Maria Gabriela Kramer Xavier

Graduada em Licenciatura em Ciencias Biologicas pela Universidad Complutense de Madrid (1993) e Doutorado em Bioquimica e Biologia Molecular pelo Centro de Investigaciones Biologicas, Madrid, Espanha (1997). Realizou seu Pós-Doutorado na área de Microbiologia na Faculdade de Medicina da Universidade de Pittsburgh, Pennsylvania, USA (1997-1999). Posteriormente, integrou-se ao Departamento de Medicina Interna da Universidade de Navara em Pamplona, Espanha, para trabalhar com desenvolvimento de vetores na terapia gênica (1999-2000). Em seguida, integrou-se ao staff de professores e pesquisadores da Unidad de Hepatología y Terapia génica del Centro de Investigacion Médica Aplicada en Pamplona, onde obteve vários projetos para pesquisar o desenvolvimento de promotores específicos de tecido, sistemas de regulação da expressão de genes terapêuticos, modelos animais de câncer de fígado e produção de vetores lentivirais (2001-2007). Após, realizou estudos sobre o efeito de inibidores de deacetilasas de hisonas no tratamento do câncer de mama e de fígado em Peter Mac Callum Cancer Center de Melbourne, Australia (2007-2009). Foi professor vsitante no Instituto de Biologia Molecular do Paraná (Fio Cruz), Curitiba, Brasil, durante 2 meses (2006 e 2008) e fez parte do projeto de pesquisa "Isolamento, diferenciação e transplante de células tronco adultas: Estudos experimentais em ratos", financiado por CNPq e Ministério de Saúde. Em 2009, foi colaboradora do Instituto Pasteur-Montevideo, Uruguai, até 2010. Atualmente é Professor Adjunto Titular do Departamento de Desarrollo Biotecnológico da Faculdade de Medicina da Universidad de la Republica e trabalha com pesquisas na avaliação de estratégias na terapia gênica imunoestimuladoras para o tratamento do câncer de mama.

Informações coletadas do Lattes em 10/11/2022

Acadêmico

Formação acadêmica

Doutorado em Bioquimica y Biologia Molecular

1993 - 1997

Centro de Investigaciones Biológicas
Título: Mechanism of lagging-strand replication of plasmid pMV158 of Streptococcus pneumoniae
Orientador: Manuel Espinosa Padron
Bolsista do(a): Celestino Mutis (Agencia Espanhola de Cooperacion Internacional). Palavras-chave: DNA replication; RNA polymerase; rolling circle plasmids.Grande área: Ciências Biológicas / Área: Microbiologia. Grande Área: Ciências Biológicas / Área: Bioquímica. Grande Área: Ciências Biológicas / Área: Genética.

Graduação em Licenciatura en Ciencias Biologicas

1989 - 1993

Universidad Complutense de Madrid
Bolsista do(a): Instituto de Cooperacion Iberoamericano.

Pós-doutorado

2000 - 2001

Pós-Doutorado. , Universidad de Navarra, UNA, Espanha. , Bolsista do(a): Gobierno Vasco. , Grande área: Ciências Biológicas / Área: Genética / Subárea: Gene Therapy. , Grande Área: Ciências Biológicas / Área: Genética / Subárea: Gene Therapy / Especialidade: Liver specific promoters. , Grande Área: Ciências Biológicas / Área: Genética / Subárea: Gene Therapy / Especialidade: Non-viral vectors.

1997 - 1999

Pós-Doutorado. , University of Pittsburgh, PITT, Estados Unidos. , Bolsista do(a): National Institute of Health. , Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: Bioquímica dos Microorganismos.

Formação complementar

2010 - 2010

Extensão universitária em Diplomatura de Formación Docente en Medicina. (Carga horária: 100h). , Facultad de Medicina, CLAEH.

2010 - 2010

Segunda Jornada de Capacitación Portal Timbo. (Carga horária: 8h). , Agencia Nacional de Investigación e Innovación.

Idiomas

Inglês

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Espanhol

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Português

Compreende Bem, Fala Razoavelmente, Lê Bem, Escreve Razoavelmente.

Alemão

Compreende Razoavelmente, Fala Pouco, Lê Razoavelmente, Escreve Pouco.

