Wagner Luis Reis

Pós-doutorado

Formação complementar

Idiomas

Áreas de atuação

Organização de eventos

Participação em eventos

Participação em bancas

Orientou

Produções bibliográficas

• PALHETA, RAIMUNDO C. ; DA SILVA, MOISÉS T. B. ; GEORGII, ANA DÉBORA N. P. ; SILVA, CAMILA M. S. ; SIQUEIRA, RÔMMULO C. L. ; REIS, WAGNER L. ; RUGINSK, SILVIA G. ; ELIAS, LUCILA L. K. ; ANTUNES-RODRIGUES, JOSÉ ; SANTOS, ARMÊNIO A. . Role of atrial natriuretic peptide and oxytocin in gastric emptying delay induced by right atrial stretch in rats. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY , v. 328, p. R396-R407, 2025.

• LORENZON, FLAVIANO ; GREGORIO, TAMIRES ; NIEBISCH, FERNANDA ; STOLTE, RAFAELA CARLA KACHEL ; PEIXE, CAROLINA DE MORAES SILVEIRA ; REIS, Wagner Luis ; DOS SANTOS, GUSTAVO JORGE ; LIMA, FERNANDA BARBOSA . Gestational administration of vitamin D improves maternal care and prevents anxiety-like behavior in male and female Wistar rats prenatally exposed to dexamethasone. LIFE SCIENCES , v. 326, p. 121799, 2023.

• COGNUCK, S. Q. ; Reis, WL ; SILVA, M. S. ; ZORRO, S. V. ; ALMEIDA-PEREIRA, GISLAINE ; DEBARBA, L. K. ; ELIAS, LUCILA L.K. ; Antunes-Rodrigues, J . Age and sex influence the response in lipid metabolism of dehydrated Wistar rats. Scientific Reports , v. 12, p. 9164, 2022.

• ZAIA, CÁSSIA THAÏS BUSSAMRA VIEIRA ; UCHÔA, ERNANE TORRES ; SANTOS, ANGELO ALEXANDER TORRES DOS ; RIBEIRO, RACHEL CEZAR DE ANDRADE ; BATISTA, ANA CAROLINA SEIDEL ; CRESPIGIO, JEFFERSON ; UTIDA, LAWRENCE ; MOURA, GALIANO BRAZUNA ; BROWNLOW, MILENE LARA ; GARNICA-SIQUEIRA, MARCELA CRISTINA ; REIS, Wagner Luis ; ANTUNES-RODRIGUES, JOSE ; ZAIA, DIMAS AUGUSTO MOROZIN . Vasoactive intestinal peptide promotes hypophagia and metabolic changes: Role of paraventricular hypothalamic nucleus and nitric oxide. BRAIN RESEARCH BULLETIN , v. 189, p. 102-110, 2022.

• COGNUCK, SUSANA QUIRÓS ; ALMEIDA, LUCAS FERREIRA DE ; REIS, WAGNER L. ; SILVA, MÁRCIA S. ; ALMEIDA-PEREIRA, GISLAINE ; ZORRO, SANDRA V. ; MECAWI, ANDRÉ S. ; COIMBRA, TEREZILA MACHADO ; ELIAS, LUCILA L. K. ; ANTUNES-RODRIGUES, JOSÉ . Alterations in Kidney Structures Caused by Age Vary According to Sex and Dehydration Condition. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , v. 23, p. 15672, 2022.

• DE MELO, IGOR SANTANA ; SABINO-SILVA, ROBINSON ; CUNHA, THÚLIO MARQUEZ ; GOULART, LUIZ RICARDO ; REIS, Wagner Luis ; JARDIM, ANA CAROLINA GOMES ; SHETTY, ASHOK K. ; DE CASTRO, OLAGIDE WAGNER . Hydroelectrolytic Disorder in COVID-19 patients: Evidence Supporting the Involvement of Subfornical Organ and Paraventricular Nucleus of the Hypothalamus. NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS , v. 124, p. 216-223, 2021.

• TERRA DOS SANTOS, ANA LUIZA ROMERO ; REIS, Wagner Luis ; QUIRÓS'COGNUCK, SUSANA ; LIMA, JULIANA BEZERRA ; DOMINGUES, JULIANA TONIETTO ; ARAÚJO, LEONARDO DOMINGUES ; GREENWOOD, MICHAEL PAUL ; GREENWOOD, MINGKWAN ; ELIAS, LUCILA LEICO KAGOHARA ; MURPHY, DAVID ; ANTUNES'RODRIGUES, JOSÉ . Effects of gonadotropin inducible ovarian transcription factor 1 in the paraventricular nucleus on fluid intake after dehydration of ovariectomized female rats. EXPERIMENTAL PHYSIOLOGY , v. 106, p. 2391-2399, 2021.

• COGNUCK, S. Q. ; REIS, W.L. ; SILVA, M. S. ; ALMEIDA-PEREIRA, G. ; DEBARBA, L. K. ; ZORRO, S. V. ; MECAWI, A. D. S. ; FRANCI, C. R. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . Sex- and age-dependent differences in the hormone and drinking responses to water deprivation.. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY , v. 318, p. R567?R578, 2020.

• BATSCHAUER, TIAGO ; CORDEIRO, JÚLIO M. ; SIMAS, BRUNA B. ; BRUNETTA, HENVER S. ; SOUZA, RAUL M. ; NUNES, EVERSON A. ; REIS, WAGNER L. ; MOREIRA, EDUARDO L.G. ; CRESTANI, CARLOS C. ; SANTOS, ADAIR R.S. ; SPERETTA, GUILHERME F. . Behavioral, cardiovascular and endocrine alterations induced by chronic stress in rats fed a high-fat diet. PHYSIOLOGY & BEHAVIOR , v. 223, p. 113013, 2020.

• COGNUCK, S. Q. ; REIS, W.L. ; SILVA, M. S. ; DEBARBA, L. K. ; MECAWI, A.S. ; PAULA, F. J. A. ; FRANCI, C. R. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Sex differences in body composition, metabolism-related hormones, and energy homeostasis during aging in Wistar rats. PHYSIOLOGICAL REPORTS , v. 8, p. e14597, 2020.

