Ernane Cláudio de Souza Júnior

Pós-doutorado

Formação complementar

Idiomas

Áreas de atuação

Organização de eventos

Participação em eventos

Produções bibliográficas

• FERNÁNDEZ-GALLEGUILLOS, CARLOS ; QUESADA-ROMERO, LUISA ; PUERTA, ADRIÁN ; PADRÓN, JOSÉ M. ; SOUZA, ERNANE ; ROMERO-PARRA, JAVIER ; SIMIRGIOTIS, MARIO J. . UHPLC-MS Chemical Fingerprinting and Antioxidant, Antiproliferative, and Enzyme Inhibition Potential of Gaultheria pumila Berries. METABOLITES , v. 11, p. 523, 2021.

• CRASS, RYAN L. ; AL NAIMI, TAMARA ; WEN, BO ; SOUZA, ERNANE ; MURRAY, SUSAN ; PAI, MANJUNATH P. ; JIA, SHIJING . Pharmacokinetics of Polymyxin B in Hospitalized Adults with Cystic Fibrosis. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY , v. 65, p. e0079221, 2021.

• HE, MIAO ; SOUZA, ERNANE ; MATVEKAS, ALEKSAS ; CRASS, RYAN L. ; PAI, MANJUNATH P. . Alteration in Acute Kidney Injury Potential with the Combination of Vancomycin and Imipenem-Cilastatin/Relebactam or Piperacillin/Tazobactam in a Preclinical Model. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY , v. 65, p. e02141-20, 2021.

• GUIMARÃES, FLÁVIA DE PAULA GONÇALVES ; JÚNIOR, ERNANE CLÁUDIO DE SOUZA ; JÚNIOR, CARLOS ALBERTO MOURÃO ; NASCIMENTO, JORGE WILLIAN LEANDRO . Política de proibição da dipirona: uma reflexão / Dipyrone ban policy: a reflection. Brazilian Journal of Health Review , v. 4, p. 11007-11019, 2021.

• SOUZA, ERNANE ; FELTON, JEREMY ; CRASS, RYAN L. ; HANAYA, KENGO ; PAI, MANJUNATH P. . Development of a sensitive LC-MS/MS method for quantification of linezolid and its primary metabolites in human serum. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS , v. 178, p. 112968, 2020.

• SOUZA, ERNANE ; CRASS, RYAN L. ; FELTON, JEREMY ; HANAYA, KENGO ; PAI, MANJUNATH P. . Accumulation of Major Linezolid Metabolites in Patients with Renal Impairment. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY , v. 64, p. 1, 2020.

• SOUZA, ERNANE ; CHO, KATHERINE H. ; HARRIS, SHELBY T. ; FLINDT, NAOMI R. ; WATT, RICHARD K. ; PAI, AMY BARTON . Hypoxia-inducible factor prolyl hydroxylase inhibitors: a paradigm shift for treatment of anemia in chronic kidney disease?. EXPERT OPINION ON INVESTIGATIONAL DRUGS , v. 1, p. 1-14, 2020.

• Silvana Cartaxo da Costa Urtiga ; ALVES, V. ; MELO, C. O. ; LIMA, M. N. ; SOUZA, ERNANE ; CUNHA, A. P. ; RICARDO, N. ; OLIVEIRA, E. E. ; EGITO, E. S. . Xylan microparticles for controlled release of mesalamine: Production and physicochemical characterization. CARBOHYDRATE POLYMERS , v. 250, p. 116929, 2020.

• PAI, A. B. ; MCGUIRE, M. D. ; DAVIDGE, K. N. ; DEAN, M. C. ; COSTELLO, G. N. ; SOUZA, ERNANE ; MUKHERJEE, S. ; HEUNG, M. ; YEVZLIN, A. S. ; YESSAYAN, L. T. . Lipoteichoic Acid as a Potential Noninvasive Marker of Biofilm in Dialysis Access. ASAIO JOURNAL , v. 1, p. 1, 2019.

• de Souza, Ernane C. ; MATOS, DALYARA M. ; VIANA, MILAINY R. ; ALVIM, MARCELA C.O. ; BONFANTE, HERVAL L. ; PINTO, ALEXANDRE F. ; NASCIMENTO, JORGE W.L. . Evaluation of hematological alterations after therapeutic use of dipyrone in healthy adults: a prospective study. JOURNAL OF BASIC AND CLINICAL PHYSIOLOGY AND PHARMACOLOGY , v. 29, p. 385-390, 2018.