Participação em eventos

SOCIEDAD URUGUAYA DE BIOCIENCIAS. Mesa redonda sobre Vectores Virales. 2012. (Congresso).

SOCIEDAD URUGUAYA DE BIOCIENCIAS. Terapia génica del cáncer de mama. 2011. (Congresso).

JORNADAS DE LA SOCIEDAD URUGUAYA DE BIOCIENCIAS. Terapia Génica del cáncer empleando vectores plasmídicos y bacterianos. 2010. (Congresso).

INTERNATIONAL PLASMID BIOLOGY CONFERENCE 2010. Development of plasmid- and bacterial-based vectors for the therapy of cancer. 2010. (Congresso).

JORNADAS DE LA SOCIEDAD DE BIOQUÍMICA Y BIOLOGÍA MOLECULAR. Terapia Génica del Carcinoma Hepatocelular y Diagnóstico de Tumores hepáticos con micro-Pet. 2009. (Congresso).

XXXIII CONGRESO DE LA ASOCIACIÓN ESPAÑOLA PARA EL ESTUDIO DEL HÍGADO. Efecto antitumoral de vectores basados en el virus del Bosque de Semliki que expresan IL-12 en tumores desarrollados en el hígado. 2008. (Congresso).

20. ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH. Evaluation of interleukin-12-based gene therapy in a transgenic mice model of hepatocellular carcinoma. 2007. (Congresso).

II SIMPÓSIO INTERNACIONAL TERAPIAS AVANÇADAS E CÉLULAS-TRONCO. Transduction Efficiency of Human Mesenchymal Stem Cells From Different Sources: Tool to Follow Differentiation Up. 2007. (Congresso).

XXXI CONGRESO DE LA ASOCIACIÓN ESPAÑOLA PARA EL ESTUDIO DEL HÍGADO. Efecto antitumoral de la expresión controlada de interleuquina-12 en un modelo murino de metástasis hepáticas. 2006. (Congresso).

14th ANNUAL CONGRESS OF EUROPEAN SOCIETY OF GENE THERAPY. Sustained expression of interleukin-12 causes regression of liver metastases in a murine model. 2006. (Congresso).

INTERNATIONAL SYMPOSIUM ON PLASMID BIOLOGY. Gene therapy of cancer using plasmid-based vectors. 2006. (Congresso).

56th ANNUAL MEETING AMERICAN ASSOCIATION FOR THE STUDY OF LIVER DISEASES. Gene therapy of hepatocellular carcinoma using a plasmid vector to control intrahepatic expression of interleukin-12 and diagnosis of liver tumors with microPET. 2005. (Congresso).

INTERNATIONAL SOCIETY FOR INTERFERON AND CYTOKINE RESEARCH ANNUAL MEETING. Kinetics of gene regulation in the liver using drug-inducible systems can be affected by cytokine transgene expression. 2005. (Congresso).

INTERNATIONAL SOCIETY OF GASTROINTESTINAL ONCOLOGY ANNUAL MEETING. Antitumor effect of intrahepatic interleukin-12 expression in murine models of primary and matastatic liver cancer. 2005. (Congresso).

III REUNIÓN DE LA SOCIEDAD ESPAÑOLA DE TERAPIA GÉNICA. An oncolytic adenovirus controlled by a modified telomerase promoter Is attenuated In telomerase-negative cells, but shows reduced activity In cancer cells. 2005. (Congresso).

XXIX CONGRESO DE LA ASOCIACIÓN ESPAÑOLA PARA EL ESTUDIO DEL HÍGADO. Development of plasmid vectors to transfer IL-12 genes to the liver and its application for the treatment of hepatic tumors. 2004. (Congresso).

11th ANNUAL CONGRESS OF THE EUROPEAN SOCIETY OF GENE THERAPY. The inducible expression of Interleukin-12 using a gutless adenoviral vector eradicates liver metastases in mice. 2003. (Congresso).

38th ANNUAL MEETING OF THE EUROPEAN ASSOCIATION FOR THE STUDY OF THE LIVER. Engineering tumor specific replicating adenoviruses as therapeutic agents for treatment of hepatocellular carcinoma. 2003. (Congresso).

XXVIII MEETING OF THE SPANISH ASSOCIATION FOR THE STUDY OF THE LIVER. Optimization of a system to regulate interleukin-12 expression in the liver. 2003. (Congresso).