• BIANCARDI, V. C. ; BOMFIM, G. ; Reis, WL ; AL-GASSAMI, S. ; NUNES, K. . The interplay between Angiotensin II, TLR4 and hypertension. PHARMACOLOGICAL RESEARCH , v. 120, p. 88-96, 2017.

• REIS, WAGNER L. ; BIANCARDI, VINICIA C. ; ZHOU, YIQIANG ; STERN, JAVIER E. . A Functional Coupling Between Carbon Monoxide and Nitric Oxide Contributes to Increased Vasopressin Neuronal Activity in Heart Failure rats. Endocrinology (Philadelphia) , v. 157, p. 2052-2066, 2016.

• REIS, W. L. ; BIANCARDI, V. C. ; SON, S. ; ANTUNES-RODRIGUES, J. ; STERN, J. E. . Carbon Monoxide and Nitric Oxide interactions in Magnocellular Neurosecretory Neurones during Water Deprivation. Journal of Neuroendocrinology (Print) , v. 27, p. 111-122, 2015.

• RUGINSK, S. G. ; MECAWI, A. D. S. ; DA SILVA, M. P. ; REIS, W. L. ; COLETTI, R. ; LIMA, J. B. M. D. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . Gaseous Modulators in the Control of the Hypothalamic Neurohypophyseal System. Physiology (Bethesda) , v. 30, p. 127-138, 2015.

• REIS, WAGNER L. ; YI, CHUN-XIA ; GAO, YUANQING ; TSCHÖP, MATHIAS H. ; STERN, JAVIER E. . Brain Innate Immunity Regulates Hypothalamic Arcuate Neuronal Activity and Feeding Behavior. ENDOCRINOLOGY , v. 156, p. 1303-1315, 2015.

• CASSAGLIA, PRISCILA A. ; SHI, ZHIGANG ; LI, BAOXIN ; REIS, WAGNER L. ; CLUTE-REINIG, NICHOLAS M. ; STERN, JAVIER E. ; BROOKS, VIRGINIA L. . Neuropeptide Y acts in the paraventricular nucleus to suppress sympathetic nerve activity and its baroreflex regulation. Journal of Physiology (London. Print) , v. 592, p. n/a-n/a, 2014.

• Antunes-Rodrigues, J. ; Ruginsk, S.G. ; MECAWI, A.S. ; MARGATHO, L.O. ; CRUZ, J.C. ; VILHENA-FRANCO, T. ; REIS, W. L. ; VENTURA, RR ; Reis, L.C. ; VIVAS, L.M. ; Elias, L.L.K. . Mapping and signaling of neural pathways involved in the regulation of hydromineral homeostasis. Brazilian Journal of Medical and Biological Research on line , v. 46, p. 327-338, 2013.

• REIS, W. L. ; BIANCARDI, V. C. ; SON, S. ; ANTUNES-RODRIGUES, J. ; STERN, J. E. . Enhanced Expression of Heme Oxygenase-1 and Carbon Monoxide Excitatory Effects in Oxytocin and Vasopressin Neurones During Water Deprivation. Journal of Neuroendocrinology (Print) , v. 24, p. 653-663, 2012.

• RORATO, RODRIGO ; REIS, WAGNER LUIS ; CARVALHO BORGES, BEATRIZ DE ; ANTUNES-RODRIGUES, JOSÉ ; KAGOHARA ELIAS, LUCILA LEICO . Cannabinoid CB1 receptor restrains accentuated activity of hypothalamic corticotropin-releasing factor and brainstem tyrosine hydroxylase neurons in endotoxemia-induced hypophagia in rats. Neuropharmacology , v. 63, p. 154-160, 2012.

• RORATO, RODRIGO ; REIS, WAGNER LUIS ; ANTUNES-RODRIGUES, JOSÉ ; ELIAS, LUCILA LEICO KAGOHARA . Cholecystokinin and hypothalamic corticotrophin-releasing factor participate in endotoxin-induced hypophagia. Experimental Physiology (Print) , v. Apr;96, p. no-no, 2011.

• REIS, W. L. ; SAAD, W. A. ; Camargo, L.A. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . Central nitrergic system regulation of neuroendocrine secretion, fluid intake and blood pressure induced by angiotensin-II. Behavioral and Brain Functions , v. 6, p. 64, 2010.

• REIS, W. L. ; Giusti-Paiva, A. ; VENTURA, R. R. ; Margatho, L.O. ; GOMES, D. A. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . Neuroendocrinology/Endocrinology: Central nitric oxide blocks vasopressin, oxytocin and atrial natriuretic peptide release and antidiuretic and natriuretic responses induced by central angiotensin II in conscious rats. Experimental Physiology (Print) , v. 92, p. 903-911, 2007.

• REIS, W. L. ; LAUAND, F. ; Ruginsk, S.G. ; RODRIGUES, H. L. P. ; CASTRO, M. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . Glucocorticoid modulation of atrial natriuretic peptide, oxytocin, vasopressin and Fos expression in response to osmotic, angiotensinergic and cholinergic stimulation. Neuroscience , v. 147, p. 247-257, 2007.

• GOMES, D. ; REIS, Wagner Luiz ; VENTURA, R. R. ; Giusti-Paiva, A. ; ELIAS, L. L. K. ; CUNHA, F. Q. ; ANTUNES-RODRIGUES, J. ; Reis, Wagner Luiz . The role of carbon monoxide and nitric oxide in hyperosmolality-induced atrial natriuretic peptide release by hypothalamus in vitro. BRAIN RESEARCH , v. 1016, p. 33-39, 2004.

