Renata Prado Palma

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Produções bibliográficas

• BANTI, R. S. ; BASDADJIAN, Y. F. ; CRUZ, M. R. C. ; VIOLA, M. F. ; YABIKU, E. H. ; ZINS, T. ; STAICOV, I. ; PESSOA, H. ; PALMA, R. P. . 50´HQC. , 2011.

• PALMA, R. P. . Ciclo de Palestras Instituto Aprenda.bio. 2010. (Apresentação de Trabalho/Outra).

• PALMA, R. P. . Educação Sexual e Gravidez Indesejada o que pensam alunos e professores de uma escola pública e uma particular sobre o tema.. 2010. (Apresentação de Trabalho/Outra).

• PALMA, R. P. . Diversos temas 2010 (Noticias publicadas periodicamente em blog).

Outras produções

Projetos de pesquisa

• 2012 - Atual

  Evaluation of Cathepsins (K, L and S) expression during 3T3-L1 differentiation, Descrição: Objectives: Obesity is a crescent global disease that can trigger type II diabetes, cardiovascular disease and many others health problems. Obesity is characterized by white adipose tissue (WAT) accumulation. Adipocytes proliferate via pre-adipocytes differentiation, which comprehends a well orchestraded sequence of events. Therefore, adipocyte differentiation is key step in development of obesity. Cathepsins play an important role on WAT differentiation, evidenced by the reduction of WAT in Cathepsin K or L KO mice. The objective of this study is to evaluate the expression of Cathepsins K, L and S (CatK, CatL and CatS) during the course of adipocyte differentiation. Methods: 3T3-L1 cell culture was differentiated according to standard protocol, after 2 days of confluence cells were tretated with differentiation cocktail (isobutilmetylxantine, dexamethazone and rosiglitazone), and differentiation followed for additional 8 days. Cells were harvested at 0, 2, 4, 6, 8 and 10 days time points of differentiation. Total RNA was obtained by Trizol and submitted to RT-real time PCR. Expression of differentiation marker (ap2/FABP4), later regulators (C/EBP and PPAR2) and CatK, CatL and S were evaluated. Results: Cathepsin L is expressed at very high levels in 3T3-L1 cells, followed by a high level expression of CatK. CatS is expressed at very low levels in 3T3-L1 cells, when compared to the other Cathepsins. CatK has a steady expression troughout differentiation except for a 2.5 fold increased expression at Day 2. The CatL was not modulated by differentiation. CatS is very poorly expressed in 3T3-L1, however Day 2 and Day 10 showed 10 fold higher levels when compared to Day 0. Conclusion: Cathepsins are expressed in 3T3-L1 cells similarly to WAT. The peak expression of CatK and CatS on Day 2 suggests a possible role in the early stages of adipocytes differentiation. . , Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Renata Prado Palma - Coordenador / Marcelo Augusto Christoffolete - Integrante.