Iris Mangas Nadal

Young Talent researcher at Instituto Militar de Engenheria, IME, Rio de Janeiro, September 2014-August 2017, Bolsista Jovem Talento, Level A. Previous: 5 years Bachelors degree in Toxicology-Pharmacology - Universidad Miguel Hernández, Spain (2008); Master degree in Enviromental Toxicology- Universidad de Valencia, Spain (2010); Master degree in Public Health- Universidad Miguel Hernández, Spain (2008) ph D international mention degree in Bioingeniering in the Toxicology and Chemical Safety Unit of Universidad Miguel Hernández- University of California-San Diego (January 2014). My research was devoted in mechanistic aspects of organophosphorus compounds toxicity and detoxication routes. The focus of my ph D research was to study the molecular interaction of nervous system enzymes (esterases) interacting with organophosphorus compounds. Part of the research was done in the University of California-San Diego and I awarded an international mention degree. During my ph D I have been working in the kinetic characterization(inhibition, reactivation) of the binding of esterases in order to identify target proteins of organophosphorus toxicity and/or detoxification. This kinetic characterization was used to have criteria for monitoring during isolation and purification studies using separation methods, mainly by HPLC. Tandem mass spectrometry (LC-MS-MS) in the AB SCIEX TripleTOF 5600 System followed by bioinformatic approaches were used to identify molecularly some of these proteins. Peptide mass fingerprinting by mass spectrometry was used to resolve the structures of the inhibited enzyme and of the post-inhibitory modification pathways in purify enzymes. I also have experience in microbiology and DNA, RNA molecular biology methods as I was working 9 months during my undergraduate studies.

Informações coletadas do Lattes em 04/11/2022

Acadêmico

Formação acadêmica

Doutorado em pharmacology

2009 - 2014

Universidad Miguel Hernandez
Título: Brain carboxylesterases interacting with organophosphorus compounds: kinetic characterization and approaches to purification and molecular identification
Orientador: EUGENIO VILANOVA GISBERT
com Coorientador: JORGE ESTÉVEZ DOMENECH. Bolsista do(a): INSTITUTO NACIONAL DE INVESTIGACIONES AGRARIAS.

Mestrado profissional em POLLUTION AND ENVIROMENTALTOXICOLOGY MASTER DEGRE

2008 - 2010

Universitat de València
Título: Kinetic interaction of soluble brain carboxilesterases with organophosphorus compounds, Ano de Obtenção: 2010
Orientador: EUGENIO VILANOVA GISBERT
Coorientador: JORGE ESTÉVEZ DOMENECH. Bolsista do(a): MINISTERIO DE CIENCIA Y EDUCACIÓN. Palavras-chave: ORGANOPHOSPHORUS COMPOUNDS; KINETICS; SERINE ESTERASES; NEUROTOXICITY; MECHANISTIC TOXICOLOGY.Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: MOLECULAR BIOLOGY. Grande Área: Ciências da Saúde / Área: Farmácia / Subárea: TOXICOLOGY. Grande Área: Ciências da Saúde / Área: Farmácia / Subárea: Enzimologia.

Especialização em PUBLIC HEALTH MAS TER DEGREE

2010 - 2011

MIGUEL HERNÁNDEZ UNIVERSITY
Título: EFECTOS TÓXICOS DE LA EXPOSICIÓN LABORAL A BAJAS DOSIS DE PLAGUICIDAS ORGANOFOSFORADOS
Orientador: Jesús Vioque
Bolsista do(a): MINISTERIO DE CIENCIA Y EDUCACIÓN.

Graduação em PHARMACY 5 YEARS BACHELORS DEGREE

2002 - 2008

MIGUEL HERNÁNDEZ UNIVERSITY

Pós-doutorado

2014

Pós-Doutorado. , MIGUEL HERNÁNDEZ UNIVERSITY. , Bolsista do(a): EUROPEAN COMMISION. , Grande área: Ciências da Saúde / Área: Saúde Coletiva / Subárea: Medicina Preventiva.

