Albane Le Maire

CNRS Researcher in the Centre de Biochimie Structurale in Montpellier, France. Diploma (equivalent Master Degree) of the Physics and Chemistry Higher Educational Institution (ESPCI, Paris, 2003) and Master Degree in Biological Crystallography and NMR at the Universities Paris V, Paris XI, Grenoble and Strasbourg (France, 2003). Higher Biomedical Training at the University Paris XI (France, 2006). PhD in Structural Biology at the Universiy Pierre et Marie Curie, Paris, (France, 2008). Two-years post-doctoral fellow (2008-2010) for the structural and dynamic studies of nuclear receptors regulation at the Centre de Biochimie Structurale. I have various scientific abilities: Protein crystallization and X-ray crystallography and other structural methods (SAXS and heteronuclear NMR), cloning, mutagenesis, protein expression in bacteria and mammal cells, purification and biochemical analysis of proteins, proteomic analysis (SEC-MALLS, GST pull-down, molecular filtration, immunoprecipitation and mass spectrometry, ITC) and biophysical methods (fluorescent anisotropy, CD-Spetroscopy, dynamic light scattering, Thermofluor). Nuclear receptors are master regulators of gene expression in humans and important drug targets. Their biological functions depend on their ability to bind other molecules such as ligands, DNA and a myriad of coregulatory proteins including epigenetic enzymes. My major interest is to reach a detailed understanding of these recognition processes. By combining structural studies with functional assays, we can build up a comprehensive picture of the molecular mechanisms of transcriptional regulation by nuclear receptors and propose novel regulatory compounds with potential therapeutic applications. The main focus of my current work is in the structural basis of nuclear receptor functions and of epigenetic enzymes involved in nuclear receptor mediated transcription.

Informações coletadas do Lattes em 26/01/2016

Acadêmico

Formação acadêmica

Doutorado em Structural Biology

2003 - 2008

Université Pierre et Marie Curie
Título: Structural and functional study of the human protein KIN17, a protein involved in response to radiation damage
Orientador: Dr. B. Gilquin
Coorientador: Dr. S. Zinn-Justin. Bolsista do(a): Commissariat à l'Energie Atomique.

Mestrado em Biological Crystallography and NMR

2002 - 2003

Université Paris Descartes
Título: NMR structure of the SH3 domain of the human nephrocystin,Ano de Obtenção: 2003
Orientador: Pr. F. Dardel
Coorientador: Pr. A. Ducruix.

Mestrado em Physic-Chemitry

1999 - 2003

Ecole Superieure de Physique et de Chimie Industrielles de La Vl de Paris
Título: Study of the metabolic effects of refeeding after restriction using mice,Ano de Obtenção: 2003
Orientador: Dr. O. Boss

Graduação em Physic-Chemistry-Biology

1997 - 1999

Lycée Sainte Geneviève
Título: Preparation for the competitive entrance exams to Higher Educational Institutions BCPST

Pós-doutorado

2008 - 2010

Pós-Doutorado. , Centre National de la Recherche Scientifique, CNRS, França. , Bolsista do(a): Agence Nationale pour la Recherche.

Formação complementar

2003 - 2006

Higher Biomedical Training. , Université Paris-Sud 11, PARIS-SUD 11, França.

Idiomas

Inglês

Compreende Razoavelmente, Fala Razoavelmente, Lê Bem, Escreve Bem.

Português

Compreende Razoavelmente, Fala Pouco, Lê Pouco, Escreve Pouco.

Francês

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Russo

Compreende Pouco, Fala Pouco, Lê Pouco, Escreve Pouco.

Áreas de atuação

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: Química de Macromoléculas.

Grande área: Ciências Biológicas / Área: Biofísica / Subárea: Biofísica Molecular.

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: Biologia Molecular.

Participação em eventos

EMBO meeting on Nuclear Receptors. A unique secondary-structure switch controls constitutive gene repression by retinoic acid receptor. 2011. (Congresso).

Days of nuclear receptors.Structural biology of nuclear receptors. 2011. (Simpósio).

Keystone meeting on Nuclear Receptors. A unique secondary-structure switch controls constitutive gene silencing by retinoic acid receptor. 2010. (Congresso).

EMBO Conference: Nuclear receptor. Modulation of nuclear receptor activity by organotin environmental endocrine disruptors. 2009. (Congresso).

Dynamic Organization of Nuclear Function meeting. The human KIN17 protein: DNA binding and histone recognition. 2006. (Congresso).

23rd European Crystallography Meeting. Tungstate complexes of the C-terminal domain of human KIN17, a stress activated protein, reveal its RNA binding site. 2006. (Congresso).

Jacques Monod Conference: Regulation of pre-mRNA splicing and human disease. Does the human KIN17 protein participate in RNA metabolism?. 2005. (Congresso).

GTBIo. The use of X-ray crystallography to study the human KIN17 protein. 2004. (Congresso).

