Humberto Ramírez Medina

Humberto studied Biochemistry at the Technological Institute of Celaya in Mexico. His scientific career began during his Master with study on plant-pathogen interaction, with emphasis on genes from Sclerotium cepivorum involved in its pathogenesis on Allium sativum. He then moved to the Laboratory of excellent geneticist Enrique Cerda-Olmedo at the University of Seville in Spain. During his doctoral courses, he studied the effect of light on transcription of genes for carotenogenesis in Phycomyces blakesleeanus. He analyzed the transcription of genes involved in the carotenogenesis and mating in Blakeslea trispora. For his Diploma, he identified supposed genes for the early processing of beta-carotene in Phycomyces. For his PhD, he continued working under supervision of Prof. Cerda-Olmedo with emphasis on carotenoid cleavage oxygenases of Phycomyces. For this, he moved to the Laboratory of Prof. Salim Al Babili at the University of Freiburg in Germany where he corroborated the enzymatic activity of two oxygenases. These results confirmed the cleaving of beta-carotene and elucidated the first steps in the trisporic acids biosynthesis, essential apocarotenoids for sexual cycle in Mucorales. These results decisively rejected the hypothesis assumed during many years for this pathway. His studies on Phycomyces mutants affected in carotenogenesis and sexual reproduction corroborated that beta-carotene and its cleaving to apocarotenoids are essentials for mating in Mucorales and confirmed that CarS protein (one of the oxygenases mentioned) has a role in the carotenogenesis regulation. He also studied the role of polyamines in growth of Phycomyces. He then moved to the Laboratory of Prof. Alain Tissier at the Leibniz Institute of Plant Biochemistry in Germany. He has engineered transgenic plants of Nicotiana tabacum which produce carotenoids in their glandular trichomes by expression of genes for synthesis of these isoprenoids from Phycomyces and other organisms. He taught Molecular Biology to students of Master and co-supervised a Bachelor Thesis at Technological Institute of Celaya. He supervised training at laboratory of a Genetics course at University of Seville.

Informações coletadas do Lattes em 04/11/2022

Acadêmico

Formação acadêmica

Doutorado em Genetics and Technology Microbial

2008 - 2013

Universidad de Sevilla
Título: Biosynthesis of apocarotenoids in Phycomyces Blakesleanus.
Orientador: Prof. Dr. Enrique Cerdá Olmedo
Bolsista do(a): Instituto de Financiamiento e Información para la Educación. Palavras-chave: Phycomyces; Sexual interaction; Trisporic acids; Mutants.Grande área: Ciências Biológicas / Área: Genética. Grande Área: Ciências Biológicas / Área: Bioquímica. Grande Área: Ciências Biológicas / Área: Bioquímica / Subárea: Biologia Molecular.

Mestrado em Genetics and Microbial Technology

2005 - 2007

University of Seville
Título: Genes and metabolites of sexual cycle of Phycomyces blakesleeanus,Ano de Obtenção: 2007
Orientador: Prof. Dr. Enrique Cerdá Olmedo
Bolsista do(a): Instituto de Financiamiento e Información para la Educación. Palavras-chave: Sexual interaction; Trisporic acids.Grande área: Ciências Biológicas / Área: Biologia Geral / Subárea: MOLUCULAR BIOLOGY.

Mestrado em Biochemical Engineering

2001 - 2003

Instituto Tecnológico de Celaya
Título: Construction of Subtractive library containing gene fragments involved at the Sclerotium cepivorum pathogenesis on garlic (Allium sativum),Ano de Obtenção: 2003
Orientador: Prof. Dr. Lorenzo Guevara Olvera
Bolsista do(a): Consejo del Sistema Nacional de Educación Tecnológica. Palavras-chave: Sclerotium cepivorum; White rot; suppression subtractive hybridization.Grande área: Ciências Biológicas / Área: Biologia Geral / Subárea: MOLUCULAR BIOLOGY.