2014

Pós-Doutorado. , Instituto Militar de Engenharia, IME, Brasil. , Bolsista do(a): Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. , Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: BIOQUIMICA. , Grande Área: Ciências Exatas e da Terra / Área: Química / Subárea: Química Orgânica / Especialidade: Química dos Produtos Naturais. , Grande Área: Ciências da Saúde / Área: Farmácia / Subárea: Enzimologia.

Formação complementar

2013 - 2013

Certified Training Voyager DE-STR MASS SPECTROMETR. (Carga horária: 100h). , UNIVERSITY OF CALIFORNIA-SAN DIEGO.

2013 - 2013

Certified Training ABI 4800 MALDI TOF/TOF. (Carga horária: 100h). , UNIVERSITY OF CALIFORNIA-SAN DIEGO.

2011 - 2012

Extensão universitária em COOPERATION AND DEVELOPMENTAL PROJECTS IN PHARMACY. (Carga horária: 500h). , Pharmacist without borders.

2011 - 2011

BASIC PRINCIPLES OF HUMAN RISK ASSESSMENT. (Carga horária: 10h). , SOCIETY OF TOXICOLOGY.

2010 - 2010

Extensão universitária em EVALUATION OF DRUGS WITHOUT ANIMALS. (Carga horária: 10h). , SOCIETY OF TOXICOLOGY.

2010 - 2010

Extensão universitária em  TOOLS AND TECHNOLOGIES IN TRANSLATIONAL TOXICOLO. (Carga horária: 10h). , EUROPEAN SOCIETY OF TOXICOLOGY.

Idiomas

Inglês

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Espanhol

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Português

Compreende Bem, Fala Razoavelmente, Lê Bem, Escreve Razoavelmente.

Italiano

Compreende Bem, Fala Bem, Lê Bem, Escreve Razoavelmente.

Áreas de atuação

Grande área: Ciências da Saúde / Área: Farmácia / Subárea: Análise Toxicológica.

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: BIOQUIMICA.

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: MOLECULAR BIOLOGY.

Grande área: Ciências da Saúde / Área: Saúde Coletiva / Subárea: CHEMICAL SAFETY.

Grande área: Ciências da Saúde / Área: Saúde Coletiva / Subárea: PUBLIC HEALTH.

Grande área: Ciências da Saúde / Área: Saúde Coletiva / Subárea: Farmacologia Bioquímica e Molecular.

Participação em eventos

Research seminars Le laboratoire Architecture et Fonction des Macromolécules Biologiques.Brain carboxilesterases that interact with organophosphates. 2014. (Seminário).

International Congress of Toxicology, (ICT XV). Embryotoxicity Using Gene Expression as Biomarker of Alterations in Differentiation after only 5 Days of Exposure in mouse In Vitro Embryonic Stem Cells. 2013. (Congresso).

38th Federation of European Biochemical Societies (FEBS). Theoretical kinetic aspects of enzymes reacting with OPs.. 2013. (Congresso).

38th Federation of European Biochemical Societies (FEBS). Model equations of inhibition of esterases by non-stable compounds: PMSF as a model. 2013. (Congresso).

SEMINARIOS INTERDISCIPLINARIOS Facultad de ciencias químicas e Ingeniería, Universidad Autonoma de Baja California,.Kinetic interactions of brain carboxilesterases and organophosphorus pesticides, new targets of neurotoxicity?. 2013. (Seminário).

EN LAS FRONTERAS DEL CONOCIMIENTO.Brain carboxilesterases interacting with organophosphorus compounds, new targets of neurotoxicity?. 2013. (Seminário).

52th Annual Meeting of the Society of Toxicology. Kinetic interaction of PMSF (a neuropathy promoter) with membrane bound esterases which are sensitive or resistant to organophosphorus inducers of delayed neuropathy. 2012. (Congresso).

11th International Meeting on Cholinesterases. PMSF alters the interaction of chicken brain esterases with Organophosphorous compounds. Type of participation: Poster. 2012. (Congresso).