5TH ANNUAL MEETING OF THE AMERICAN SOCIETY OF GENE THERAPY. Transcriptional targeting of heterologous genes to the liver using chimeric promoters: in vitro and in vivo comparative study. 2002. (Congresso).

INTERNATIONAL SYMPOSIUM ON PLASMID BIOLOGY. Role of RNA Polymerase-Single Strand origin Interactions in determining the host range of rolling-circle plasmids from Grampositive bacteria. 1999. (Congresso).

INITIATION OF REPLICATION IN PROKARYOTIC EXTRACHROMOSOMAL ELEMENTS. Rolling circle replication of bacterial plasmids: Mechanism of lagging-strand synthesis. 1998. (Congresso).

Orientou

Martín Masner

Tratamientos del cáncer de mama empleando bacterias atenuadas; Início: 2011; Dissertação (Mestrado profissional em Medicina) - Facultad de Medicina, Universidad de la República; (Orientador);

Natalia Mazza

Caracterización de tumores mamarios inducidos químicamente en ratas; Início: 2011; Trabalho de Conclusão de Curso (Graduação em Biologia Molecular) - Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Udelar, FMV-ANII2011; (Orientador);

Maider Zabala Ugalde

Optimization of the Tet-on system to regulate interleukin 12 expression in the liver for the treatment of hepatic tumors; 2005; Tese (Doutorado em Hepatology and Gene Therapy) - Centro de Investigacion Medica Aplicada,; Orientador: Maria Gabriela Kramer Xavier;

Guanghua Yang

Modificación de células progenitoras adultas con vectores lentivirales para emplearlas en el tratamiento de tumores hepáticos; 2005; Tese (Doutorado em Biologia Molecular) - Universidad de Navarra,; Coorientador: Maria Gabriela Kramer Xavier;

Mercedes Reboredo Proll

2004; Centro de Investigacion Medica Aplicada,; Maria Gabriela Kramer Xavier;

Rodrigo Gonzalez

Impacto de la estabilidad plasmídica en el diseño de vectores bacterianos; 2010; Trabalho de Conclusão de Curso; (Graduação em Biologia Molecular) - epartamento de Desarrollo Biotecnológico, Instituto de Higiene, Udela; Orientador: Maria Gabriela Kramer Xavier;

Leire Garate

Producción de vectores lentivirales de tercera generaciíon que expresan GFP; 2005; Orientação de outra natureza; (Bioquimica) - Universidad de Navarra; Orientador: Maria Gabriela Kramer Xavier;

Nerea Razquin

Preparación y clonaje de DNA, Western y Southern Blots; 2003; Orientação de outra natureza; (Bioquimica) - Universidad de Navarra; Orientador: Maria Gabriela Kramer Xavier;