Graduação em Biochemical Engineer

1994 - 2000

Instituto Tecnológico de Celaya
Título: Molecular and physicochemical analysis of maize (Zea mays)
Orientador: Dr. Lorenzo Guevara Olvera and Dr. José Luis Pons Hernández.

Pós-doutorado

2013 - 2014

Pós-Doutorado. , Leibniz-Institut für Pflanzenbiochemie. , Bolsista do(a): Consejo Nacional de Ciencia y Tecnología. , Grande área: Ciências Biológicas / Área: Biologia Geral / Subárea: MOLUCULAR BIOLOGY.

Idiomas

Inglês

Compreende Bem, Fala Razoavelmente, Lê Bem, Escreve Razoavelmente.

Áreas de atuação

Grande área: Ciências Biológicas / Área: Biologia Geral / Subárea: MOLUCULAR BIOLOGY.

Grande área: Ciências Biológicas / Área: Bioquímica.

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: Enzimologia.

Grande área: Ciências Biológicas / Área: Microbiologia / Subárea: Biologia e Fisiologia dos Microorganismos/Especialidade: Micologia.

Participação em eventos

22nd Conference on Isoprenoids. Carotenoids synthesis in Nicotiana trichomes. 2014. (Congresso).

The European Molecular Biology Organization Meeting. Apocarotenoids in the sexual development of Phycomyces. 2012. (Congresso).

The Cell Signaling Networks Conference. Phycomyces mutants lacking apocarotenoids. 2011. (Congresso).

The XXXVIIIth Congress of the Spanish Society of Genetics. Phycomyces mutants lacking apocarotenoids. 2011. (Congresso).

The Meeting of the Spanish Network of Filamentous Fungi Researchers.Apocarotenoids biosynthesis in Phycomyces blakesleeanus. 2010. (Encontro).

The Xth International Fungal Biology Conference & The VIIIth National Congress of Cellular and Molecular Biology of Fungi. Apocarotenoids biosynthesis in Phycomyces blakesleeanus. 2009. (Congresso).

The XXXVIIth Congress of the Spanish Society of Genetics. A beta-carotene oxygenase, the product of gene carS, is the main regulator of beta-carotene synthesis in the fungus Phycomyces blakesleeanus. 2009. (Congresso).

Max Delbrück and the New perception of Biology. A Centennial Celebration. 2006. (Simpósio).

The Meeting of the Molecular Genetics Network of Filamentous Fungi..Gene involved at the Sclerotium cepivorum pathogenesis on garlic. 2006. (Encontro).

The V National Congress of Cellular and Molecular Biology of Fungi. Gene detection of Sclerotium cepivorum during its pathogenesis on garlic (Allium sativum) using SSH and cDNA microarrays. 2003. (Congresso).

The Pan American Plant Disease Conference. Gene Identification of Sclerotium cepivorum during its pathogenesis on garlic (Allium sativum) using SSH and cDNA microarrays.. 2003. (Congresso).

The XV Biochemical Engineering.Construcción de un banco substractivo conteniendo fragmentos de genes involucrados en la patogénesis de Sclerotium cepivorum en Ajo (Allium sativum). 2003. (Oficina).

The VIII International Fungal Biology Conference. 2002. (Congresso).

The II International Congress on foods Innocuousness. 2000. (Congresso).

Participação em bancas

Aluno: María Isabel Hernández Álvarez

Hernández-Álvarez, M.I.; Guevara-Olvera, L.;RAMÍREZ-MEDINA, HUMBERTO. Construcción de un banco de ADNc de Sclerotium cepivorum enpresencia de ajo (Allium sativum). 2003. Trabalho de Conclusão de Curso (Graduação em Biochemical Engineer) - Instituto Tecnológico de Celaya.