• VENTURA, R.R. ; GOMES, D.A. ; REIS, W.L. ; Elias, L.L.K. ; CASTRO, M. ; VALENÇA, M.M. ; CARNIO, E.C. ; RETTORI, V. ; MCCANN, S.M. ; ANTUNES-RODRIGUES, J. . Nitrergic modulation of vasopressin, oxytocin and atrial natriuretic peptide secretion in response to sodium intake and hypertonic blood volume expansion. Brazilian Journal of Medical and Biological Research , v. 35, p. 1101-1109, 2002.

• Antunes-Rodrigues, J ; Ruginsk, S.G. ; MECAWI, A. S. ; Margatho, L.O. ; REIS, W. L. ; VENTURA, R. R. ; Silva, A.L. ; VILHENA-FRANCO, T. ; ELIAS, L. L. K. . Chapter 3 - Neuroendocrinology of Hydromineral Homeostasis. In: De Luca LA, Menani JV, Johnson AK. (Org.). Neurobiology of Body Fluid Homeostasis: Transduction and Integration. 1ed.Boca Raton (FL): CRC Press, 2014, v. , p. 1-32.

• REIS, B. P. O. J. G. ; SEVERINO, B. S. ; DEMARCHI, M. ; VIEIRA, B. H. ; REINAUDO, F. D. ; GONCALVES, A. C. ; YABU, G. L. ; LIMA, F. B. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. ; REIS, W. L. . ENVIRONMENTAL ENRICHMENT INCREASES WATER CONSUMPTION AND EXPLORATORY BEHAVIOR IN RATS SUBMITTED TO OSMOTIC STIMULATION. In: II International Congress FloripaNeuro, 2025, Florianópolis, SC - Brasil. II International Congress FloripaNeuro, 2025.

• FERREIRA, J. V. ; REIS, W.L. . PARTICIPAÇÃO NEUROPROTETORA DA PROGRANULINA EM RESPOSTAS COGNITIVAS E COMPORTAMENTAIS DE CAMUNDONGOS SUBMETIDOS A ISQUEMIA VASCULAR CEREBRAL. In: IV Encontro Online do PPGMCF, 2024, Brasil. IV Encontro Online do PPGMCF, 2024.

• REIS, B. P. O. J. G. ; GONCALVES, A. C. ; SEVERINO, B. S. ; YABU, G. L. ; REIS, W. L. . O ENRIQUECIMENTO AMBIENTAL INFLUENCIA A SEDE E O COMPORTAMENTO?. In: IV Encontro Online do PPGMCF, 2024, Brasil. IV Encontro Online do PPGMCF, 2024.

• PERA, M. L. D. ; FILIPINI, R. E. ; LATARO, R. M. ; REIS, W.L. ; VENTURELLI, M. ; SPERETTA, GUILHERME F. . Mobilização passiva dos membros inferiores não alteraa função vascular em pacientes internados por Covid-19. In: XXVII Simpósio Brasileiro de Fisiologia Cardiovascular, 2023, Florianópolis, SC. XXVII Simpósio Brasileiro de Fisiologia Cardiovascular, 2023.

• YABU, G. L. ; ZARDO, B. G. ; FERREIRA, V. P. J. ; GONCALVES, A. C. ; FERREIRA, J. B. ; GREGORIO, T. ; SILVA, L. C. S. ; LATARO, R. M. ; LIMA, F. B. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. ; REIS, W. L. . Central apelin administration does not affect food intake in adult health or obese rats. In: XXXVII Reunião Anual da FeSBE, 2023, Búzios. XXXVII Reunião Anual da FeSBE, 2023.

• FERREIRA, J. V. ; REIS, W. L. . PARTICIPAÇÃO NEUROPROTETORA DA PROGRANULINA E SUAS RESPOSTAS COGNITIVAS E COMPORTAMENTAIS EM CAMUNDONGOS SUBMETIDOS A ISQUEMIA VASCULAR ENCEFALICA. In: III Encontro Online do PPGMCF, 2023, Brasil. III Encontro Online do PPGMCF, 2023.

• FERREIRA, J. B. ; GONCALVES, A. C. ; GREGORIO, T. ; SILVA, M. D. ; ELIAS, LUCILA L.K. ; ANTUNES-RODRIGUES, J. ; LIMA, F. B. ; Reis, WL . Central stimulatory effects of apelin on vasopressin and oxytocin release in adults and elderly female rats. In: XXXVI Reunião Anual da FESBE, 2022. XXXVI Reunião Anual da FESBE, 2022.

• GONCALVES, A. C. ; FERREIRA, J. B. ; GREGORIO, T. ; SILVA, M. D. ; ELIAS, LUCILA L.K. ; ANTUNES-RODRIGUES, JOSÉ ; LIMA, F. B. ; REIS, W. L. . Possible interactions between central apelin and ovarian hormones to control water intake. In: XXXVI Reunião Anual da FESBE, 2022. XXXVI Reunião Anual da FESBE, 2022.

• AMARAL, B. K. ; MARTINS, C. S. ; BACHI, J. ; RIBEIRO, J. A. ; KRAUS, S. I. ; RAMIRES, C. C. ; SILVA, M. D. ; SANTOS, ADAIR R.S. ; PESSOA, A. F. M. ; REIS, Wagner Luis . Nanocarriers using natural compounds accelerates wound healing. In: XXXVI Reunião Anual da FESBE, 2022. XXXVI Reunião Anual da FESBE, 2022.

• FERREIRA, J. V. ; REIS, W. L. . EFEITO NEUROPROTETOR DA PROGRANULINA NO SNC. In: II Encontro Online do Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas - PPGMCF, da Sociedade Brasileira de Fisiologia - SBFis, 2022, Brasil. II Encontro Online do Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas - PPGMCF, da Sociedade Brasileira de Fisiologia - SBFis, 2022.

• PEIXE, C. ; LORENZON, F. ; GREGORIO, T. ; STOLTE, R. ; NIEBISCH, F. ; RAFACHO, A. ; REIS, W. L. ; SANTOS, G. ; LIMA, F. B. . EFFECTS OF GESTATIONAL VITAMIN D ON SHORT/LONG-TERM MEMORY AND COSTICOSTERONE LEVELS AFTER PRENATAL EXPOSURE TO DEXAMETHASONE IN RAT. In: 56º CONGRESSO ANUAL DA SBF, 2021. 56º CONGRESSO ANUAL DA SBF, 2021.