Produções bibliográficas

MORENO, M. ; KRAMER, M. G ; YIM, L. ; CHABALGOITY, J. A. . Salmonella as Live Trojan Horse for Vaccine Development and Cancer Gene Therapy. Current Gene Therapy , v. 10, p. 56-76-76, 2010.
Zabala, Maider ; Alzuguren, Pilar ; Benavides, Carolina ; Crettaz, Julien ; Gonzalez-Aseguinolaza, Gloria ; Ortiz de Solorzano, Carlos ; Gonzalez-Aparicio, Manuela ; Kramer, Maria ; Prieto, Jesus ; Hernandez-Alcoceba, Ruben . Evaluation of bioluminescent imaging for noninvasive monitoring of colorectal cancer progression in the liver and its response to immunogene therapy. Molecular Cancer , v. 8, p. 2, 2009.
Reboredo, Mercedes ; Kramer, Maria Gabriela ; Smerdou, Cristian ; Prieto, Jesús ; Rivas, Javier De Las . Transcriptomic Effects of Tet-On and Mifepristone-Inducible Systems in Mouse Liver. Human Gene Therapy , v. 19, p. 1233-1248, 2008.
Reboredo, M ; Zabala, M ; Mauleon, I ; De Las Rivas, J ; Kreppel, F ; Kochanek, S ; Prieto, J ; Hernandez-Alcoceba, R ; Kramer, M G . Interleukin-12 inhibits liver-specific drug-inducible systems in vivo. Gene Therapy (Basingstoke) , v. 15, p. 277-288, 2007.
BARAJAS, M ; FRANCHI, F ; CLAVEL, C ; ARANGUREN, X ; KRAMER, M ; ABIZANDA, G ; MERINO, J ; MORENO, C ; GARATE, L ; GUITART, A . Multipotent Adult Progenitor Cells (MAPC) contribute to hepatocarcinoma neovasculature?. Biochemical and Biophysical Research Communications (Print) , v. 364, p. 92-99, 2007.
Zabala, M ; LASARTE, J ; PERRET, C ; SOLA, J ; BERRAONDO, P ; ALFARO, M ; LARREA, E ; Prieto, J ; KRAMER, M . Induction of immunosuppressive molecules and regulatory T cells counteracts the antitumor effect of interleukin-12-based gene therapy in a transgenic mouse model of liver cancer?. Journal of Hepatology , v. 47, p. 807-815, 2007.
Guan M. ; Rodriguez-Madoz J.R. ; Alzuguren P. ; Gomar C. ; Kramer, M G ; Kochanek S. ; Prieto J. ; Smerdou C. ; Qian C. . Increased Efficacy and Safety in the Treatment of Experimental Liver Cancer with a Novel Adenovirus-Alphavirus Hybrid Vector. Cancer Research (Chicago, Ill.) , v. 66, p. 1620-1629, 2006.
Bortolanza, S. ; Qian, C. ; Kramer, M. G. ; Gomar, C. ; Prieto, J. ; Farinati, F. ; Hernandez-Alcoceba, R. . An oncolytic adenovirus controlled by a modified telomerase promoter is attenuated in telomerase-negative cells, but shows reduced activity in cancer cells. Journal of Molecular Medicine , v. 83, p. 736-747, 2005.
Kramer, M. G. ; Hernandez-Alcoceba, Ruben ; Qian C. ; Prieto J. . Evaluation of hepatocellular carcinoma models for preclinical studies. Drug Discovery Today. Disease Models , v. 2, p. 41-49, 2005.
WADDINGTON, S ; KRAMER, M ; HERNANDEZALCOCEBA, R ; BUCKLEY, S ; THEMIS, M ; COUTELLE, C ; Prieto, J . gene therapy: current challenges and perspectives. Molecular Therapy , v. 11, p. 661-676, 2005.
Prieto, Jesus ; Qian, Cheng ; Hernandez-Alcoceba, Ruben ; Gonzalez-Aseguinolaza, Gloria ; Mazzolini, Guillermo ; Sangro, Bruno ; KRAMER, M. G . Gene therapy of liver diseases. Expert Opinion on Biological Therapy , v. 4, p. 1073-1091, 2004.
Zabala, M ; Wang L. ; Hernandez-Alcoceba, R. ; Hillen W. ; Qian, C. ; Prieto J. ; Benavides, Carolina ; Kramer, M. G. . Optimization of the Tet-on system to regulate interleukin 12 expression in the liver for the treatment of hepatic tumors. Cancer Research (Chicago, Ill.) , v. 64, p. 2799-2804, 2004.
Ruz N. ; Zabala, M ; Kramer, M G ; Campanero M. A ; Dios M. C ; Blanco-Prieto M. J . Rapid and simple determination of doxycycline in serum by high performance liquid chromatography. Application to nanoparticulate drug delivery systems. Journal of Chromatography (Print) , v. 1031, p. 295-301, 2004.
WANG, L ; Hernandez-Alcoceba, R. ; Shankar V. ; Zabala, M ; Kochanek, S ; Sangro B ; Kramer, M. G. ; Prieto J. ; Qian C. . Prolonged and inducible transgene expression in the liver using gutless adenovirus: A potential therapy for liver cancer. Gastroenterology (New York, N.Y. 1943) , v. 126, p. 278-289, 2004.
KRAMER, M. G ; BARAJAS, M ; Razquin N. ; BERRAONDO, P ; Rodrigo M. ; Wu C. ; Qian, C. ; Fortes P. ; Prieto J. . In vitro and in vivo comparative study of chimeric liver-specific promoters. Molecular Therapy , v. 7, p. 375-385, 2003.
Chang, T.-L. ; Naqvi A. ; Anand S. P. ; KRAMER, M. G ; Munshi R. ; Khan S.A. . Biochemical Characterization of the Staphylococcus aureus PcrA Helicase and Its Role in Plasmid Rolling Circle Replication. The Journal of Biological Chemistry (Print) , v. 277, p. 45880-45886, 2002.
Chang, T.-L. ; Kramer, M. G. ; Ansari R. A. ; Khan S.A. . Role of individual monomers of a dimeric initiator protein in the initiation and termination of plasmid rolling circle replication. The Journal of Biological Chemistry (Print) , v. 275, p. 13529-13534, 2000.
KRAMER, M. G ; Espinosa M. ; Misra T. ; Khan S.A. . Characterization of a single-strand origin, ssoU, required for broad host range replication of rolling-circle plasmids. Molecular Microbiology , v. 33, p. 466-475, 1999.
Acebo P. ; Hernandez A. ; Kramer, M. G. ; Espinosa M. ; del Solar G. . Identification of a new gene in the streptococcal plasmid pLS1: the rnaI gene. Plasmid (San Diego. Print) , v. 40, p. 214-224, 1998.
Kramer, M. G. ; Espinosa M. ; Misra T. ; Khan S.A. . Lagging-strand replication of rolling-circle plasmids: Specific recognition of the ssoA-type origins in different gram-positive bacteria. Proceedings of the National Academy of Sciences of the United States of America , v. 95, p. 10505-10510, 1998.
Kramer, M. G. ; Khan S.A. ; Espinosa M. . Lagging strand replication from the ssoA origin of plasmid pMV158 in Streptococcus pneumoniae: in vivo and in vitro influence of mutations in two conserved ssoA regions. Journal of Bacteriology (Print) , v. 180, p. 83-89, 1998.
Kramer, M G ; Khan S.A. ; Espinosa M. . Plasmid rolling circle replication: identification of the RNA polymerase-directed primer RNA and requirement for DNA polymerase I for lagging strand synthesis, v. 16, p. 5784-5795, 1997.
Kramer, M. G. ; del Solar G. ; Espinosa M. . Lagging-strand origins of the promiscuous plasmid pMV158 physical and functional characterization. Microbiology (Reading) , v. 141, p. 655-662, 1995.
del Solar G. ; KRAMER, G. ; Ballester S. ; Espinosa M. . Replication of the promiscuous plasmid pLS1: a region encompassing the minus origin of replication is associated with stable plasmid inheritance. MGG. Molecular & General Genetics (Print) , v. 241, p. 97-105, 1993.
Kramer, M. G. . Orígenes de replicación de la cadena retrasada del plásmido pMV158. Madrid: Servicio de Publicaciones de la Universidad Complutense de Madrid, 2004.
Prieto J. ; Qian C. ; Herraiz M. ; Hernandez-Alcoceba, R. ; Kramer, M. G. ; Smerdou C. ; Mazzolini, Guillermo ; Melero I. ; Sangro B. . Gene therapy of Liver Cancer: the Present and the Future. State of the art in Hepatology: Molecular and Cell Biology. Germany: H. Blum and M. Manns. Kluwer Academic Publishers, 2004, v. , p. 148-.