Produções bibliográficas

TAGUA, V. G. ; MEDINA, H. R. ; Martín-Domínguez, R. ; ESLAVA, A.P. ; Corrochano, L.M. ; Cerdá-Olmedo, E. ; Idnurm, A. . A gene for carotene cleavage required for pheromone biosynthesis and carotene regulation in the fungus Phycomyces blakesleeanus. Fungal Genetics and Biology (Print) , v. 49, p. 398-404, 2012.
MEDINA, H. R. ; Acosta-García, G. ; Muñoz-Sánchez, C. I. ; Hernández-Álvarez, M.I. ; González-Chavira, M.M. ; Delgadillo-Sánchez, F. ; Torres-Pacheco, I. ; GONZALEZ, R. G. G. ; Guevara-Olvera, L. . Profiling the transcriptome of Sclerotium cepivorum Berk related to white rot on garlic (Allium sativum Linnaeus). African Journal of Microbiology Research , v. 6, p. 2752-2760, 2012.
Medina, Humberto R. ; CERDÁ-OLMEDO, ENRIQUE ; AL-BABILI, SALIM . Cleavage oxygenases for the biosynthesis of trisporoids and other apocarotenoids in Phycomyces. Molecular Microbiology (Print) , v. 82, p. 199-208, 2011.
KUZINA, VERA ; RAMÍREZ-MEDINA, HUMBERTO ; VISSER, HANS ; OOYEN, ALBERT J. J. ; CERDÁ-OLMEDO, ENRIQUE ; BERG, JOHAN A. . Genes involved in carotene synthesis and mating in Blakeslea trispora. Current Genetics , v. 54, p. 143-152, 2008.
Guevara-Olvera, L. ; Hernández-Álvarez, M.I. ; RAMÍREZ-MEDINA, HUMBERTO ; Magaña-Vázquez, J.J. ; Delgadillo-Sánchez, F. ; Guevara-Gozález, R.G. . Combining SSH and cDNA microarrays for rapid identification of ESTs associated with garlic white rot. In: Guevara González, R.G. and Torres-Pacheco, I.. (Org.). In Advances in Agricultural and Food Biotechnology. 661ed.Kerala: Research Signpost, 2006, v. 37, p. 55-69.
HUMBERTO MEDINA ; SYLVESTRE MARILLONNET ; GERD BALCKE ; ALAIN TISSIER . Carotenoids synthesis in Nicotiana trichomes. In: 22nd Conference on Isoprenoids, 2014, Prague. Chemické Listy S. Prague: Czech Chemical Society, 2014. v. 108. p. 89-150.
RAMÍREZ-MEDINA, HUMBERTO ; Muñoz-Sánchez, C. I. ; Guevara-Gozález, R.G. ; Guevara-Olvera, L. . Identificación y clonación de genes involucrados en la patogénesis de Sclerotium cepivorum Berk. en ajo (Allium sativum L.) empleando hibridaciones substractivas y microarreglos de ADNc. In: 17ma conferencia de Química., 2002, Santiago de Cuba. Memorias de la 17ma conferencia de Química, 2002.
Alcalde, E. ; MEDINA, H. R. ; M. Mar Herrador ; BARRERO, A. F. ; Cerdá-Olmedo, E. . Cyclofarnesoids and methylhexanoids produced from β-carotene in Phycomyces. Organic & Biomolecular Chemistry , 2015.

Outras produções

MEDINA, H. R. ; ALAIN TISSIER . Activities report. 2014. (Relatório de pesquisa).

Lopez-Calderon, I. ; Ramos-Morales, F. ; Wellinger, R.E. ; MEDINA, H. R. . Laboratory training on basic protocols of Molecular Biology. 2007. (Curso de curta duração ministrado/Outra).

Guevara-Olvera, L. ; MEDINA, H. R. . An introduction to Biotechnology. 2003. (Curso de curta duração ministrado/Outra).

Guevara-Olvera, L. ; MEDINA, H. R. . Selected topics of Biotechnology. 2003. (Curso de curta duração ministrado/Outra).