• SANTOS, A. L. T. ; REIS, W.L. ; LIMA, J. ; ARAUJO, L. ; GREENWOOD, M. ; DOMINGUES, J. ; MURPHY, D. ; Elias, L.L.K. ; Antunes-Rodrigues, J . Central Role of Gonadotropin Inducible Ovarian Transcription Factor 1 (Giot1) on Ingestive Behaviour. In: Endocrine Society Meeting ? ENDO 2019, 2019, New Orleans, LA - USA. ENDO 2019, 2019.

• OLIVEIRA, F. S. ; Elias, L.L.K. ; Reis WL ; Antunes-Rodrigues, J. . PAPEL DA APELINA SOBRE O EIXO HIPOTÁLAMO-HIPÓFISE-ADRENAL DE RATOS SUBMETIDOS À PRIVAÇÃO HÍDRICA. In: 26º Simpósio Internacional de Iniciação Científica e Tecnológica da USP - SIICUSP, 2018, Ribeirão Preto. 26º Simpósio Internacional de Iniciação Científica e Tecnológica da USP - SIICUSP, 2018.

• SANTOS, A. L. R. T. ; Elias, L.L.K. ; Reis WL ; Antunes-Rodrigues, J. . PAPEL CENTRAL DO FATOR DE TRANSCRIÇÃO 1 INDUZIDO POR GONADOTROFINAS OVARIANAS EM RESPOSTAS HOMEOSTÁTICAS DO BALANÇO HIDROMINERAL. In: 26º Simpósio Internacional de Iniciação Científica e Tecnológica da USP - SIICUSP, 2018, Ribeirão Preto. 26º Simpósio Internacional de Iniciação Científica e Tecnológica da USP - SIICUSP, 2018.

• Reis, WL ; BORGES, A. G. M. ; CRIVELINI, T. R. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Apelin and carbon monoxide are expressed in vasopressin neurons and mediate water intake during osmotic stimulation. In: 12Th World Congress on Neurohypophysial Hormones, 2017, Mangaratiba, Rio de Janeiro. 12Th World Congress on Neurohypophysial Hormones, 2017.

• Reis, WL ; CRIVELINI, T. R. ; BORGES, A. G. M. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Water intake involves central apelin and carbon monoxide interaction during water deprivation. In: 38Th World Congress of the International Union of Physiological Science, 2017, Rio de Janeiro, RJ. 38Th World Congress of the International Union of Physiological Science, 2017.

• SANTOS, A. L. R. T. ; Reis, WL ; COGNUCK, S. Q. ; GREENWOOD, M. ; GREENWOOD, M. ; MURPHY, D. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Involvement of gonadotropin inducible ovarium transcription factor 1 on oestradiol signal pathways to modulate sodium intake. In: 38Th World Congress of the International Union of Physiological Sciences, 2017, Rio de Janeiro, RJ. 38Th World Congress of the International Union of Physiological Sciences, 2017.

• COGNUCK, S. Q. ; Reis, WL ; SILVA, M. S. ; DEBARBA, L. K. ; COLETTI, R. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Effect of aging and sex on the hormonal responses induced by water deprivtion. In: 38Th World Congress of the International Union of Physiological Sciences, 2017, Rio de Janeiro, RJ. 38Th World Congress of the International Union of Physiological Sciences, 2017.

• REIS, W. L. ; BIANCARDI, V. C. ; ZHOU, Y. ; STERN, J. E. . Carbon monoxide excitatory effect in vasopressin neurons of heart failure rats involves nitric oxide/GABA signaling. In: Experimental Biology (FASEB), 2014, San Diego, CA, USA.. Experimental Biology (FASEB), 2014.

• REIS, W. L. ; BIANCARDI, V. C. ; STERN, J. E. . Anatomical and functional characterization of carbon monoxide (CO) signaling in magnocellular vasopressin (VP) neurons of heart failure (HF) rats. In: World Conference on Neurohypophysial Hormones, 2013, Bristol, UK. World Conference on Neurohypophysial Hormones, 2013.

• REIS, W. L. ; TSCHOEP, M. ; STERN, J. E. . Differential contribution of microglia activation to neuropeptide-Y and pro-opiomelanocortin neurons of the arcuate nucleus. In: The Main Meeting of the Physiology Society (Physiology), 2012, Edinburgh, Scotland UK. The Main Meeting of the Physiology Society (Physiology), 2012.

• REIS, W. L. ; STERN, J. E. . Microglia cells of the arcuate nucleus (ARC) contribute to inhibition of neuropeptide-Y (NPY) neuronal activity induced by toll-like receptor-4 (TLR4) activation. In: Symposium on Recent advances in the study of the Integrative Physiology with emphasis on the neuroendocrine control of energy metabolism and body fluid homeostasis, 2012, Ribeirao Preto. Symposium on Recent advances in the study of the Integrative Physiology with emphasis on the neuroendocrine control of energy metabolism and body fluid homeostasis, 2012.

• REIS, W. L. ; RORATO, R. C. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Nitric oxide modulates vasopressin and oxytocin neuron activity and mRNA expression in hypothalamic paraventricular and supraoptic nuclei induced by extracellular volume expansion. In: World Conference on Neurohypophysial Hormones, 2011, Boston, USA. World Conference on Neurohypophysial Hormones, 2011.

• REIS, W. L. ; BIANCARDI, V. C. ; SON, S. ; STERN, J. E. . Water deprivation increases the expression of heme oxygenase (HO) and carbon monoxide (CO) actions in hypothalamic oxytocin (OT) and vasopressin (VP) neurons. In: World Conference on Neurohypophysial Hormones, 2011, Boston, USA.. World Conference on Neurohypophysial Hormones, 2011.

