Jasmin Lindner

Idiomas

Áreas de atuação

Participação em eventos

Produções bibliográficas

• KRONENBERGER, THALES ; LINDNER, JASMIN ; MEISSNER, KAMILA A. ; ZIMBRES, FLÁVIA M. ; CORONADO, MONIKA A. ; SAUER, FRANK M. ; SCHETTERT, ISOLMAR ; WRENGER, CARSTEN . Vitamin B6-Dependent Enzymes in the Human Malaria Parasite Plasmodium falciparum: A Druggable Target?. BIOMED RES INT , v. 2014, p. 1-11, 2014.

• LINDNER, JASMIN ; MEISSNER, KAMILA ANNA ; SCHETTERT, ISOLMAR ; WRENGER, CARSTEN . Trafficked Proteins-Druggable in Plasmodium falciparum?. International Journal of Cell Biology , v. 2013, p. 1-13, 2013.

Projetos de pesquisa

• 2012 - 2012

  Nutrient Acquisition and endosome formation in the human malaria parasite Plasmodium falciparum, Descrição: The malaria parasite Plasmodium falciparum is proliferation within the human red blood cells. Thereby the parasite depends of the host metabolism in terms of carbohydrate, cofactor and amino acids (etc) supply. Further many nutrients, which are imported from the host, are chemically modified e.g. by phosphorylation. These phosphorylated molecules hinder the uptake procedure since the parasite has to re-modify these molecules intracellularly to suit its own needs. Recently, a secreted phosphatase (PfSAP) has been identified which is proposed to be responsible for extracellular de-phosphorylation. However, the protein is trafficked by endosomal/lysosomal vesicle transport to the digestive vacuole of the parasite. The mechanism of this endosomal/lysosomal initiation and subsequent vesicle transport is poorly understood in P. falciparum. Within this project C-terminal part of PfSAP will be analysed for substrate acceptance of serine/threonine protein kinases which is mandatory for the endosomal/lysosomal initiation. Further the respective proteins shall be visualised by fluorescence microscopy of GFP chimeras. The discovery of proteins involved in endosomal/lysosomal vesicle transport might be promising novel drug targets, since the parasite relies on metabolic environment its host.. , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Jasmin Lindner - Integrante / Carsten Wrenger - Coordenador / Jörg Ganzhorn - Integrante., Financiador(es): Deutscher Akademischer Austauschdienst - Bolsa.

• 2012 - Atual

  Anaylsis of the haemoglobin catabolism of Plasmodium falciparum using transgenic parasites, Descrição: Highly proliferating cells such as the malaria parasite Plasmodium falciparum necessitate an accelerated level of carbohydrates for energy supply for maintenance of intracellular processes. Besides sugars one of these sources are amino acids which are imported from the host cell plasma and by digestion of haemoglobin within the food vacuole. During haemoglobin digestion heme is going to be released which needs to be detoxified into hemozoin. Humans who harbours erythrocytic diseases related to the haemoglobin architecture such as sickle cell disease or alpha- or beta thalassaemia gain a protective advantage while infected with the malaria pathogen. Within this project it is intended to get insights into the mode of action of parasite growth within genetically different erythrocytes will be analysed by focussing on the plasmodial haemoglobin catabolic pathway using transgenic parasites. Furthermore, the respective localization of catabolic enzymes will be investigated by fluorescence microscopy using green-fluorescent-protein- chimeras and selective plasmodial haemoglobin catabolic enzymes will be recombinantly expressed in order to obtain structural details. The parasite´s nutrient metabolism shelters a high potential for the development of novel drug targets. Therefore a clear understanding of the occurring haemoglobin digestion and the protective nature of haemoglobin variants is essential.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Jasmin Lindner - Integrante / WRENGER, CARSTEN - Coordenador., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Bolsa.

• 2010 - 2010

  Tolerância de hipoxia no cerébro de focas de capuz, Descrição: Deep diving mammals are regularly exposed to limited oxygen availability (hypoxia). The hooded seal (Cystophora cristata) is able to dive up to 1000 meters and can stay submerged for more than 50 minutes. Apart from higher expression levels of hemoglobin in blood cells and myoglobin in skeletal muscles, many physiological adaptations to hypoxia are known in these animals. However, less is known about the biochemical mechanisms of hypoxemic tolerance in the brain. Recently it has been shown that hooded seals possess a longer neuronal activity than brain neurons of mice. In addition, a shift of neuroglobin (Ngb) and the oxidative metabolism from neurons into glial cells (astrocytes) was discovered. Thus, a higher protection of neurons against hypoxia and presumably reactive oxygen species (ROS) was assumed. By using the methods of real-time PCR and western blotting we investigate transcription and expression levels of Ngb and glycolytic enzymes in adult hooded seal brain. Furthermore, we analyze the expression of superoxide dismutase 2 (SOD2), which detoxifies ROS. Preliminary results indicate a higher amount of SOD2 in the brain of the hooded seal than in those of the ferret (Mustela putorius furo), suggesting an intrinsically higher antioxidative capacity of the seals brain.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Jasmin Lindner - Coordenador / Marco Schneuer - Integrante / Thorsten Burmester - Integrante., Financiador(es): Universidade de Hamburgo - Auxílio financeiro.