11th International Meeting on Cholinesterases. Differential interactions of neuropathy inducers, non-inducers and promoters With soluble and membrane esterases: kinetic approaches.. 2012. (Congresso).

International Congress of Toxicology (ICT XIV). Kinetic identification of organophosphate-sensitive esterases in brain membrane.. 2012. (Congresso).

53th Annual Meeting of the Society of Toxicology. Interactions of a promoter of neuropathy and esterases of membrane and soluble fractions of brain. 2012. (Congresso).

14th International Neurotoxicology Association Meeting. Esterases interacting with organophosphorus compounds different to cholinesterases and neuropathy target esterase in nerve tissues. 2012. (Congresso).

International Congress of Toxicology (ICT XIV). Inhibition and spontaneous reactivation of esterases by organophosphorus compounds in presence of the substrate: paraoxon as a model.. 2011. (Congresso).

50th Annual Meeting of the Society of Toxicology. Kinetic interaction of soluble esterases of brain and Phenylmethylsulphonyl fluoride (PMSF), an inhibitor protecting and potentiating the organophosphorus delayed neurotoxicity. 2011. (Congresso).

International Congress of Toxicology (ICT XIII). Kinetic caracterization of organophosphorus compounds in esterases of brain membrane. 2011. (Congresso).

Produções bibliográficas

MANGAS, I. ; TAYLOR, P. ; VILANOVA, E. ; ESTÉVEZ, J. ; FRANÇA, T. C. C. ; KOMIVES, E. ; RADI', Z. . Resolving pathways of interaction of mipafox and a sarin analog with human acetylcholinesterase by kinetics, mass spectrometry and molecular modeling approaches. Archives of Toxicology , v. 15, p. 458, 2015.
ESTEVEZ, J. ; VILANOVA, E. ; BENEBENT, M. ; MANGAS, I. ; SOGORB, M. . A Fraction of the Organophosphorus-Sensitive Phenylvalerate Esterase Activity Shows Acetylcholine Hydrolyzing Activity in Soluble Fraction of Chicken Brain. Toxicological Sciences (Print) , v. 144, p. 458, 2015.
MANGAS, I. ; TAYLOR P ; RADICK Z ; VILANOVA, E. ; ESTÉVEZ, JORGE . LC-MS/MS Proteomics Analysis Identifies Butyrylcholinesterase As a Protein with Phenylvalerate Esterase Activity.. Toxicological Sciences (Print) , v. 144, p. 158, 2015.
2014 BENABENT, MÓNICA ; VILANOVA, EUGENIO ; MANGAS, IRIS ; SOGORB, MIGUEL ÁNGEL ; ESTÉVEZ, JORGE . Interaction between substrates suggests a relationship between organophosphorus-sensitive phenylvalerate- and acetylcholine-hydrolyzing activities in chicken brain. Toxicology Letters , v. Feb 24, p. pii: S0378-4274, 2014.
2014 MANGAS, I. ; VILANOVA, E. ; BENABENT, M. ; ESTÉVEZ, J. . Separating esterase targets of organophosphorus compounds in the brain by preparative chromatography. Toxicology Letters , v. 225, p. 167-176, 2014.
2014 MANGAS, IRIS ; VILANOVA, EUGENIO ; ESTÉVEZ, JORGE . Kinetic interactions of a neuropathy potentiator (phenylmethylsulfonyl fluoride) with the neuropathy target esterase and other membrane bound esterases. Archives of Toxicology , v. 88, p. 355-366, 2014.
2013 ESTÉVEZ, JORGE ; MANGAS, IRIS ; SOGORB, MIGUEL ÁNGEL ; VILANOVA, EUGENIO . Interactions of neuropathy inducers and potentiators/promoters with soluble esterases. Chemico-Biological Interactions (Print) , v. 203, p. 245-250, 2013.
