Oliver Holub

Pós-doutorado

Idiomas

Áreas de atuação

Participação em eventos

Participação em bancas

Produções bibliográficas

• WASILEWSKA-SAMPAIO, Ana Paula ; SILVEIRA, Mariana S. ; HOLUB, Oliver ; GOECKING, Rejane ; GOMES, Flávia C A ; MOURA NETO, Vivaldo ; LINDEN, Rafael ; FERREIRA, Sérgio T. ; FELICE, Fernanda G. de . Neuritogenesis and neuronal differentiation promoted by 2,4-dinitrophenol, a novel anti-amyloidogenic compound. The FASEB Journal , Bethesda, MD, USA, v. 19, p. 1627-1636, 2005.

• HOLUB, Oliver ; SEUFFERHELD, Manfredo J ; GOHLKE, Christoph ; GOVINDJEE ; CLEGG, Robert M . Fluorescence lifetime imaging (FLI) in real-time - a new technique in photosynthesis research. Photosynthetica , Prague, v. 38, n.4, p. 581-599, 2000.

• CLEGG, Robert M ; HOLUB, Oliver ; GOHLKE, Christoph . Fluorescence Lifetime-resolved Imaging. Measuring lifetimes in an image. Why do it? How to do it. How to interpret it. In: Gerard Marriot; Ian Parker. (Org.). Methods in Enzymology; Biophotonics; Part A (two-volume issue). San Diego: Academic Press, 2003, v. 360, p. 509-542.

• TRAMIER, Marc ; HOLUB, Oliver ; CRONEY, John C ; ISHII, Takahisa ; SEIFRIED, Steven E. ; JAMESON, David M. . Binding of ethidium to yeast tRNAPhe : A new perspective on an old Bromide. In: Kraayenhof, R.; Visser, A.J.W.G.; Gerritsen, H.C.. (Org.). Fluorescence Spectroscopy, Imaging and Probes. Berlin, Heidelberg, New York: Springer-Verlag, 2001, v. , p. 111-121.

• MORAIS DA SILVA, Ana Christina ; FERREIRA, Sérgio T. ; HOLUB, Oliver . Microglial phagocytosis of giant aggregates of b-amyloid. . In: SBBq - XXXIV Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular (Annual Meeting of the Brazilian Society of Biochemistry & Molecular Biology), Hotel Monte Real, Águas de Lindóia, São Paulo, July 02-05, 2005, 2005, Águas de Lindóia, 2005.

• HOLUB, Oliver . Fluorescence lifetime imaging at video rate. In: Palestra:, 2005, Riberão Preto. Palestra: Faculdade de Filosofia, Ciências e Letras de Riberão Preto da Universidade de São Paulo, Riberão Preto, SP, Brazil, May 30, 2005., 2005.

• HOLUB, Oliver . Shining light on Alzheimer's Disease: Using light scattering to study synthetic amyloid plaques. . In: Palestra:, 2005, Riberão Preto. Invited talk by the Faculdade de Filosofia, Ciências e Letras de Riberão Preto da Universidade de São Paulo, Lecture series of Profa. Dra. Catarina Satie Takahashi, Anfiteatro Prof. Lucien Lison, Riberão Preto, SP, Brazil, May 31, 2005., 2005.

• HOLUB, Oliver ; FERREIRA, Sérgio T. . Development of a routine for the quantitative analysis of fluorescence microscopy images. In: SBBq - Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular (Annual Meeting of the Brazilian Society of Biochemistry & Molecular Biology), Hotel Glória, Caxambu, MG, Brazil, May 15-18, 2004, 2004, Caxambu, 2004.

• HOLUB, Oliver ; PAULA-LIMA, Andrea C. ; LOUZADA, Paulo R. ; FERREIRA, Sérgio T. . Stability of honeycomb beta-amyloid aggregates investigated by light microscopy and light scattering in comparison with general Mie scattering theory. In: SBBq - Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular (Annual Meeting of the Brazilian Society of Biochemistry & Molecular Biology), Hotel Glória, Caxambu, MG, Brazil, May 15-18, 2004, 2004, Caxambu, 2004.

• WASILEWSKA-SAMPAIO, Ana Paula ; GOECKING, Rejane ; SILVEIRA, Mariana S. ; HOLUB, Oliver ; GOMES, Flávia A. ; MOURA-NETO, Vivaldo ; LINDEN, Rafael ; FELICE, Fernanda G. de ; FERREIRA, Sérgio T. . Neuritogenesis and cell differentiation induced by 2,4-Dinitrophenol, a novel anti-amyloidogenic compound. In: SBBq - Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular (Annual Meeting of the Brazilian Society of Biochemistry & Molecular Biology), Hotel Glória, Caxambu, MG, Brazil, May 15-18, 2004, 2004, Caxambu, 2004.