• REIS, W. L. ; RORATO, R. C. ; Bastianini, L.F.M. ; ELIAS, L. L. K. ; Antunes-Rodrigues, J . Nitric oxide reduces hemorrhage-induced vasopressin and oxytocin neuron activity and mRNA expression in hypothalamic paraventricular and supraoptic nuclei. In: 93th Annual Meeting The Endocrine Society's (ENDO), 2011, Boston, USA. 93th Annual Meeting The Endocrine Society's (ENDO), 2011.

• REIS, W. L. ; RORATO, R. C. ; Bastianini, L.F.M. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . Nitric oxide reduces hypo and hyper volemia-induced vasopressin and oxytocin mRNA expression in hypothalamic paraventricular and supra-optic nuclei.. In: Physiology 2010, 2010, Manchester. The Main Meeting of the Physiology Society, 2010. p. 245-245.

• REIS, W. L. . Óxido nítrico reduz a expressao do RNAm da vasopressina e da ocitocina nos nucleos paraventricular e supraoptico induzida pela hipo e hipervolemia.. In: XXV Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE), 2010, Águas de Lindóia, SP. XXV Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE), 2010.

• REIS, W. L. ; BIANCARDI, V. C. ; ANTUNES-RODRIGUES, J. ; STERN, J. E. . Cellular distribution of heme-oxygenase (HO), a carbon monoxide (CO)-producing enzyme, in the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei. In: Experimental Biology, 2009, New Orleans, Louisiana - USA. The Journal of the Federation of American Societies for Experimental Biology, 2009. v. 23. p. 239-239.

• SILVA, L.E.C.M. ; REIS, W. L. ; CASTRO, M. ; ANTUNES-RODRIGUES, J. ; ELIAS, L. L. K. . Estradiol enhances the sensitivity to central leptin action in ovariectomized rats. In: ENDO 2009, 2009, Washington, DC - USA. ENDO 2009, 2009.

• REIS, W. L. ; Silva, A.L. ; Ruginsk, S.G. ; SILVA, L.E.C.M. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . ?Nitric oxide reduces angiotensin-II-induced vasopressin and oxytocin mRNA expression in hypothalamic paraventricular and supra-optic nuclei.?. In: Physiology 2009, 2009, Dublin - Ireland. The Physiological Society, 2009.

• MEDEIROS, P. ; MEDEIROS, F. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. ; VALENÇA, M. M. . Low doses of propranolol and amitriptylin in migraine prevention: involvement of nitric oxide production. In: Reunião Anual da Federação de Sociedades de Biologia Experimental., 2009, Águas de Lindóia, São Paulo.. FeSBE 2009, 2009.

• TORRES, A.A. ; Sobota, K.N. ; Guarnieri, L.O. ; REIS, W. L. ; Zaia, C.T.B.V. . ?Efeito do peptídeo intestinal vasoativo (VIP) no núcleo paraventricular do hipotálamo (PVN) sobre a produção de óxido nítrico (NO).?. In: Reunião Anual da Federação de Sociedades de Biologia Experimental, 2009, Águas de Lindóia, São Paulo.. FeSBE 2009., 2009.

• REIS, W. L. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . ?Participação do óxido nítrico na regulação neuroendócrina da homeostase hidroeletrolítica.?. In: XXIV Reunião Anual da Federação de Sociedades de Biologia Experimental, 2009, Águas de Lindóia, São Paulo. FeSBE 2009., 2009.

• REIS, W. L. ; BIANCARDI, V. C. ; ANTUNES-RODRIGUES, J. ; STERN, J. E. . Cellular distribution of heme-oxygenase (HO) and nitric oxide synthase (NOS) in the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei of the dehydrated rats. In: Latin American Initiative Symposium, 2009, Ribeirão Preto, São Paulo.. Symposium on Modern Methodologies for the Study of Integrative Physiology, 2009.

• MEDEIROS, F. ; MEDEIROS, P. L. ; REIS, W. L. ; Antunes-Rodrigues, J ; VALENÇA, M. M. . Migraine prevention with low doses of propranolol and amitriptylin: correlation with nitric oxide production. In: 14th Congress of the International Headache Society, 2009, Philadelphia, PA. Cephalalgia, 2009. v. 29. p. 36.

• PALHETA JR, R. C. ; PINHEIRO, A. D. N. ; SILVA, M. T. B. ; Reis WL ; ELIAS, L. L. K. ; Antunes-Rodrigues, J ; SANTOS, A. A. . O peptídeo natriurético atrial participa na retenção gástrica de líquido advindo da distensão atrial em ratos acordados?. In: Reunião Regional da Federação de Sociedades de Biologia Experimental, 2009, Goiânia, GO. FeSBE Regional 2009, 2009.

• REIS, W. L. ; ELIAS, L. L. K. ; SAAD, W. A. ; ANTUNES-RODRIGUES, J. . ?Central nitric oxide reduces vasopressin, oxytocin release and water, saline intake induced by central angiotensin-stimulation?. In: IV Simposium Covian, 2008, Ribeirão Preto, SP, Brasil. IV Simposium Covian, 2008.

• REIS, W. L. ; ELIAS, L. L. K. ; SAAD, W. A. ; ANTUNES-RODRIGUES, J. . ?Central nitric oxide reduce vasopressin, oxytocin release and arterial blood pressure induced by central angiotensin-II stimulation?. In: Physiology 2008, 2008, Cambridge, UK. Physiology 2008, 2008.

• REIS, W. L. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . ?Central L-NAME increases vasopressin, oxytocin and atrial natriuretic peptide release induced by blood volume expansion?. In: ENDO 2008, 2008, San Francisco, California, USA. ENDO 2008, 2008.

• Badauê-Passos, D.Jr. ; de MAGALHAES-NUNES, A. P. ; VENTURA, R. R. ; REIS, W. L. ; REIS, L. C. ; Antunes-Rodrigues, J . Acute administration of 5HT1a serotonin receptor agonist, in the dorsal raphe nuclei (DRN) os systemically, increases whereas peripheral chronic treatment inhibits sodium intake in rats. In: Experimental Biology, 2008, San Diego. The Journal of the Federation of American Societies for Experimental Biology, 2008. v. 22.