Orientou

Barbon Marta

Structural charaterization of the SET domain of MLL5, a human histone methyltransferase; Início: 2014; Dissertação (Mestrado em Master 2 Biological Sciences) - Università degli Studi di Ferrara, ERASMUS; (Orientador);

Turek Simon

Structural studies of the interaction of RAR with corepressors; ; Início: 2014; Dissertação (Mestrado em Master 2) - Universidade de Ljubljana, ERASMUS; (Orientador);

Nadendla Eswar

Structural studies of retinoic acid receptors from different species; Início: 2014; Tese (Doutorado em Biophysic) - VIT University - Chennai Campus, ERASMUS MUNDUS; (Coorientador);

Mas-y-Mas Sarah

Determination of the structure and of the mechanism of action of the human methyltransferase MLL5; 2012; Dissertação (Mestrado em Master 2 BIOMED) - Université Montpellier 2 - Sciences et Techniques,; Orientador: Albane le Maire;

Produções bibliográficas

VAISSIÈRE, ANAÏS ; BERGER, SYLVIE ; HARRUS, DEBORAH ; DACQUET, CATHERINE ; LE MAIRE, ALBANE ; BOUTIN, JEAN A. ; FERRY, GILLES ; ROYER, CATHERINE A. . Molecular mechanisms of transcriptional control by Rev-erbα: An energetic foundation for reconciling structure and binding with biological function. Protein Science (Print) , v. 24, p. 1129-1146, 2015.
DELFOSSE, VANESSA ; MAIRE, ALBANE LE ; BALAGUER, PATRICK ; BOURGUET, WILLIAM . A structural perspective on nuclear receptors as targets of environmental compounds. Acta Pharmacologica Sinica , v. 36, p. 88-101, 2014.
DELFOSSE, V. ; GRIMALDI, M. ; PONS, J.-L. ; BOULAHTOUF, A. ; le Maire, A. ; CAVAILLES, V. ; LABESSE, G. ; BOURGUET, W. ; BALAGUER, P. . Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes. Proceedings of the National Academy of Sciences of the United States of America , v. 109, p. 14930-14935, 2012.
LE MAIRE, ALBANE ; ALVAREZ, SUSANA ; SHANKARANARAYANAN, PATTABHIRAMAN ; R DE LERA, ANGEL ; BOURGUET, WILLIAM ; GRONEMEYER, HINRICH . Retinoid Receptors and Therapeutic Applications of RAR/RXR Modulators. Current Topics in Medicinal Chemistry (Print) , v. 12, p. 505-527, 2012.
LE MAIRE, ALBANE ; GELIN, MURIEL ; POCHET, SYLVIE ; HOH, FRANÇOIS ; PIROCCHI, MICHEL ; GUICHOU, JEAN-FRANÇOIS ; FERRER, JEAN-LUC ; LABESSE, GILLES . In-plate protein crystallization, ligand soaking and X-ray diffraction. Acta Crystallographica. Section D, Biological Crystallography , v. 67, p. 747-755, 2011.
RIU, A. ; le Maire, A. ; GRIMALDI, M. ; AUDEBERT, M. ; HILLENWECK, A. ; BOURGUET, W. ; BALAGUER, P. ; ZALKO, D. . Characterization of Novel Ligands of ER , Er , and PPAR : The Case of Halogenated Bisphenol A and Their Conjugated Metabolites. Toxicological Sciences (Print) , v. 122, p. 372-382, 2011.
RIU, ANNE ; GRIMALDI, MARINA ; LE MAIRE, ALBANE ; BEY, GILBERT ; PHILLIPS, KEVIN ; BOULAHTOUF, ABDELHAY ; PERDU, ELISABETH ; ZALKO, DANIEL ; BOURGUET, WILLIAM ; BALAGUER, PATRICK . Peroxisome Proliferator-Activated Receptor γ Is a Target for Halogenated Analogs of Bisphenol A. Environmental Health Perspectives , v. 119, p. 1227-1232, 2011.
LE MAIRE, ALBANE ; TEYSSIER, CATHERINE ; ERB, CATHIE ; GRIMALDI, MARINA ; ALVAREZ, SUSANA ; DE LERA, ANGEL R ; BALAGUER, PATRICK ; GRONEMEYER, HINRICH ; ROYER, CATHERINE A ; GERMAIN, PIERRE ; BOURGUET, WILLIAM . A unique secondary-structure switch controls constitutive gene repression by retinoic acid receptor. Nature Structural and Molecular Biology , v. 17, p. 801-807, 2010.
LE MAIRE, ALBANE ; BOURGUET, WILLIAM ; BALAGUER, PATRICK . A structural view of nuclear hormone receptor: endocrine disruptor interactions. Cellular and Molecular Life Sciences (Printed ed.) , v. 67, p. 1219-1237, 2010.
LE MAIRE, ALBANE ; GRIMALDI, MARINA ; ROECKLIN, DOMINIQUE ; DAGNINO, SONIA ; VIVAT-HANNAH, VALÉRIE ; BALAGUER, PATRICK ; BOURGUET, WILLIAM . Activation of RXR-PPAR heterodimers by organotin environmental endocrine disruptors. EMBO Reports (Print) , v. 10, p. 367-373, 2009.
CARLIER, LUDOVIC ; COUPRIE, JOËL ; LE MAIRE, ALBANE ; GUILHAUDIS, LAURE ; MILAZZO-SEGALAS, ISABELLE ; COURÇON, MARIE ; MOUTIEZ, MIREILLE ; GONDRY, MURIEL ; DAVOUST, DANIEL ; GILQUIN, BERNARD ; ZINN-JUSTIN, SOPHIE . Solution structure of the region 51-160 of human KIN17 reveals an atypical winged helix domain. Protein Science (Print) , v. 16, p. 2750-2755, 2007.
LE MAIRE, ALBANE ; SCHILTZ, MARC ; STURA, ENRICO A. ; PINON-LATAILLADE, GHISLAINE ; COUPRIE, JOËL ; MOUTIEZ, MIREILLE ; GONDRY, MURIEL ; ANGULO, JAIME F. ; ZINN-JUSTIN, SOPHIE . A Tandem of SH3-like Domains Participates in RNA Binding in KIN17, a Human Protein Activated in Response to Genotoxics. Journal of Molecular Biology , v. 364, p. 764-776, 2006.
LE MAIRE, ALBANE ; SCHILTZ, MARC ; BRAUD, SANDRINE ; GONDRY, MURIEL ; CHARBONNIER, JEAN-BAPTISTE ; ZINN-JUSTIN, SOPHIE ; STURA, ENRICO . Crystallization and halide phasing of the C-terminal domain of human KIN17. Acta Crystallographica. Section F , v. 62, p. 245-248, 2006.
LE MAIRE, ALBANE ; WEBER, THOMAS ; SAUNIER, SOPHIE ; BROUTIN, ISABELLE ; ANTIGNAC, CORINNE ; DUCRUIX, ARNAUD ; DARDEL, FRÉDÉRIC . Solution NMR structure of the SH3 domain of human nephrocystin and analysis of a mutation-causing juvenile nephronophthisis. Proteins (Print) , v. 59, p. 347-355, 2005.
Delfosse, V ; Grimaldi, M ; Bourguet, W ; le Maire, A. ; Balaguer, P . Nuclear Receptor Profiling of Bisphenol-A and Its Halogenated Analogues. Vitamins & Hormones. 94ed.: , 2014, v. , p. 229-251.
le Maire, A. ; Bourguet, W . Retinoic Acid Receptors: Structural Basis for Coregulator Interaction and Exchange. The Biochemistry of Retinoic Acid Receptors I: Structure, Activation, and Function at the Molecular Level. 70ed.: , 2014, v. , p. 37-54.
le Maire, A. ; Bourguet, W ; Gronemeyer, H ; De Lera, A.R. . Retinoic Acid Receptors and their Modulators: Structural and Functional Insights. Vitamin A and Carotenoids Vitamin A and Carotenoids : Chemistry. 24ed.: , 2012, v. , p. 417-437.