• HOLUB, Oliver . Fluorescence lifetime imaging at video rate. In: XIX Reunião Anual da FeSBE, 2004, Águas de Lindóia/S.Paulo. Palestra; Simpósio: Fluorescência no Estudo de Sistemas Biológicos, 2004.

• HOLUB, Oliver ; SEUFFERHELD, Manfredo J ; GOHLKE, Christoph ; GOVINDJEE ; CLEGG, Robert M . Fluorescence lifetime imaging at video rate - applications in photosynthesis. In: Annual Photosynthesis Conference & Retreat, Robert Allerton House, Monticello, IL, March 3-4, 2001, 2001, Monticello. Oral presentation, 2001.

• CLEGG, Robert M ; HOLUB, Oliver ; GOHLKE, Christoph ; BREUSEGEM, Sophia ; BARRY, Nicholas ; GRATTON, Enrico . Real-time fluorescence lifetime-resolved imaging - why we do it, how it's done and, applications for biology and medicine. In: CYTO 2001 FLIM, FRET and Multiphoton Microscopy; St Catherine's College, University of Oxford, July 1-3, 2001, 2001, Oxford. Oral Presentation, 2001.

• GOVINDJEE ; HOLUB, Oliver ; SEUFFERHELD, Manfredo J ; GOHLKE, Christoph ; CLEGG, Robert M . Fluorescence lifetime imaging microscopy (FLIM) of non-photochemical quenching mutants of C. reinhardtii. In: Light stress and photosynthesis UV-B and visible light effects (a satellite meeting for PS2001, The 12th International Congress on Photosynthesis); Heron Island Resort, Queensland, Australia, August 13-17, 2001, 2001, Heron Island Resort. NPQ Planary Lecture; Oral presentation, 2001.

• HOLUB, Oliver ; SEUFFERHELD, Manfredo J ; GOHLKE, Christoph ; CLEGG, Robert M ; GOVINDJEE . Real-time fluorescence lifetime-resolved images of individual cells of wild type and NPQ mutants of Chlamydomonas reinhardtii. In: 9th International Conference on the Cell and Molecular Biology of Chlamydamonas, Amsterdam, The Netherlands, May 21-26, 2000, 2000, Amsterdam, 2000.

• GOHLKE, Christoph ; HOLUB, Oliver . FlimFast - Fluorescence Lifetime Imaging at Video Rate: Demonstration of continuous fluorescence lifetime image measurements with concurrent analysis and visualization at 3-26 Hz. In: Movies for download at http://lfd.uiuc.edu/staff/gohlke/flimfast(+1-3).wmv, 2000. Movies for download.

• HOLUB, Oliver ; CLEGG, Robert M . Stereochemistry of DNA molecules by homo-energy transfer. In: 12th Annual Gibbs Conference on Biothermodynamics, Carbondale, Illinois, October 3-6, 1998, 1998, Carbondale, 1998.

• HOLUB, Oliver ; CLEGG, Robert M . Stereochemistry of DNA molecules by homo-energy transfer. In: Second Conference on Fluorescence Microscopy & Fluorescent Probes, Prague, Czechia, April 9-12, 1997, 1997, Prague, 1997.

• SCHNEIDER, Peter C ; HOLUB, Oliver ; CLEGG, Robert M . Real-Time Fluorescence Lifetime-Resolved Imaging. In: Second Conference on Fluorescence Microscopy & Fluorescent Probes, Prague, Czechia, April 9-12, 1997, 1997, Prague, 1997.

• VANDEVEN, Martin J. ; HOLUB, Oliver ; GOHLKE, Christoph ; GOVINDJEE ; VALCKE, Roland ; AMELOOT, Marcel ; CLEGG, Robert M . Fast, Macroscopic Chlorophyll Fluorescence Lifetime Imaging of Apple Fruit Skin. Biophysical Journal , San Francisco, v. 82, n.1, p. ???a-???a, 2002.

• GOHLKE, Christoph ; HOLUB, Oliver ; CLEGG, Robert M . FlimFast - Fluorescence Lifetime Image Acquisition and Visualization at Video-Rate. Biophysical Journal , Boston, v. 80, n.1, p. 169a-169a, 2001.

• HOLUB, Oliver ; SEUFFERHELD, Manfredo J ; GOHLKE, Christoph ; GOVINDJEE ; CLEGG, Robert M . Application of real-time fluorescence lifetime-resolved imaging in photosynthesis: Studies of maize leaves (Zea Mays), small mustard leaves (Arabidopsis thaliana), and of individual wild-type and mutant cells of the green alga Chlamydomonas reinhardtii. Biophysical Journal , Boston, v. 80, n.1, p. 428a-428a, 2001.