Projetos de pesquisa

2006 - 2007

Combination of gene therapy with interleukin-12 and an histone deacetylase inhibitor for the treatment of liver cancer in a transgenic mice model of hepatocellular carcinoma, Descrição: The aim of this project was to evaluate the antitumoral efficacy of a gene therapy- based protocol that consists of the expression of interleukin-12 (IL-12) in the liver for a long-time period, alone, or in combination with an analog of the histone deasetylase inhibitor sodium butyrate (HA-NaB). We used an animal model that develops liver tumors with an histo-pathology comparable to human hepatocellular carcinoma. Our results showed that IL-12 alone can inhibit tumor growth in 40% of the cases, while HA-NaB has no therapeutic effect by itself. Interestingly, the therepautic potential of IL-12 could be inhibited by HA-NaB, when given both agents in combination.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Maria Gabriela Kramer Xavier - Coordenador / Maider Zabala Ugalde - Integrante / Mercedes Reboredo Proll - Integrante.
2005 - 2007

Isolation, differentiation and transplant of adult progenitor cells: Experimental study in rats., Situação: Concluído; Natureza: Pesquisa. , Integrantes: Maria Gabriela Kramer Xavier - Integrante / Marco Aurelio Krieger - Coordenador / Bruno Dallagiovanna - Integrante / Samuel Goldenderg - Integrante / Alejandro Correa - Integrante / Paulo Brofman - Integrante / Alessandra Senegaglia - Integrante / Carmen Rebelatto - Integrante / Patricia Shigunov - Integrante.
2005 - 2007