• DESOUZA, ERNANE C. ; ROMERO-ORTEGA, M. ; OLIVO, H. F. . Lipase-mediated selective acetylation of primary alcohols in ethyl acetate. TETRAHEDRON LETTERS , v. 59, p. 287, 2018.

• MALIK, MUHAMMAD ; MUSTAEV, ARKADY ; SCHWANZ, HEIDI A. ; LUAN, GAN ; SHAH, NIRALI ; OPPEGARD, LISA M. ; DESOUZA, ERNANE C. ; HIASA, HIROSHI ; ZHAO, XILIN ; KERNS, ROBERT J. ; DRLICA, KARL . Suppression of gyrase-mediated resistance by C7 aryl fluoroquinolones. Nucleic Acids Research , v. 44, p. gkw161, 2016.

• SOUZA, ERNANE ; FELTON, J. ; CRASS, RYAN L. ; HANAYA, KENGO ; PAI, MANJUNATH P. . Exploratory study of linezolid and linezolid metabolite concentrations in patients with renal impairment. In: American Society of Health-System Pharmacists Midyear Clinical Meeting and Exhibition, 2019, Las Vegas, NV. American Society of Health-System Pharmacists Midyear Clinical Meeting and Exhibition, 2019.

• DEAN, M. ; Gabrielle Costello ; SOUZA, ERNANE ; FELTON, JEREMY ; SZAMOSFALVI, B. ; PAI, AMY BARTON . Characterization of metoprolol and metabolite concentrations pre and post hemodialysis: potential implications for intradialytic hypotension and post dialysis fatigue. In: American Society of Nephrology Kidney Week, 2019, Washington, DC. American Society of Nephrology Kidney Week, 2019.

• SOUZA, ERNANE ; CRASS, RYAN L. ; HE, M. ; MATYEKAS, A. ; HOENERHOFF, M. ; PAI, MANJUNATH P. . Imipenem-cilastatin/relebactam lowers acute kidney injury (AKI) in a preclinical model of vancomycin nephrotoxicity. In: 29th European Congress of Clinical Microbiology & Infectious Diseases, 2019, Amsterdam. 29th European Congress of Clinical Microbiology & Infectious Diseases, 2019.

• DEAN, M. ; Gabrielle Costello ; SOUZA, ERNANE ; FELTON, JEREMY ; SZAMOSFALVI, B. ; PAI, MANJUNATH P. ; PAI, AMY BARTON . Characterization of metoprolol parent and metabolite concentrations pre and post dialysis: implications for intradialytic hypotension and post dialysis fatigue. In: National Kidney Foundation Spring Clinical Meeting, 2019, Boston, MA. National Kidney Foundation Spring Clinical Meeting, 2019.

• GUIMARAES, F. P. G. ; NASCIMENTO, JORGE W.L. ; SOUZA, ERNANE ; MOURAO JUNIOR, C. A. . Adverse reactions of dipyrone and policies for its withdrawal from the market: a narrative review. In: 4th Congress of Pharmaceutical Sciences of Ouro Preto, 2019, Ouro Preto, MG. 4th Congress of Pharmaceutical Sciences of Ouro Preto, 2019.

• SOUZA, ERNANE ; CRASS, RYAN L. ; HE, M. ; MATYEKAS, A. ; HOENERHOFF, M. ; PAI, MANJUNATH P. . Imipenem-cilastatin/relebactam lowers acute kidney injury in a preclinical model of vancomycin nephrotoxicity. In: University of Michigan College of Pharmacy Research Forum, 2019, Ann Arbor, MI. University of Michigan College of Pharmacy Research Forum, 2019.

• PAI, AMY BARTON ; YESSAYAN, LENAR T. ; Gabrielle Costello ; DEAN, M. ; MUKHERJEE, SUSHOVITA ; SOUZA, ERNANE . Intracellular labile iron pool in isolated monocytes from hemodialysis patients is higher with maintenance versus loading dosing of intravenous iron. In: 21st International Conference on Dialysis Advances in Kidney Disease, 2019, New Orleans, LA. 21st International Conference on Dialysis Advances in Kidney Disease, 2019.

• SOUZA, ERNANE ; CRACIUN, I. ; KERNS, ROBERT J. . Novel charge-reduced heparin derivatives revealed as direct allosteric inhibitors of thrombin. In: 255th American Chemical Society National Meeting, 2018, New Orleans, LA. 255th American Chemical Society National Meeting, 2018.

