Elizabeth Bilsland

Graduated in agronomic engineering (ESALQ - USP) in 1995 and achieved the PhD in Cell and Molecular Biology from Göteborg University (Sweden) in 2004. Background combines a) broad understanding of yeast biology, b) complex genetic manipulations, c) heterologous protein expression, d) high-throughput screens and e) biochemical assays for validation of high-throughput hits. Has engineered hundreds of yeast strains and plasmids, and applied these in the development on high-throughput (HTP) screening methods. PhD dealt with the functional characterization of the S.cerevisiae protein kinases Rck1p and Rck2p, placing them as downstream effectors of MAPK pathways (Rck2p was the first validated direct target of the MAPK Hog1p). Uncovered a role for the HOG pathway in oxidative stress resistance, highlighting the evolutionary conservation of this pathway with the human counterpart. In a short postdoctoral period with the group of Dr. Jessica A. Downs (Department of Biochemistry, University of Cambridge), described the role of Bre5p in DNA repair. Constructed yeast strains expressing chimeric G-protein coupled receptor pathways and to identify modulators of human GPCRs. Developed a yeast-based screening assay for HTP drug screens for anti-parasitic agents, by labeling strains expressed either a heterologous drug target from a range of different parasites or their human counterpart with 1 of 4 different fluorescent proteins. With collaborators at the University of Aberystwyth (Wales), performed fully automated HTP screens on 3 distinct enzyme targets from 6 different tropical parasites. Developed a system for the identification of transporters responsible for the uptake of cytotoxic compounds. Using yeast strains with deletions of non-essential plasma membrane transporters, could suggest import routes for about half of the anticancer drugs tested, supporting the hypothesis that drugs enter cells via protein transporters, rather than by simple diffusion. Currently developing novel yeast strains expressing potential drug targets from filarial worms and performing screens aimed at the development of novel treatments against elephantiasis.

Informações coletadas do Lattes em 12/04/2019

Acadêmico

Formação acadêmica

Doutorado em PhD in Cell and Molecular Biology

1998 - 2004

Göteborg University, Sweden
Título: The Saccharomyces cerevisiae MAPKAP kinases Rck1 and RCK2 in stress responses
Orientador: Per Sunnerhagen

Graduação em Engenharia Agronômica

1991 - 1995

Universidade de São Paulo
Bolsista do(a): Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

Pós-doutorado

2013 - 0000

Pós-Doutorado. , University of Cambridge. , Bolsista do(a): Bill and Melinda Gates Foundation.

2011 - 2013

Pós-Doutorado. , University of Cambridge. , Bolsista do(a): União Européia.

2008 - 2011

Pós-Doutorado. , University of Cambridge. , Bolsista do(a): Biotechnology and Biological Sciences Research Council. , Grande área: Ciências Biológicas / Área: Genética / Subárea: Biologia sintética. , Grande Área: Ciências Biológicas / Área: Farmacologia / Subárea: Farmacologia Bioquímica e Molecular. , Grande Área: Outros / Área: Robótica, Mecatrônica e Automação.

2004 - 2007

Pós-Doutorado. , University of Cambridge. , Bolsista do(a): Wenner-Gren Stiftelserna. , Grande área: Ciências Biológicas / Área: Genética / Subárea: Genética Molecular e de Microorganismos. , Grande Área: Ciências Biológicas / Área: Bioquímica / Subárea: Bioquímica dos Microorganismos.

Idiomas

Inglês

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Português

Compreende Bem, Fala Bem, Lê Bem, Escreve Bem.

Italiano

Compreende Bem, Fala Razoavelmente, Lê Razoavelmente, Escreve Pouco.

Alemão

Compreende Pouco, Fala Pouco, Lê Pouco, Escreve Pouco.

Suéco

Compreende Bem, Fala Razoavelmente, Lê Razoavelmente, Escreve Pouco.

Áreas de atuação

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: biologia sintética.

Grande área: Ciências Biológicas / Área: Genética / Subárea: Genética Molecular e de Microorganismos.

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: Biologia Molecular.

Grande área: Ciências Biológicas / Área: Bioquímica.

Grande área: Ciências Biológicas / Área: Bioquímica / Subárea: Bioquímica dos Microorganismos.

Grande área: Ciências Biológicas / Área: Farmacologia / Subárea: Toxicologia.