Aislamiento, diferenciación y transplante de células progenitoras adultas: Estudio experimental en ratas, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Maria Gabriela Kramer Xavier - Integrante / Marco Aurelio Krieger - Coordenador.
2004 - 2007

Gene therapy of cancer: Evaluation of the effect on endogenous gene expression of chimeric trans-activators used to induce therapeutic gene expression in the liver, Descrição: Control of transgene expression from long-term expression vectors can be achieved with transcription inducible systems. The two most commonly used systems employ doxycycline or mifepristone as the drug activating a silent trans-activator, which is expressed from a constitutive promoter. We evaluated the alterations provoked by constitutive expression in the liver of rtTA2(S)-M2 (rtTA2; second-generation reverse tetracycline-controlled trans-activator) and GLp65, which are the trans-activators of the doxycyline- and mifepristone-inducible systems, respectively. To this end we performed transcriptomic analysis of mice expressing these trans-activators in the liver over 1 month. Our results showed absence of toxicity in treated animals and the liver transcriptome profile indicate that the two inducible systems have little impact on the endogenous gene expression.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Maria Gabriela Kramer Xavier - Coordenador / Maider Zabala Ugalde - Integrante / Mercedes Reboredo Proll - Integrante / Javier de las Rivas - Integrante.
2004 - 2007

Endothelial progenitor cells and cells derived from multipotent adult progenitor cells as vehicles for genes with antitumoral potential, Descrição: The use of endothelial progenitor cells (EPC) as a vehicle of therapeutic genes is an attractive approach for the development of new antitumoral strategies based on gene therapy. The aim of our study was to assess the potential of EPC to be modified by lentiviral vector transduction and to be recruited to areas of tumor vasculogenesis. In this project, we have foccused on the development of lentiviral vectors carrying the IL-12 gene under the control of a doxycycline-based inducible system. In addition, we have performed a comparative study of endothelial-specific promoters for the constitutive expression of trasgenes in endothelial-derived cells.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Maria Gabriela Kramer Xavier - Integrante / Cheng Qian - Coordenador / Miguel Barajas - Integrante / Iñigo Narvaiza - Integrante / Jesus Prieto - Integrante / Guanhua Yang - Integrante.
2001 - 2004

Development of a system to direct and control gene expression in the liver and its application for the treatment of liver cancer with interleukin 12, Descrição: Interleukin 12 (IL-12) is a potent antitumoral cytokine, but it can be toxic at high doses. Therapy of liver tumors might benefit from the use of vectors enabling tight control of IL-12 expression in hepatic tissue for long periods of time. To this aim, we have improved the Tet-on system by modifying the minimal region of the inducible promoter and adjusting the level of the trans-activator using liver-specific promoters with graded activities. The resulting vectors allowed hepato-specific gene regulation with lower basal activity and higher inducibility compared with the original system in the absence of repressor molecules. The antitumor effect of IL-12 was evaluated in a mouse model of metastatic colon cancer to the liver. Complete eradication of liver metastasis and prolonged survival was observed in all mice receiving Dox for 10 days.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Maria Gabriela Kramer Xavier - Coordenador / Maider Zabala Ugalde - Integrante / Cheng Qian - Integrante / Miguel Barajas - Integrante / Jesus Prieto - Integrante.
2001 - 2003

Regulation of therapeutic gene expression in the liver, Descrição: Targeting therapeutic genes to the liver is essential to improve gene therapy protocols of hepatic diseases and of some hereditary disorders. Transcriptional targeting can be achieved using liver-specific promoters. In this study we have made chimeric constructs combining promoter and enhancer regions of the albumin, alpha 1-antitrypsin, hepatitis B virus core protein, and hemopexin genes. Tissue specificity, activity, and length of gene expression driven from these chimeric regulatory sequences have been analyzed in cultured cells from hepatic and nonhepatic origin as well as in mice livers and other organs. We found that among the various chimeric constructs tested in this work, the alpha1-antitrypsin promoter alone or linked to the albumin or hepatitis B enhancers is the most potent in directing stable gene expression in liver cells.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Maria Gabriela Kramer Xavier - Coordenador / Miguel Barajas - Integrante / Jesus Prieto - Integrante / Puri Fortes - Integrante / Nerea Razquin - Integrante.
1996 - 2000