• SOUZA, ERNANE ; CRACIUN, I. ; KULKARNI, C. A. ; KERNS, ROBERT J. . Identification of modified-heparin derivatives as novel direct allosteric inhibitors of thrombin. In: 26th Annual Center for Biocatalysis and Bioprocessing Conference, 2017, Iowa City, IA. 26th Annual Center for Biocatalysis and Bioprocessing Conference, 2017.

• SOUZA, ERNANE ; KERNS, ROBERT J. . Charge-reduced N-desulfonated/N-acylated heparin derivatives: novel direct allosteric inhibitors of thrombin. In: 19th Annual Jakobsen Memorial Conference, 2017, Iowa City, IA. 19th Annual Jakobsen Memorial Conference, 2017.

• SOUZA, ERNANE ; CRACIUN, I. ; KERNS, ROBERT J. . Characterization of charge-reduced N-acylated heparin derivatives as novel direct allosteric inhibitors of thrombin. In: 2017 Health Sciences Research Week, 2017, Iowa City, IA. 2017 Health Sciences Research Week, 2017.

• SOUZA, ERNANE ; CRACIUN, I. ; FERNANDEZ, C. ; KERNS, ROBERT J. . Novel charge-reduced N-acylated heparin derivatives as direct allosteric inhibitors of thrombin. In: 55th Annual MIKI Midwestern Medicinal Chemistry Meeting, 2017, Minnesota, MN. 55th Annual MIKI Midwestern Medicinal Chemistry Meeting, 2017.

• SOUZA, ERNANE ; MALIK, MUHAMMAD ; MUSTAEV, ARKADY ; SCHWANZ, HEIDI A. ; LUAN, GAN ; SHAH, NIRALI ; OPPEGARD, LISA M. ; HIASA, HIROSHI ; ZHAO, XILIN ; DRLICA, KARL ; KERNS, ROBERT J. . Synthesis and evaluation of C7 aryl fluoroquinolones to overcome gyrase-mediated bacterial resistance. In: 54th Annual MIKI Midwestern Medicinal Chemistry Meeting, 2016, Iowa City, IA. 54th Annual MIKI Midwestern Medicinal Chemistry Meeting, 2016.

• SOUZA, ERNANE ; CALVARIO, V. G. ; OLIVO, H. F. . Synthesis of dihydroxyanthracenones and naphthopyranones through Houser and Stauton-Weinreb annulations and oxidative coupling reactions. In: 53th Annual MIKI Midwestern Medicinal Chemistry Meeting, 2015, Lawrence, KS. 53th Annual MIKI Midwestern Medicinal Chemistry Meeting, 2015.

• Nascimeto, J.W.L. ; SOUZA, ERNANE ; PINTO, ALEXANDRE F. ; BONFANTE, HERVAL L. . Evaluation of the hematological alterations after the therapeutic use of dipyrone sodium. In: 17th World Congress of Basic & Clinical Pharmacology, 2014, Cape Town. 17th World Congress of Basic & Clinical Pharmacology, 2014.

• SOUZA, ERNANE ; MATOS, DALYARA M. ; VIANA, MILAINY R. ; ALVIM, MARCELA C.O. ; BONFANTE, HERVAL L. ; PINTO, ALEXANDRE F. ; NASCIMENTO, JORGE W.L. . Evaluation of hematological alterations after therapeutic use of dipyrone in healthy adults: a prospective study. In: 19th Seminar of Scientific Initiation of the Federal University of Juiz de Fora, 2013, Juiz de Fora, MG. 19th Seminar of Scientific Initiation of the Federal University of Juiz de Fora, 2013.

• SOUZA, ERNANE ; PINTO, ALEXANDRE F. ; NASCIMENTO, JORGE W.L. . Evaluation of the leukocyte profile of health volunteers after administration of dipyrone (metamizole). In: 18th Seminar of Scientific Initiation of the Federal University of Juiz de Fora, 2012, Juiz de Fora. 18th Seminar of Scientific Initiation of the Federal University of Juiz de Fora, 2012.

• Willian S. Araujo ; FORTUNATO, I. O. ; SOUZA, ERNANE . Galactokinase: pathologies associated with galactosemia. In: 15th South American Symposium of Farmapolis, 2010, Florianópolis, SC. 15th South American Symposium of Farmapolis, 2010.

• PANQUESTOR, E. K. ; MADALENA, M. ; SOUZA JÚNIOR, E. C. ; OLIVEIRA, E. S. . Definição de Unidade de Paisagem da Bacia do Rio Carangola - MG, a partir da Aplicação do Geoprocessamento. In: IV Encontro de Produção Científica, 2005, Diamantina, MG. Anais do 4 Encontro de Produção Científica, 2005.