MANGAS, I. ; ESTÉVEZ, J. ; VILANOVA, EUGENIO . Interactions of a Promoter/Potentiator of Neuropathy and Esterases of Membrane and Soluble Fractions of Brain. Toxicological Sciences (Print) , v. 132, p. 303, 2013.
2012 MANGAS, IRIS ; VILANOVA, EUGENIO ; ESTÉVEZ, JORGE . Phenylmethylsulfonyl Fluoride, a Potentiator of Neuropathy, Alters the Interaction of Organophosphorus Compounds with Soluble Brain Esterases. Chemical Research in Toxicology , v. 25, p. 2393-2401, 2012.
2012 MANGAS, IRIS ; VILANOVA, EUGENIO ; ESTÉVEZ, JORGE . NTE and non-NTE esterases in brain membrane: Kinetic characterization with organophosphates. Toxicology (Amsterdam) , v. 297, p. 17-25, 2012.
VILANOVA, EUGENIO ; MANGAS, IRIS ; ESTÉVEZ, JORGE . Kinetic identification of organophosphate-sensitive esterases in brain membrane. Toxicology Letters , v. 211, p. S171, 2012.
VILANOVA, E. ; MANGAS, I. ; ESTÉVEZ, JORGE . Kinetic interaction of PMSF (a neuropathy promoter) with membrane-bound esterases that are sensitive or resistant to organophosphorus inducers of delayed neuropathy.. Toxicological Sciences (Print) , v. 126, p. 304, 2012.
2011 MANGAS, IRIS ; VILANOVA, EUGENIO ; ESTÉVEZ, JORGE . Kinetics of the inhibitory interaction of organophosphorus neuropathy inducers and non-inducers in soluble esterases in the avian nervous system. Toxicology and Applied Pharmacology , v. 256, p. 360-368, 2011.
MANGAS, I. ; VILANOVA, E. ; ESTÉVEZ, J. . Kinetic characterization of organophosphorus compounds in esterases of brain membrane. Toxicology Letters , v. 205, p. S231, 2011.
VILANOVA, E. ; MANGAS, I. ; ESTÉVEZ, JORGE . Kinetic interaction of soluble esterases of brain and phenylmethylsulphonyl fluoride (PMSF), an inhibitor protecting and potentiating the organophosphorus delayed neurotoxicity. Toxicological Sciences (Print) , v. 120, p. 2011, 2011.
ESTÉVEZ, J. ; MANGAS, I. ; VILANOVA, E. . Inhibition and spontaneous reactivation of esterases by organophosphorus compounds in presence of the substrate: Paraoxon as a model. Toxicology Letters , v. 196, p. S320-S321, 2010.
MANGAS, I. ; SOGORB, MIGUEL ÁNGEL ; VILANOVA, E. . Oil lubricating (Wexler Ed.).. Encyclopedia of Toxicology, 3rd edition.. 3ed.: , 2014, v. , p. 256-.
MANGAS, I. ; SOGORB, MIGUEL ÁNGEL ; VILANOVA, E. . Petroleum distillates (Wexler Ed.). Encyclopedia of Toxicology, 3rd edition.. 3ed.: , 2014, v. , p. 358-.
MANGAS, I. ; SOGORB, MIGUEL ÁNGEL ; VILANOVA, E. . Pyrene (Wexler Ed.).. Encyclopedia of Toxicology, 3rd edition.. 3ed.: , 2014, v. , p. 558-.
MANGAS, I. ; ESTÉVEZ, JORGE ; VILANOVA, E. . Interactions of a Promoter/Potentiator of Neuropathy and Esterases of Membrane and Soluble Fractions of Brain. In: 52TH SOCIETY OF TOXICOLOGY MEETING, 2013, SAN ANTONIO, TX. THE TOXICOLOGIST, 2013. v. 123. p. 303-304.
VILANOVA, E. ; MANGAS I ; SOGORB, MIGUEL ÁNGEL ; ESTÉVEZ, JORGE . Model equations of inhibition of esterases by non-stable compounds: PMSF as a model. In: 38th Federation of European Biochemical Societies (FEBS), 2013, St. Petersburg, RUSSIA. The FEBS journal. St. Petersburg, RUSSIA, 2013. v. 280. p. 165.
SOGORB, MIGUEL ÁNGEL ; ESTEVAN, C. ; PAMIES, D. ; MANGAS, I. ; ESTÉVEZ, JORGE ; VILANOVA, E. . Predicting Embryotoxicity Using Gene Expression as Biomarker of Alterations in Differentiation after only 5 Days of Exposure in mouse in Vitro Embryonic Stem Cells. In: THE XIII INTERNATIONAL CONGRESS OF TOXICOLOGY, 2013, SEOUL, COREA. THE XIII INTERNATIONAL CONGRESS OF TOXICOLOGY, 2013.