• HOLUB, Oliver ; GOHLKE, Christoph ; SEUFFERHELD, Manfredo J ; GOVINDJEE ; CLEGG, Robert M . Want to know something about your fluorescent samples that optics cannot resolve? Long working-distance stage-scanning instrument for Real-Time Fluorescence Lifetime-Resolved Imaging. Biophysical Journal , New Orleans, v. 78, n.1, p. 248A-248A, 2000.

• TRAMIER, Marc ; HOLUB, Oliver ; CRONEY, John ; ISHII, Takahisa ; SEIFRIED, Steven E. ; JAMESON, David M. . New perspectives on an old bromide: Ethidium binding to yeast tRNAPhe. Biophysical Journal , New Orleans, v. 78, n.1, p. 305A-305A, 2000.

• HOLUB, Oliver ; CLEGG, Robert M . Second order sequence specificity of DAPI-binding to AT-regions in DNA. Biophysical Journal , Baltimore, v. 76, n.1, p. A129-A129, 1999.

• HOLUB, Oliver ; CLEGG, Robert M . Stereochemistry of DNA molecules by homo-energy transfer. Biophysical Journal , Kansas City, v. 74, n.2, p. A288-A288, 1998.

• SCHNEIDER, Peter C ; HOLUB, Oliver ; CLEGG, Robert M . Real-Time Fluorescence Lifetime-Resolved Images of 5-Aminolevulinic Acid-Induced Protoporphyrin IX in Cells. Biophysical Journal , Kansas City, v. 74, n.2, p. A189-A189, 1998.

Outras produções

Projetos de pesquisa

• 2005 - Atual

  Setup of a synchrotron fluorometer, Descrição: A synchrotron-radiation frequency-domain fluorometer for time-resolved fluorescence measurements has been installed at the Brazilian Synchrotron Light Laboratory (LNLS) in Campinas. The commissioning is ongoing during several visits at the synchrotron. . , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (0) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Oliver Holub - Coordenador.

• 2003 - Atual

  Investigation of giant aggregates of beta-amyloid, Descrição: Stability of honeycomb beta-amyloid aggregates investigated by light microscopy and light scattering in comparison with general Mie scattering theory. In aqueous medium, the beta-amyloid peptide (Abeta) partly exists as large (ca. 100 micro-m) aggregates, which, when examined under light microscopy, resemble a honeycomb structure with repetitive elements of about 3 micro-m. In treatments of neuronal cultures with Abeta, these structures exhibit a remarkable longevity. Direct microscopic observation reveals a significant stability even in the presence of denaturating agents such as urea and guanidine hydrochloride. Right-angle light scattering experiments in the presence of these chaotropic agents seem to corroborate these results, if the changes in refractive index of the medium in the presence of denaturants are taken into account. The experimental results are further compared with theoretical results obtained by calculations using general Mie scattering theory. . , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (0) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Oliver Holub - Coordenador / Andrea C. Paula Lima - Integrante / Sérgio T. Ferreira - Integrante., Financiador(es): Conselho Nacional de Desenvolvimento Científico e Tecnológico - Bolsa.

Projetos de desenvolvimento

• 2005 - Atual

  Setup of a two-photon fluorescence microscope, Descrição: A grant application for the purchase of a two-photon microscope was filed by Prof. Rafael Linden from the Instituto de Biofísica Carlos Chagas Filho and our institut (Instituto de Bioquímica Médica) and has been approved. I am involved in the tecnical negociations for the purchase.. , Situação: Em andamento; Natureza: Desenvolvimento. , Alunos envolvidos: Graduação: (0) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Oliver Holub - Coordenador.

• 2003 - 2004

  Fluorescence microscopy image analysis, Descrição: A routine for histogram analysis of images has been written in the object-oriented, graphical development environment LabVIEW. The program converts an RGB bitmap image into an intensity-linear grayscale image according to selected conversion coefficients. This grayscale image is subsequently analyzed by plots of its intensity histogram (the number of pixels plotted against their respective brightness levels) and probability distribution of the brightness, and by calculation of various parameters, including average brightness, standard deviation, variance, minimal and maximal brightness, mode, skewness and kurtosis of the histogram and the median of the probability distribution. The program allows the selection of a region of interest (ROI) in the image and/or lower and upper threshold levels for analysis (e.g., to permit the removal of a constant background signal). The combination of these analysis features in a single program allows fast analysis of relatively large sets of image data. The results of the analysis of multiple images can be saved for data transfer and plotting in other programs. The program was written especially for comparisons of the fluorescence levels in microscopic images and has been applied for such applications.. , Situação: Em andamento; Natureza: Desenvolvimento. , Alunos envolvidos: Graduação: (0) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Oliver Holub - Coordenador / Sérgio T. Ferreira - Integrante., Financiador(es): Conselho Nacional de Desenvolvimento Científico e Tecnológico - Bolsa.

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