e em populações naturais, de 12 espécies de Drosophila (D. melanogaster, D. simulans, D. yakuba, D. erecta, D. sechellia, D. ananassae, D. persimilis, D. pseudoobscura, D. virilis, D. mojavensis, D. grimshawi, D. willistoni). As análises de Bionformática permitirão descrever o número de inserções de Helena no genomas, a diversidade nucleotidica, as relações evolutivas. e: o grau de deterioração entre cópias da mesma espécie e entre espécies. As análises populacionais de D. simulans e de D. melanogaster, como também de espécies subgrupo melanogaster e de D. virilis por meio de Southern blot permitirá detectar possíveis cópias completas e analisar sua expressão por meio de RT-PCR e Northern blot. Com esse estudo, sobretudo pelas análises populacionais em espécies irmãs (D. melanogaster e D. simulans), mas que apresentam dinâmica diferente de evolução do retroposon Helena, poderemos compreender como as modificações da estrutura e a composição desse elemento, um fenômeno que ocorre em etapas e velocidades variáveis em diferentes espécies. Com este tipo de análise comparativa poderemos compreender como o elemento Helena, em particular, e os retroposons em geral, são adquiridos e perdidos pelas espécies e como características, tais como os sítios de inserção, o genoma hospedeiro e o tempo de integração das cópias completas no genoma hospedeiro relacionam-se com a sua dinâmica evolutiva.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Cristina Vieira - Integrante / GRANZOTTO, ADRIANA - Integrante / CARARETO, CLAUDIA M. A. - Coordenador / Emmanuelle Lerat - Integrante., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Auxílio financeiro / Fundação de Amparo à Pesquisa do Estado de São Paulo - Bolsa / Centre National de la Recherche Scientifique - Auxílio financeiro / Agence Nationale de la Recherche - Auxílio financeiro / Institute Universitaire de France - Auxílio financeiro / Conselho Nacional de Desenvolvimento Científico e Tecnológico - Bolsa.