• SOUZA, ERNANE . Translational approaches toward optimization of linezolid therapy in patients with renal impairment. 2020. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Accumulation of major linezolid metabolites in patients with renal impairment. 2019. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Characterization of metoprolol during and after hemodialysis: implications for intradialytic hypotension and post dialysis fatigue. 2018. (Apresentação de Trabalho/Simpósio).

• SOUZA, ERNANE . Novel direct allosteric inhibitors of thrombin as potentially new anticoagulants. 2018. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Novel direct allosteric inhibitors of thrombin. 2018. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Novel direct allosteric inhibitors of thrombin as potentially new anticoagulants. 2018. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Novel direct allosteric inhibitors of thrombin. 2018. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . How drugs are made: approaches towards development of a new anticoagulant. 2017. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Allosteric activation of human -thrombin through exosite 2 by suramin analogs. 2017. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Potent, selective, allosteric inhibition of human plasmin by sulfated non-saccharide glycosaminoglycan mimetics. 2017. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Designing allosteric regulators of thrombin: monosulfated benzofuran dimers selectively interact with Arg173 of exosite 2 to induce inhibition. 2016. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Macrocyclic prodrugs of a selective nonpeptidic direct thrombin inhibitor display high permeability, efficient bioconversion but low bioavailability. 2016. (Apresentação de Trabalho/Seminário).

• de Souza, Ernane C. . Development of novel biflavonoids against Trypanosoma cruzi. 2015. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Thiazolidinone-peptide hybrids as dengue virus protease inhibitors with antiviral activity in cell culture. 2015. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Conversion of a highly selective sigma-1 receptor-ligand to sigma-2 receptor preferring ligands with anticocaine activity. 2014. (Apresentação de Trabalho/Seminário).

• SOUZA, ERNANE . Dipyrone (metamizol): clinical, pharmacological and political aspects of its market withdrawal. 2012. (Apresentação de Trabalho/Seminário).

Projetos de pesquisa

• 2019 - 2020

  Development of a Sensitive LC-MS/MS Method for Quantification of Linezolid and Its Primary Metabolites in Human Serum, Descrição: Linezolid (LZD) is a widely used antimicrobial that is active against a broad range of disease-causing bacteria. Myelosuppression is major treatment-limiting toxicity of LZD that occurs more frequently in patients with renal insufficiency. Quantification of LZD and its two primary metabolites (PNU-142300 and PNU-142586), which undergo significant renal elimination, may support design of improved dosing strategies to mitigate the risk of myelosuppression. In this study, we established the first liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of LZD and its main metabolites in human serum. Proteins in serum samples were precipitated with acetonitrile containing a deuterated internal standard. Chromatographic separation of analytes was performed with Waters X-bridge column (C18, 150 4.6 mm, 3.5 m) at 25 °C and subjected to mass analysis using positive electro-spray ionization. The mobile phase A was water with 0.1% formic acid, and mobile phase B was acetonitrile with 0.1% formic acid at a flow rate of 0.6 mL/min, within a 15 min run time. Standard curves were linear and correlation coefficients (r2) were 0.99 for concentration ranges of 0.1-50 g/mL for LZD and PNU-142300, and 0.1-25 g/mL for PNU-142586. The inter- and intra-assay precisions were <15% for all analytes in quality control samples, and the accuracies ranged from 97 to 112%. Extraction recoveries ranged from 78 to 103% for all analytes, and there was no significant matrix effect. Samples from 10 patients (5 with renal impairment) were assayed. Mean (SD) LZD, PNU-142300 and PNU-142586 trough concentrations were 19.4(6.8), 11.6(6.8), 25.7(16.4) g/mL, respectively, in patients with renal impairment. These values were 2.5-, 5.8-, and 6.8-fold higher for LZD, PNU-142300 and PNU-142586, respectively compared to patients without renal impairment. The method was effectively applied in the determination of LZD and its main metabolites in human serum.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Ernane Cláudio de Souza Júnior - Coordenador / Jeremy Felton - Integrante / Manjunath P Pai - Integrante / Ryan L Crass - Integrante / Kengo Hanaya - Integrante.