Projetos de pesquisa

2015 - Atual

STRUCTURAL STUDIES OF THE INTERACTION OF NORMAL AND PATHOLOGICAL RAR AND THR WITH COREPRESSORS., Descrição: PROGRAMA CIÊNCIA SEM FRONTEIRAS FELLOWSHIP IN BRAZIL YOUNG TALENT ATTRACTION BJT CNPq . , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Albane le Maire - Coordenador.

Prêmios

2007

Fourth year PhD funding, Association pour la Recherche contre le Cancer.

Histórico profissional

Endereço profissional

Centre National de la Recherche Scientifique, Centre de Biochimie Structurale. , 29, route de Navacelles, Montpellier, Montpellier, - França - Caixa-postal: 34090, Telefone: (33) 467417911

Experiência profissional

2015 - Atual

Centro Nacional de Pesquisa em Energia e Materiais

Vínculo: , Enquadramento Funcional:

2015 - Atual

Centre de Biochimie Structurale

Vínculo: Bolsista, Enquadramento Funcional: Visiting Researcher at the LNBIO

2014 - Atual

Centre de Biochimie Structurale

Vínculo: Servidor Público, Enquadramento Funcional: Reseracher CNRS CR1

2010 - 2014

Centre de Biochimie Structurale

Vínculo: Servidor Público, Enquadramento Funcional: Researcher CNRS CR2

2008 - 2010

Centre de Biochimie Structurale

Vínculo: Servidor Público, Enquadramento Funcional: Post-Doctoral fellow

2003 - 2008

Commissariat à la Énergie Atomique - Saclay

Vínculo: Servidor Público, Enquadramento Funcional: PhD student

2001 - 2001

Novartis Pharma-Basel

Vínculo: Bolsista, Enquadramento Funcional: Research work

2008 - 2009

Université Montpellier 2 - Sciences et Techniques

Vínculo: , Enquadramento Funcional: Tutorial in Crystallography in M2 degree, Carga horária: 20

2007 - 2008

Université Paris-Sud 11

Vínculo: Servidor Público, Enquadramento Funcional: Tutorials in cellular signaling in M2 degree, Carga horária: 10