MANGAS, I. ; ESTÉVEZ, JORGE ; VILANOVA, E. . Theoretical kinetic aspects of enzymes reacting with OPs.. In: 38th Federation of European Biochemical Societies (FEBS), 2013, St. Petersburg, RUSSIA. The FEBS journal.
ESTÉVEZ, J. ; MANGAS, I. ; SOGORB, MIGUEL ÁNGEL ; VILANOVA, E. . Esterases interacting with organophosphorus compounds different to cholinesterases and neuropathy target esterase in nerve tissues. In: 14th International Neurotoxicology Association Meeting, 2013, Egmond aan Zeen, The Netherlan. 14th International Neurotoxicology Association Meeting.
MANGAS, I. ; ESTÉVEZ, JORGE ; VILANOVA, E. . PMSF ALTERS THE INTERACTION OF CHICKEN BRAIN ESTERASES WITH ORGANOPHOSPHOROUS COMPOUNDS. In: 11th International Meeting on Cholinesterases, 2012, KAZAN, RUSSIA. 11th International Meeting on Cholinesterases BOOK, 2012.
ESTÉVEZ, JORGE ; MANGAS, I. ; SOGORB, MIGUEL ÁNGEL ; VILANOVA, E. . DIFFERENTIAL INTERACTIONS OF NEUROPATHY INDUCERS, NON-INDUCERS AND PROMOTERS WITH SOLUBLE AND MEMBRANE ESTERASES: KINETIC APPROACHES. In: 11th International Meeting on Cholinesterases, 2012, KAZAN, RUSSIA. 11th International Meeting on Cholinesterases BOOK, 2012.
VILANOVA, EUGENIO ; MANGAS, IRIS ; ESTÉVEZ, JORGE . Kinetic interaction of PMSF (a neuropathy promoter) with membrane bound esterases which are sensitive or resistant to organophosphorus inducers of delayed neuropathy. In: 52th Annual Meeting of the Society of Toxicology, 2012, SAN FRANCISCO. 52th Annual Meeting of the Society of Toxicology.
MANGAS, I. ; ESTÉVEZ, JORGE ; VILANOVA, EUGENIO . Kinetic identification of organophosphate-sensitive esterases in brain membrane.. In: International Congress of Toxicology (ICT XIV),, 2012, Stockholm, Sweden. International Congress of Toxicology (ICT XIV),.
VILANOVA, EUGENIO ; MANGAS, I. ; ESTÉVEZ, JORGE . KINETIC INTERACTION OF SOLUBLE ESTERASES OF BRAIN AND PHENYLMETHYLSULPHONYL FLUORIDE (PMSF), AN INHIBITOR PROTECTING AND POTENTIATING THE ORGANOPHOSPHORUS DELAYED NEUROTOXICITY. In: 50th Annual Meeting of the Society of Toxicology, 2011, Washington DC, USA. 50th Annual Meeting of the Society of Toxicology.
MANGAS I ; ESTÉVEZ, J. ; VILANOVA, EUGENIO . Kinetic caracterization of organophosphorus compounds in esterases of brain membrane. In: International Congress of Toxicology (ICT XIII),, 2011, PARIS. International Congress of Toxicology (ICT XIII),, 2012.
ESTÉVEZ, JORGE ; MANGAS, I. ; VILANOVA, E. . Inhibition and spontaneous reactivation of esterases by organophosphorus compounds in presence of the substrate: paraoxon as a model.. In: : XII International Congress of Toxicology (ICT XII),, 2010, Barcelona. XII International Congress of Toxicology (ICT XII), 2010.
MANGAS, I. . Plague: Historical Aspects and Chemotherapy. military medical science letters , 2014.
MANGAS, I. . Brain carboxilesterases interacting with organophosphorus compounds, new targets of neurotoxicity?. 2014. (Apresentação de Trabalho/Seminário).
MANGAS, I. . Kinetic interactions of brain carboxilesterases and organophosphorus pesticides, new targets of neurotoxicity?. 2013. (Apresentação de Trabalho/Seminário).
MANGAS, IRIS . Brain carboxilesterases than interact with organophosphates. 2013. (Apresentação de Trabalho/Conferência ou palestra).