• 2016 - Atual

  Novel direct allosteric inhibitors of thrombin as potentially new anticoagulants, Descrição: Regulation of thrombin commands a high interest in the medical community because it is a key enzyme involved in blood coagulation, being an important target for preventing thrombotic disorders, such as heart attacks, deep vein thrombosis, pulmonary embolism, and stroke. Heparin, a widely used anticoagulant, catalyzes inhibition of thrombin indirectly by activating antithrombin. However, clinical utility of heparin is plagued by significant adverse reactions, most of which are associated with the chemical nature of heparin itself. Based on the molecular interactions observed in heparin-binding proteins, a library of charge-reduced modified heparin derivatives was screened for compounds having activity towards direct thrombin inhibition. Modified-heparin derivatives possessing 3-(4-hydroxyphenyl)propionate moieties in place of N-sulfo groups were found to be potent, dose-dependent, direct inhibitors of thrombin. Taking these initial findings into consideration, it was postulated that similarly modified N- arylacyl O-sulfonated aminoglycosides would function as low molecular weight structural mimics of heparin. In an effort to N-acylate aminoglycosides, byproducts from the organic reactions were isolated and found to be potent, direct inhibitors of thrombin. Two of these byproducts were characterized to be the first unsulfated small molecules to exhibit allosteric direct inhibition of thrombin. Overall, the results of this study indicate that heparin-binding proteins can be effectively targeted by introducing select N-arylacyl groups into modified-heparin derivatives. Moreover, the first unsulfated small molecules that are allosteric inhibitors of thrombin were discovered and characterized. Together this work will guide further investigations towards the development of novel allosteric inhibitors of thrombin as anticoagulant agents.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Ernane Cláudio de Souza Júnior - Integrante / KERNS, ROBERT J. - Coordenador.

• 2013 - 2014

  Selective Lipase-mediated acetylation of primary alcohols in ethyl acetate, Descrição: There are currently various methods available to carry out the acylation of alcohols. Most of them include the use of acyl chlorides or anhydrides in the presence of tertiary amine bases such as pyridine or triethylamine; Lewis or protonic acids; as well as solid acids as catalysts. Vinyl acetate, acetyl chloride and acetic anhydride can be used as the acetylating agents. Nevertheless, most of these methods employ hazardous reagents and do not exhibit selectivity, acetylating either phenols, primary, secondary or tertiary alcohols. Additionally, the use of a wide range of catalysts demands further separation and purification steps. Therefore, a green replacement for the catalysts and the acetylating agents to lead in a selective reaction is required. In this project, we have reported a practical, safe, inexpensive and green method for obtaining selective acetylation of primary alcohols, using lipase (Novozyme-435) as the catalyst and ethyl acetate as the solvent as well as the acetyl-transfer agent under mild conditions. The cheapness and availability of the reagents, easy procedure and workup make this process a useful and environmentally friendly addition to the present methodologies for the obtainment of selective acetylated primary alcohols.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Ernane Cláudio de Souza Júnior - Integrante / Victor Gomez Calvario - Integrante / Horacio F Olivo - Coordenador.

• 2013 - 2014

  Total synthesis of peroxisomicine A1 and analogs with anti-cancer activity, Descrição: A large problem with cancer chemotherapy is the lack of selectivity of the agents against malignant versus normal cells. Thus, new chemotherapeutic agents with selective cytotoxicity against cancerous cells are highly desirable. Interestingly, Peroxisomicine A1 (PA1), which is a complex dimeric natural product isolated from the seeds of the fruits of different species of Karwinskia, was found to possess significant selective cytotoxicity against lung, liver, and colon tumor cells, displaying high therapeutic index. Biological studies up to date have been performed with limited amounts of the natural product, due to the low availability of PA1 isolated from the seeds of plants. For this reason, we aim to propose an efficient total synthesis of PA1 and simplified analogs in order to provide enough material for further biological studies and determine the structure-activity relationship of these compounds.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Ernane Cláudio de Souza Júnior - Integrante / Victor Gomez Calvario - Integrante / Horacio F Olivo - Coordenador.