Produções bibliográficas

2013 BILSLAND, E. ; WILLIAMS, K. ; MOSS, H. J. ; DE CLARE, M. ; PIR, P. ; ROWLAND, J. ; AUBREY, W. ; PATEMAN, R. ; YOUNG, M. ; CARRINGTON, M. ; KING, R. D. ; OLIVER, S. G. . Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds. OPEN BIOL , v. 3, p. 120158-120158, 2013.
2013 KELL, DOUGLAS B. ; DOBSON, PAUL D. ; BILSLAND, ELIZABETH ; OLIVER, STEPHEN G. . The promiscuous binding of pharmaceutical drugs and their transporter-mediated uptake into cells: what we (need to) know and how we can do so. Drug Discovery Today , v. 18, p. 218-239, 2013.
2013 FAVRIN, G. ; BEAN, D. M. ; BILSLAND, E. ; BOYER, H. ; FISCHER, B. E. ; RUSSELL, S. ; CROWTHER, D. C. ; BAYLIS, H. A. ; OLIVER, S. G. ; GIANNAKOU, M. E. . Identification of novel modifiers of Aβ toxicity by transcriptomic analysis in the fruitfly. Scientific Reports , v. 3, p. 1, 2013.
2011 LANTHALER, KARIN ; BILSLAND, ELIZABETH ; DOBSON, PAUL D ; MOSS, HARRY J ; PIR, P'NAR ; KELL, DOUGLAS B ; OLIVER, STEPHEN G . Genome-wide assessment of the carriers involved in the cellular uptake of drugs: a model system in yeast. BMC Biology , v. 9, p. 70, 2011.
2011 BILSLAND, ELIZABETH ; PIR, P'NAR ; GUTTERIDGE, ALEX ; JOHNS, ALEXANDER ; KING, ROSS D. ; OLIVER, STEPHEN G. . Functional Expression of Parasite Drug Targets and Their Human Orthologs in Yeast. PLoS Neglected Tropical Diseases (Online) , v. 5, p. e1320, 2011.
2007 BILSLAND, ELIZABETH ; HULT, MALIN ; BELL, STEPHEN D. ; SUNNERHAGEN, PER ; DOWNS, JESSICA A. . The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage. DNA Repair (Print) , v. 6, p. 1471-1484, 2007.
2007 CVIJOVI', MARIJA ; DALEVI, DANIEL ; BILSLAND, ELIZABETH ; KEMP, GRAHAM JL ; SUNNERHAGEN, PER . Identification of putative regulatory upstream ORFs in the yeast genome using heuristics and evolutionary conservation. BMC Bioinformatics , v. 8, p. 295, 2007.
2005 BILSLAND, E. . Tails of histones in DNA double-strand break repair. Mutagenesis , v. 20, p. 153-163, 2005.
2004 BILSLAND, ELIZABETH ; MOLIN, CLAES ; SWAMINATHAN, SWARNA ; RAMNE, ANNA ; SUNNERHAGEN, PER . Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance. Molecular Microbiology (Print) , v. 53, p. 1743-1756, 2004.
2000 BILSLAND-MARCHESAN, E. ; ARINO, J. ; SAITO, H. ; SUNNERHAGEN, P. ; POSAS, F. . Rck2 Kinase Is a Substrate for the Osmotic Stress-Activated Mitogen-Activated Protein Kinase Hog1. Molecular and Cellular Biology (Print) , v. 20, p. 3887-3895, 2000.
RAMNE, A. ; BILSLAND-MARCHESAN, E. ; ERICKSON, S. . The protein kinases Rck1 and Rck2 inhibit meiosis in budding yeast. MGG. Molecular & General Genetics (Print) , v. 263, p. 253-261, 2000.
BILSLAND, ELIZABETH ; DAHLÉN, MARIA ; SUNNERHAGEN, PER . Genomic disruption of six budding yeast genes gives one drastic example of phenotype strain-dependence. Yeast (Chichester, England. Print) , v. 14, p. 655-664, 1998.
Scheurer, C. ; Chugh, M. ; BILSLAND, ELIZABETH ; Van Schalkwyk, D. ; Wicht, K.J. ; Sharma, A. ; Bashyam, S. ; Singh, S. ; Egan, T.J. ; Sutherland, C. ; Beck, H.-P. ; Wittlin, S. ; OLIVER, S. G. ; Malhotra, P. ; Spangenberg, T. ; Ding, X.C. . Deconvolution of New Antimalarial Compounds Cross-Resistance Signals using Multidrug-Resistant Plasmodium falciparum Strains. Antimicrobial Agents and Chemotherapy (Print) , 2014.
BILSLAND, E. . Yeast-based high-throughput screens identify novel antiparasitic drugs. 2013. (Apresentação de Trabalho/Conferência ou palestra).
BILSLAND, E. . A yeast system to identify carriers involved in cellular uptake of drugs. 2013. (Apresentação de Trabalho/Conferência ou palestra).
BILSLAND, E. . Yeast-based high-throughput screens identify novel antiparasitic drugs. 2012. (Apresentação de Trabalho/Congresso).
BILSLAND, E. . Enabling Robot Scientists to Screen for Antiparasitic Drugs. 2011. (Apresentação de Trabalho/Conferência ou palestra).

Outras produções

BILSLAND, E. . Yeast Leads the Way for New Drugs. 2013. (Programa de rádio ou TV/Entrevista).

BILSLAND, ELIZABETH . La levadura, poderosa arma en la lucha contra la malaria. 2013. (Programa de rádio ou TV/Entrevista).

BILSLAND, ELIZABETH . New tool in the fight against tropical diseases. 2013. (Programa de rádio ou TV/Entrevista).

BILSLAND, ELIZABETH . New Tool in the Fight Against Tropical Diseases. 2013. (Programa de rádio ou TV/Entrevista).

BILSLAND, ELIZABETH . New tool in the fight against tropical diseases. 2013. (Programa de rádio ou TV/Entrevista).