• SILVA, G. S. F. ; GIUSTI, H. ; REIS, W. L. ; DIAS, M. B. ; GARGAGLIONI, L. H. ; BRANCO, L. G. S. ; GLASS, M. L. . Involvement of the Nucleus Raphe Obscurus (ROb) on CO2 ventilatory response. In: Experimental Biology, 2008, San Diego. The Journal of the Federation of American Societies for Experimental Biology, 2008. v. 22.

• REIS, W. L. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . "L-NAME intracerebroventricular aumenta a concentração plasmática de vasopressina e ocitocina induzida pela expansão de volume extracelular hipertônica". In: XXII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2007, Águas de Lindóia - Brasil. FeSBE 2007, 2007. p. 110.

• RIBEIRO, R.C.A. ; TORRES, A.A. ; REIS, W. L. . "Peptídeo intestinal vasoativo no núcleo arqueado e paraventricular do hipotálamo e óxido nítrico". In: XXII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2007, Águas de Lindóia - Brasil. FeSBE 2007, 2007. p. 110.

• REIS, W. L. ; Giusti-Paiva, A. ; Margatho, L.O. ; Badauê-Passos, D.Jr. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . "Central nitric oxide blocks vasopressin, oxytocin and atrial natriuretic peptide release induced by central prostaglandin-E2 stimulation". In: 89th Annual Meeting The Endocrine Society's, 2007, Toronto. ENDO 2007, 2007.

• FERREIRA, D. F. ; LOPES, L. M. ; BATISTA, M. B. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. ; SONCINI, R. ; Giusti-Paiva, A. . "Ácido ascórbico reduz a queda da pressão arterial e produção de óxido nítrico durante o choque endotóxico". In: XXII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2007, Águas de Lindóia - Brasil. XXII FeSBE 2007, 2007. p. 57-57.

• ANTUNES-RODRIGUES, J. ; DODICK, D. ; MEDEIROS, F. ; MEDEIROS, P. ; REIS, W. L. ; VALENÇA, M. M. . Simvastatin for migraine prevention and nitric oxide production. In: XIII Congress of the International Headache Society, 2007, Stockholm - Sweden. Cephalalgia, 2007. v. 27. p. 751.

• REIS, W. L. ; Giusti-Paiva, A. ; VENTURA, R. R. ; GOMES, D. A. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . "Central nitric oxide blocks vasopressin and oxytocin release induced by angiotensin-II". In: 88th Annual Meeting of the Endocrine Society, 2006, Boston - Estados Unidos. ENDO 2006, 2006. p. 399.

• REIS, W. L. ; Giusti-Paiva, A. ; Badauê-Passos, D.Jr. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . "Central nitric oxide blocks vasopressin and oxytocin release induced by central hypertonic saline". In: 41st Meeting of the Brazilian Physiological Society & Joint Meeting with The Physiological Society, 2006, Ribeirão Preto - SP. SBFis - Physiology Society, 2006. p. 256.

• REIS, W. L. ; Giusti-Paiva, A. ; Margatho, L.O. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . "Nitric oxide centrally reduce vasopressin and oxytocin release induced by central cholinergic stimulation". In: XXII Congreso Latinoamericano y 1ro Iberoamericano de Ciencias Fisiológicas, 2006, Buenos Aires - Argentina. XXII ALACF 2006, 2006. p. 166-167.

• REIS, W. L. ; Giusti-Paiva, A. ; VENTURA, R. R. ; ANTUNES-RODRIGUES, J. . "Óxido nítrico (NO) centralmente bloqueia a resposta natriurética e andidiurética induzida pela estimulação central com angiotensina-II (ANGII)". In: XX Reunião Anual da Federação de Sociedades de Biologia Experimental, 2005, Águas de Lindóia - SP. XX FeSBE 2005, 2005. p. 141.

• LAUAND, F. ; RODRIGUES, H. L. P. ; REIS, W. L. ; Ruginsk, S.G. ; CASTRO, M. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . ?Pituitary-adrenal axis modulation of natriuretic peptide, oxytocin, vasopressin and c-Fos expression responses to osmotic, angiotensinergic and cholinergic stimulation?. In: Neuroendocrine Network and Body Homeostasis, 2005, Ribeirão Preto - SP. International Symposium of Neuroendocrinology, 2005. p. 56.

• REIS, W. L. ; Giusti-Paiva, A. ; GOMES, D. A. ; ANTUNES-RODRIGUES, J. . "L-NAME intracerebroventricular aumenta a resposta natriurética induzida pela estimulação central com salina hipertônica". In: XIX Reunião Anual da Federação de Sociedades de Biologia Experimental, 2004, Águas de Lindóia - SP. XIX FeSBE 2004, 2004. p. 70.

• GOMES, D. A. ; VENTURA, R. R. ; Giusti-Paiva, A. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. . "Modulação nitrérgica na secreção de ocitocina (OT) em resposta a variação da osmolalidade do meio de incubação". In: XVIII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2003, Curitiba - PR. XVIII FeSBE 2003, 2003. p. 114.

• REIS, W. L. ; Giusti-Paiva, A. ; GOMES, D. A. ; Badauê-Passos, D.Jr. ; VENTURA, R. R. ; Margatho, L.O. ; Silva, M. V. A. ; Holanda, M. ; ANTUNES-RODRIGUES, J. . "Efeito do bloqueio muscarínico sobre a pressão arterial média e a liberação plasmática de ocitocina, vasopressina e peptídeo atrial natriurético induzida pela expansão de volume extracelular hipertônica". In: XXI Congresso da Associação Latino-Americana de Ciências Fisiológicas, 2003, Ribeirão Preto - SP. XXI ALACF 2003, 2003. p. 181-182.