Guevara-Olvera, L. ; MEDINA, H. R. . Selected topics of Biotechnology. 2002. (Curso de curta duração ministrado/Outra).

Projetos de pesquisa

2010 - 2012

Synthesis and function of xanthophylls, apocarotenoids and gibberellins in fungi, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Humberto Ramírez Medina - Integrante / Humberto Ramirez Medina - Integrante / CERDÁ-OLMEDO, ENRIQUE - Integrante / Francisco Javier Ávalos Cordero - Coordenador / Eugenio Alcalde Rodriguez - Integrante / Marta Castrillo Jiménez - Integrante / Jorge García Martínez - Integrante / Violeta Díaz Sánchez - Integrante / Alejandro Fernández Estrada - Integrante / Luis Roberto Rodríguez Ortiz - Integrante., Financiador(es): Ministerio de Ciencia e Innovación - Auxílio financeiro.
2009 - 2014

Physiological and chemical regulations of carotenes synthesis in Zygomycetes, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Humberto Ramírez Medina - Integrante / Humberto Ramirez Medina - Integrante / CERDÁ-OLMEDO, ENRIQUE - Coordenador / Eugenio Alcalde Rodriguez - Integrante / Maria Del Mar Herrador Del Pino - Integrante / Juan Sánchez García - Integrante / Dolores Elvira Pérez de Camino Cantos - Integrante / Silvia Polaino Orts - Integrante., Financiador(es): Junta de Andalucía - Auxílio financeiro.
2001 - 2003

Identification and cloning of genes involved in Sclerotium cepivorum pathogenesis on garlic (Allium sativum) using suppression subtractive hibridization and microarrays of cDNA, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Humberto Ramírez Medina - Integrante / Humberto Ramirez Medina - Integrante / Lorenzo Guevara Olvera - Coordenador., Financiador(es): Consejo Nacional de Ciencia y Tecnología - Auxílio financeiro., Número de produções C, T & A: 8

Prêmios

2015

Member of National System of Researchers of Mexico, National Council for Science and Technology of Mexico.

2013

cum laude for doctoral dissertation, University of Seville.

Histórico profissional

Experiência profissional

2010 - 2011

Universidad de Sevilla

Vínculo: Colaborator, Enquadramento Funcional: Technician

Outras informações:
Project: Producción industrial de giberelinas GA1 Y GA3 mediante fermentaciones de estirpes silvestres del hongo Gibberella fujikuroi. Reference: 0544/0289. Financial support: Bioflow Sur, S.L. Principal Researcher: Francisco Javier Ávalos Cordero.

2006 - 2009

Universidad de Sevilla

Vínculo: Colaborator, Enquadramento Funcional: Technician

Outras informações:
Project: Química, Genética y Biotecnología del ciclo sexual de los Cigomicetos. Reference: EXC/2005/CVI-910. Principal Researcher: Enrique Cerdá Olmedo. Financial support: Junta de Andalucía (Plan Andaluz de Investigación).

2003 - 2003

Instituto Tecnológico de Celaya

Vínculo: Colaborator, Enquadramento Funcional: Technician

Outras informações:
Project: Identification and cloning of genes involved in Sclerotium cepivorum pathogenesis on garlic (Allium sativum) using suppression subtractive hibridization and microarrays of cDNA. Reference: 37546-3. Financial support: Consejo Nacional de Ciencia y Tecnología , Mexico. Principal Researcher: Prof. Dr. Lorenzo Guevara Olvera.

1999 - 2000

La Mesa S.A. de C.V.

Vínculo: , Enquadramento Funcional: Manufacturing Process Manager, Regime: Dedicação exclusiva.

1996 - 1999

Telepizza México S.A. de C.V

Vínculo: , Enquadramento Funcional: Manager to drive-thru restaurant, Regime: Dedicação exclusiva.