Projetos de pesquisa

2014 - Atual

FORMAÇÃO DE RECURSOS HUMANOS E DESENVOLVIMENTO DE NOVOS FÁRMACOS CONTRA AGENTES NEUROTÓXICOS E DOENCA DE ALZHEIMER, Descrição: O projeto envolve duas Instituições, corpos docente e discente, com experiências nas áreas propostas: O Programa de Pós-Graduação em Química da Seção de Engenharia do Instituto Militar de Engenharia e o Programa de Pós-graduação em Química da UFRJ e foca no fortalecimento da capacitação na formação de recursos humanos para resolução de problemas associados às questões de defesa, fármacos, saúde pública e ciências de alimentos no Brasil.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (3) / Mestrado acadêmico: (1) . , Integrantes: Iris Mangas Nadal - Integrante / angelo da cunha - Integrante / tanos franca - Coordenador.
2011 - 2013

COLABORATION AGREEMENT FOR THE RISK ASSESSMENT OF BIOCIDES WITH EUROPEAN COMISION, Descrição: Convenio de colaboración para el desarrollo de actividades relativas a la evaluación del riesgo de las sustancias biocidas adjudicadas a España en el reglamento (CE) Nº 1451/2007 de la Comisión. INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Mestrado acadêmico: (2) Doutorado: (2) . , Integrantes: Iris Mangas Nadal - Integrante / SOGORB, MIGUEL ÁNGEL - Integrante / VILANOVA, E. - Coordenador / ESTÉVEZ, J. - Integrante.

Prêmios

2010

Prize awarded by the University of Valencia as Best Master s degree project. Titol: Kinetic interaction of soluble brain carboxilesterases with organophosphorus compounds ., University of Valencia.

2008

Five-Star Student Diploma, as B.Sc. graduate. Miguel Hernández University (UMH). Merit: Academic performance, professional experience, professional skills, curriculum, projection and social proje, Miguel Hernandez University, Spain.

Histórico profissional

Experiência profissional

2014 - Atual

Instituto Militar de Engenharia

Vínculo: , Enquadramento Funcional:

2012 - 2013

UNIVERSITY OF CALIFORNIA-SAN DIEGO

Vínculo: Bolsista, Enquadramento Funcional: VISITOR RESEARCHER, Carga horária: 40, Regime: Dedicação exclusiva.

Outras informações:
As a part of my ph D program I have done a 6 months research stay as a visitor graduate in the Skaggs School of Pharmacy and Pharmaceutical science in University of California- San Diego. Where the work implied was kinetic studies of enzymes than interact with organophosphates and proteomic methods. I was training in the study of proteins by computational Biology methods. Institution: University of California-San Diego Institute: Skaggs School of Pharmacy and Pharmaceutical Science Department: Pharmacology Address: 9500 Gilman Dr., MC 0657La Jolla, CA 92093-0657 Advisors: Professor. Palmer Taylor and Ph D. Zoran Radic In short, the work implicated was: Protein digestion into peptides with Trypsin Binding pathways study (peptide mass fingerprinting) in purify proteins and peptide sequencing by mass spectrometry Training in the proteomics facility of UCSD in Matrix Assisted Laser Desorption /Ionization- Time Of Flight (MALDI-TOF) mass spectrometry: equipments Voyager DE-STR and ABI 4800 MALDI TOF/TOF Large scale protein identification in complex mixtures of proteins, equipment: AB SCIEX TripleTOF 5600 Kinetic data analysis, protein chromatography analysis, proteomics data analysis Use of molecular biology software to study the structure of proteins