• REIS, W. L. ; Badauê-Passos, D.Jr. ; Giusti-Paiva, A. ; ANTUNES-RODRIGUES, J. . "Efeito da Inibição da enzima conversora de angiotensina (ECA) sobre as alterações hemodinâmicas e excretórias de Na, K e nitrato induzidas pela ocitocina (OT)". In: XVIII Reunião Anual da Federações de sociedades de Biologia Experimental, 2003, Curitiba - PR. XVIII FeSBE 2003, 2003. p. 58.

• GOMES, D. A. ; VENTURA, R. R. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. . "The role of the nitrergic system in the atrial natriuretc peptide release induced by hyperosmolality from hypothalamus in vitro". In: XXI Congresso da Associação Latino-Americana de Ciências Fisiológicas, 2003, Ribeirão Preto - SP. XXI ALACF 2003, 2003. p. 168.

• Giusti-Paiva, A. ; REIS, W. L. ; GOMES, D. A. ; VENTURA, R. R. ; Badauê-Passos, D.Jr. ; Margatho, L.O. ; Silva, M. V. A. ; Holanda, M. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. . "The role of prostaglandins in the LPS-induced vasopressin, oxitocin and prolactin release in rats". In: XXI Congresso da Associação Latino Americana de Ciências Fisiológicas, 2003, Ribeirão Preto - SP. XXI ALACF 2003, 2003. p. 181.

• GOMES, D. A. ; VENTURA, R. R. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. . "Modulação nitrérgica na secreção de ocitocina (OT) pelo hipotálamo in vitro em resposta a estimulação osmótica hipertônica". In: III Simpósio Miguel Rolando Covian, 2003, Ribeirão Preto - SP. III Simpósio Miguel Rolando Covian, 2003.

• VENTURA, R. R. ; GOMES, D. A. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. . "Modulação vasopressinérgica da secreção de ocitocina (OT) e do peptídeo natriurético atrial (ANP) em resposta ao aumento de osmolaridade: participação do óxido nítrico (NO)". In: III Simpósio Miguel Rolando Covian, 2003, Ribeirão Preto - SP. III Simpósio Miguel Rolando Covian, 2003.

• REIS, W. L. ; Badauê-Passos, D.Jr. ; PICANÇO-DINIZ, D. L. W. ; ANTUNES-RODRIGUES, J. . "Redução por captopril da excreção renal de eletrólitos e nitrato estimulada por ocitocina". In: X Simpósio Internacional de Iniciação Científica da Universidade de São Paulo, 2002, Ribeirão Preto - SP. X SIICUSP 2002, 2002.

• GOMES, D. A. ; VENTURA, R. R. ; VALENÇA, M. M. ; REIS, W. L. ; ANTUNES-RODRIGUES, J. . "Efeito do peptídeo natriurético atrial (ANP) sobre a liberação de ocitocina (OT) e vasopressina (AVP) pelo hipotálamo médio basal in vitro". In: XVII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2002, Salvador - BA. XVII FeSBE 2002, 2002. p. 86.

• VENTURA, R. R. ; REIS, W. L. ; GOMES, D. A. ; VALENÇA, M. M. ; PICANÇO-DINIZ, D. L. W. ; ANTUNES-RODRIGUES, J. . "Efeito do antagonista de receptor V1 de vasopressina (AVP) na produção de nitrato estimulada por expansão hipertônica do líquido extracelular". In: XVII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2002, Salvador - BA. XVII FeSBE 2002, 2002. p. 110.

• VENTURA, R. R. ; GOMES, D. A. ; REIS, W. L. ; ELIAS, L. L. K. ; CASTRO, M. ; VALENÇA, M. M. ; CÁRNIO, E. C. ; RETTORI, V. ; MCCANN, S. M. ; ANTUNES-RODRIGUES, J. . "Modulação nitrérgica da secreção de vasopressina, ocitocina e do peptídeo natriurético atrial em resposta a expansão hipertônica do líquido extracelular". In: XVII Reunião Anual da Federação de Sociedades de Biologia Experimental, 2002, Salvador - BA. XVII FeSBE 2002, 2002.

• REIS, W.L. . Técnica de Imunofluorescência. 2024. (Apresentação de Trabalho/Conferência ou palestra).

• REIS, W.L. . Regulação do equilíbrio Hidroeletrolítico. 2022. (Apresentação de Trabalho/Conferência ou palestra).

• Reis WL . Regulação Neuroendócrina e Comportamental dos Líquidos Corporais. 2019. (Apresentação de Trabalho/Conferência ou palestra).

• REIS, W. L. . Gases moduladores em neurônios hipotalâmicos: participação central da apelina. 2019. (Apresentação de Trabalho/Seminário).

• Wagner Luis Reis . Participação dos neurotransmissores gasosos NO e CO na ativação neuroendócrina de ratos com insuficiência cardíaca. 2019. (Apresentação de Trabalho/Seminário).

• REIS, W.L. . Carbon monoxide contributes to increased vasopressin neuronal activity in heart failure rats. 2019. (Apresentação de Trabalho/Conferência ou palestra).

• REIS, W.L. . Involvement of NO and CO on drinking behavior and neuroendocrine hormones releases. 2019. (Apresentação de Trabalho/Simpósio).

• REIS, W. L. . Hypothalamic apelin, putative role in body fluid homeostasis during aging. 2019. (Apresentação de Trabalho/Simpósio).

• Reis WL . New mediators in hydroelectrolyte balance. 2018. (Apresentação de Trabalho/Conferência ou palestra).

• Reis WL . Exercício Físico em Condições de Estresse e Portares de Doenças Crônicas. 2018. (Apresentação de Trabalho/Conferência ou palestra).

• Reis WL . Efeitos centrais da apelina na osmorregulação de animais submetidos a privação hídrica: envolvimento do neurotransmissores gasosos NO e CO. 2018. (Apresentação de Trabalho/Seminário).

• REIS, W. L. . Diferentes contribuições da micróglia induzida pela ativação do TLR4 em neurônios NPY e POMC do Núcleo Arqueado. 2018. (Apresentação de Trabalho/Simpósio).