• 2011 - 2013

  Avaliação do perfil leucocitário de voluntários após o uso terapêutico de dipirona sódica, Descrição: A dipirona é um dos analgésicos/antipiréticos mais utilizados no Brasil,sendo indicada para cefaléias,neuralgias,dores reumáticas,pós-operatórias,entre outras.Porém,um ponto controverso na sua utilização seria o possível efeito depressor da medula óssea,o que poderia levar a um quadro de anemia aplásica e,principalmente,agranulocitose.Desta forma, devido às controvérsias internacionais quanto ao risco da sua utilização,estudos relacionados ao tema podem auxiliar na definição do custo-benefício do uso terapêutico deste medicamento.O objetivo deste projeto de pesquisa foi estabelecer se existe alguma relação entre a variação do perfil leucocitário de voluntários sadios e a administração de dipirona sódica em doses terapêuticas durante 7 dias consecutivos.Para isso, foi estabelecido um n=50 voluntários de ambos os sexos. Os indivíduos receberam comprimidos de 500 mg de dipirona sódica em quantidade suficiente para utilização 4 vezes ao dia por um período de 7 dias. Inicialmente,foi realizada uma coleta de 3 mL de sangue nos voluntários para avaliação do perfil leucocitário basal, anterior ao tratamento. Decorridos os 7 dias de administração do medicamento,os voluntários foram submetidos a nova coleta de sangue para avaliar o perfil leucocitário.Em seguida, foram determinados os valores da leucometria global, leucometria específica, o eritrograma e a contagem plaquetária.Com base nos resultados obtidos e após análise estatística por test t de student pareado, tendo como indicativo de significância estatística p<0,05, não foi verificada nenhuma diferença significativa quando comparada a contagem leucocitária dos indivíduos antes e após o uso da dipirona. Apesar do pequeno número de voluntários envolvidos neste estudo, os dados obtidos constituem em importantes indicativos do nível de risco e segurança da utilização deste medicamento na clínica.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Ernane Cláudio de Souza Júnior - Integrante / Jorge Wilian L. Nascimento - Coordenador., Financiador(es): Conselho Nacional de Desenvolvimento Científico e Tecnológico - Bolsa.

• 2010 - 2011

  Estudo da composição química do extrato de diclorometano de Rhigozum madagascariensis, Descrição: A malária permanece como uma importante causa de morte no mundo, sobretudo nos países de clima tropical, como o Brasil. Suas atuais formas de tratamento são precárias e inespecíficas, havendo a necessidade do desenvolvimento de novos medicamentos para o combate mais eficaz dessa doença. O objetivo deste trabalho de pesquisa foi investigar a composição química do extrato de diclorometano de Rhigozum madagascariensis , bem como as suas propriedades biológicas sobre diferentes cepas de Plasmodium falciparum in vitro. Metodologia: foram selecionadas diferentes frações obtidas a partir do extrato de diclorometano de Rhigozum madagascariensis por meio de técnicas de cromatografia líquida. Em seguida, essas frações foram analisadas em equipamentos de Espectrometria de Massas (MS), Espectrometria de Infravermelho (IR) e Espectrometria de Ressonância Magnética Nuclear de alta definição (RMN-HD). As diferentes frações obtidas foram submetidas a testes in vitro para a avaliação de suas propriedades biológicas contra cepas de Plasmodium vivax. Resultados: a partir de frações específicas obtidas a partir do extrato de diclorometano de Rhigozum madagascariensis, foram identificados compostos de natureza quinônica, antraquinónica, isoflavônica, antrocianidínica, entre outras. Alguns destes compostos (sobretudos os de natureza quinônica) apresentaram excelentes propriedades inibitórias sobre as cepas de Plasmodium vivax, constituindo-se em potenciais novos fármacos a serem utilizados para o tratamento da malária.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Ernane Cláudio de Souza Júnior - Integrante / Esther del Olmo Fernández - Coordenador.

• 2005 - 2006

  Definição de Unidade de Paisagem da Bacia do Rio Carangola - MG, a partir da Aplicação do Geoprocessamento, Descrição: O projeto de pesquisa consistiu na caracterização do relevo do município de Carangola - MG com o objetivo de definir as unidades geomorfológicas e o uso do solo em ambiente computacional, por meio da utilização das técnicas de geoprocessamento. Para a realização do estudo foram utilizados dados altimétricos contidos em cartas topográficas digitalizadas na escala de 1:50.000 obtidas junto ao IBGE - Instituto Brasileiro de Geografia e Estatística. Estes dados foram interpolados para a elaboração de um Modelo Digital de Terreno relativo a área de estudo. Além disso, foram empregadas imagens de satélite LandSat 5 de domínio público, as quais foram processadas a fim de se fazer a classificação do uso do solo. A partir do modelo gerado foi realizada a caracterização geomorfológica da área segundo unidades ambientais, além da análise do uso do solos de acordo com esses compartimentos.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Ernane Cláudio de Souza Júnior - Integrante / Evandro Klen Panquestor - Coordenador / Maria Madalena - Integrante.