2014 - Atual

MIGUEL HERNÁNDEZ UNIVERSITY

Vínculo: Colaborador, Enquadramento Funcional: POSDOCTORAL RESEARCHER, Carga horária: 40, Regime: Dedicação exclusiva.

Outras informações:
Posdoctoral researcher and collaboration in human reseorces formation in the TOXICOLOGY AND CHEMICAL SAFETY UNIT AT THE INSTITUTE OF BIOENGINEERING, UNIVERSITY MIGUEL HERNANDEZ, ELCHE, ALICANTE. Institution: Universidad Miguel Hernández de Elche Institute: Instituto de Bioingeniería Department: Applied Biology Address: Unidad de Toxicología y Seguridad Química. Instituto de Bioingeniería. Universidad Miguel Hernández de Elche. Avda de la Universidad S/n. 03202 Elche Advisors: Professor. Eugenio Vilanova

2009 - 2013

MIGUEL HERNÁNDEZ UNIVERSITY

Vínculo: Bolsista, Enquadramento Funcional: ph D fellowship, collaboration agreement INIA, Carga horária: 40

Outras informações:
PhD in the TOXICOLOGY AND CHEMICAL SAFETY UNIT AT THE INSTITUTE OF BIOENGINEERING, UNIVERSITY MIGUEL HERNANDEZ, ELCHE, ALICANTE. Institution: Universidad Miguel Hernández de Elche Institute: Instituto de Bioingeniería Department: Applied Biology Address: Unidad de Toxicología y Seguridad Química. Instituto de Bioingeniería. Universidad Miguel Hernández de Elche. Avda de la Universidad S/n. 03202 Elche Advisors: Professor. Eugenio Vilanova and Ph D. Jorge Estévez Research line My research was devoted in mechanistic aspects of organophosphate pesticide toxicology. The focus of my ph D research was to study the molecular interaction of nervous system enzymes (esterases) interacting with organophosphorus compounds. I have been working in the kinetic characterization of the binding of these esterases in order to identify target proteins of organophosphorus toxicity and/or detoxification. This kinetic characterization was used to have criteria for monitoring during isolation and purification studies using separation methods, mainly by HPLC. Tandem mass spectrometry (LC-MS-MS) in the AB SCIEX TripleTOF 5600 System followed by bioinformatic approaches were used to identify molecularly some of these proteins. Peptide mass fingerprinting by mass spectrometry was used to resolve the structures of the inhibited enzyme and of the post-inhibitory modification pathways in purify enzymes. In short, the work implicated was: To programme the laboratory Automation workstation Biomek 2000 (Beckman Instruments) to development an automatable microassay method of enzymatic measurements. Kinetic enzymatic experiments and mathematical analysis of the kinetic data. Protein Extraction methods of cytosolic and membrane `proteins of nervous system tissues. Use Protein Separation and Purification methods: Ultracentrifugation, OFFGEL Electrophoresis, HPLC Chromatography (molecular exclusion, ion exchange, reverse phase...) Use Protein analysis and quantification method

2004 - 2005

UNIVERSITY DEGLI STUDY DI SIENA

Vínculo: Bolsista, Enquadramento Funcional: ERASMUS FELLOWSHIP UNIVERSITY OF SIENA-ITALY, Carga horária: 40, Regime: Dedicação exclusiva.

Outras informações:
Fellowship Erasmus in the University Degli Study di Siena (Italy). October 2004-July 2005.