Plasmid pT181DNA replication in Staphylococcus aureus, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Maria Gabriela Kramer Xavier - Integrante / Saleem A. Khan - Integrante.
1994 - 1997

Expresión Génica y su control: Sistemas modelo de plásmidos promiscuos y bacterias Gram-positivas, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Maria Gabriela Kramer Xavier - Integrante / Manuel Espinosa Padrón - Integrante.

Prêmios

2007

Award for the best poster presentation, 42th Annual Meeting of the European Association of the Study for the Liver.

2004

Award for best poster presentation (basic biology category), XXIX Annual Meeting of the Spanish Association for the Study of the Liver Diseases.

2003

Award for the second best poster presentation, 11th Annual Congress of the European Society of Gene Therapy.

2003

Award for best poster presentation (basic biology category)., XXVIII Annual Meeting of the Spanish Association for the Study of the Liver Diseases.

1997

Extraordinary award for best Doctoral Thesis 1996-1997, Facultad de Ciencias-Universidad Complutende de Madrid.

Histórico profissional

Endereço profissional

Facultad de Medicina, Universidad de la República. , Avda. A. Navarro 3051, Parque Batlle, Montevideo, - Uruguai - Caixa-postal: 11600, Telefone: (598) 24871288, Ramal: 1123, Fax: (598) 4873073, URL da Homepage:

Experiência profissional

2010 - Atual

Facultad de Medicina, Universidad de la República

Vínculo: , Enquadramento Funcional: Profesor Adjunto, Carga horária: 40, Regime: Dedicação exclusiva.

2009 - 2010

Instituto Pasteur-Montevideo

Vínculo: Pasante honorario, Enquadramento Funcional: Colaborador, Carga horária: 20

Outras informações:
Research Line: Glycosyltransferases as molecular markers of cancer progression

2009 - 2009

La Trobe University

Vínculo: Colaborador, Enquadramento Funcional: Research Assistant, Carga horária: 30

Outras informações:
Research line: Strategies for cDNAs cloning into lentiviral and plasmid vectors

2008 - 2008

Fundação Oswaldo Cruz, Instituto Carlos Chagas

Vínculo: Professor vistante, Enquadramento Funcional: Pesquisador visitante, Carga horária: 40

Outras informações:
Research line: Project discussion, data evaluation and lentiviral vectors production

2006 - 2008

Peter Mac Callum Cancer Centre

Vínculo: Professor vistante, Enquadramento Funcional: Visitor Research Fellow, Carga horária: 40

Outras informações:
Reserach line: Evaluation of the antitumoral effect of histone deacetylases inhibitors in human hepatocellular carcinoma cell lines

2006 - 2006

Instituto de Biologia Molecular do Paraná

Vínculo: Professor vistante, Enquadramento Funcional: Pesquisador visitante, Carga horária: 40

Outras informações:
Research line: lentiviral vectors production

2001 - 2006

Centro de Investigacion Medica Aplicada

Vínculo: Celetista formal, Enquadramento Funcional: Staff Investigator, Carga horária: 40, Regime: Dedicação exclusiva.

Outras informações:
Research lines: Optimization of inducible systems to control the expression of therapeutic genes in the liver. Evaluation of the antitumoral activity of interleukine 12 in different murine models of liver cancer. Analysis of the transcriptomic effect of chimeric proteins with trans-activator function in mouse liver. Design and production of lentiviral vectors carrying gene regulatory systems.

1997 - 2000

University of Pittsburgh

Vínculo: Research Associate, Enquadramento Funcional: Research Associate, Carga horária: 40, Regime: Dedicação exclusiva.

Outras informações:
Research lines: Mechanism of replication of rolling-circle plasmids

1993 - 1997

Centro de Investigaciones Biológicas

Vínculo: Bolsista, Enquadramento Funcional: Pre-doctoral, Carga horária: 40, Regime: Dedicação exclusiva.

Outras informações:
Research lines: Lagging-strand replication of rolling circle plasmids. Plasmid stability in Gram (+) and Gram (-) bacterial hosts.