• REIS, W. L. . Central apelin effects on drinking behavior and neuroendocrine hormones release: involvement of CO and NO functions. 2017. (Apresentação de Trabalho/Simpósio).

• Reis, WL . Gases as modulators of magnocellular neurosecretory neurons. 2016. (Apresentação de Trabalho/Simpósio).

• REIS, W. L. ; STERN, JAVIER E. . Differential contribution of microglia to NPY and POMC neurons of the arcuate nucleus induced by TLR4 activation. 2015. (Apresentação de Trabalho/Simpósio).

• REIS, W. L. ; STERN, JAVIER E. . Carbon Monoxide and Nitric Oxide Interactions in Magnocellular Neurosecretory Neurons. 2015. (Apresentação de Trabalho/Congresso).

• Reis, WL . Enhanced expression of Heme Oxygenese-1 and Carbon Monoxide excitatory effects in Oxytocin and Vasopressin Neurons during Water Deprivation. 2012. (Apresentação de Trabalho/Conferência ou palestra).

• REIS, W. L. ; Antunes-Rodrigues, J . Volemic changes induce neuronal NOS overexpression which modulates AVP and OT secretion. 2010. (Apresentação de Trabalho/Simpósio).

• REIS, W. L. ; BIANCARDI, V. C. ; ELIAS, L. L. K. ; ANTUNES-RODRIGUES, J. ; STERN, J. E. . Osmotic-driven NO/CO interactions in identified magnocellular AVP and OT neurons of the hypothalamic SON and PVN nuclei. 2009. (Apresentação de Trabalho/Simpósio).

Outras produções

Projetos de pesquisa

• 2025 - Atual

  Papel da ocitocina nos efeitos do enriquecimento ambiental na sede e nos comportamentos cognitivos e emocionais em ratas submetidas a privação hídrica, Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (3) / Mestrado acadêmico: (2) . , Integrantes: Wagner Luis Reis - Coordenador / Bruna Pedroso Oliveira Jungles Gonçalves dos Reis - Integrante.

• 2023 - Atual

  Efeitos do Enriquecimento Ambiental na Sede e nos Comportamentos Cognitivos e Emocionais em Ratos Submetidos ao estímulo osmótico - Influência da Angiotensina II, Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Coordenador / Pedroso Oliveira Jungles Gonçalves dos Reis - Integrante.

• 2021 - 2022

  A utilização de nanocarreadores na aceleração do processo de cicatrização de lesões cutâneas induzidas em camundongos., Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Coordenador / Bruna Karla Amaral - Integrante.

• 2021 - Atual

  Participação neuroprotetora da progranulina nas respostas cognitivas e comportamentais de camundongos submetidos a isquemia vascular cerebral., Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Coordenador / Jonata Vital Ferreira - Integrante.

• 2019 - Atual

  Homeostase hidroeletrolítica e energética: do metabolismo celular aos sistemas endócrinos em diferentes fases do desenvolvimento, Situação: Em andamento; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / José Antunes Rodrigues - Integrante / Lucila Leico Kagohara Elias - Coordenador / Francisco José A. de Paula - Integrante / Jorge Elias Júnior - Integrante / Lauro Wichert-Ana - Integrante / Carlos E. Garrido Salmon - Integrante / Livia Mermejo - Integrante.

• 2018 - Atual

  Participação hipotalâmica da apelina na ingestão alimentar durante a obesidade, Descrição: Avaliar a participação da apelina hipotalâmica no controle da ingestão alimentar Investigar se as principais ações centrais da apelina são dependentes dos gases NO e CO.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (2) / Mestrado acadêmico: (1) . , Integrantes: Wagner Luis Reis - Coordenador.

• 2018 - Atual

  Papel central da apelina na atividade neuronal hipotalâmica e comportamento hídrico durante o envelhecimento, Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (2) . , Integrantes: Wagner Luis Reis - Coordenador.

• 2015 - 2018

  Interação anatômica e funcional entre apelina e neurotransmissores gasosos na regulação dos neurônios magnocelulares hipotalâmicos durante alteração da osmolalidade plasmática, Descrição: Dados da literatura apontam que a apelina, identificada recentemente como um endógeno ligante de receptores órfãos acoplados à proteína-G, participa no controle hidroeletrolítico. De fato, o principal papel fisiológico da apelina nestas condições de alteração da osmolalidade plasmática, parece ser oposta ao da vasopressina. Mais do que isso, foi demonstrado que privação hídrica aumenta significativamente a expressão de apelina, HO1 e NOS nos núcleos paraventricular e supra-óptico do hipotálamo. Porém, a aparente interação entre apelina e os gases neurotransmissores CO e NO no controle osmótico permanece desconhecida. Dados preliminares deste projeto revelaram que a apelina inibe atividade neural da VP, via ativação de NO. Assim, para esclarecer este mecanismo, será avaliado: a) a distribuição e expressão dos sistemas apelinérgico, nitrérgico e do CO em astrócitos e em neurônios VP e OT do hipotálamo durante estimulação osmótica; b) a participação do NO e CO nos mecanismos funcionais da apelina na regulação de VP e OT, bem como na ingestão de água, frente a estimulação osmótica. Portanto, usando técnicas de eletrofisiologia, radioimunoensaio, imunofluorescência, PCR em tempo real e de avaliação comportamental, testaremos a hipótese de que os gases NO e CO medeiam os efeitos inibitórios da apelina no controle da homeostase hidroeletrolítica.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (3) . , Integrantes: Wagner Luis Reis - Integrante / ANTUNES-RODRIGUES, JOSÉ - Coordenador., Financiador(es): Conselho Nacional de Desenvolvimento Científico e Tecnológico - Auxílio financeiro.

• 2012 - 2014

  Cross talking between nitric oxide and carbon monoxide in the neuroendocrine regulation during heart failure, Descrição: Neurohumoral activation, which includes elevated circulating levels of the neurohormone vasopressin (VP), is a hallmark in patients with heart failure (HF), being directly correlated to morbidity and mortality in this disease. Despite the pathophysiological importance of neurohumoral activation, the precise underlying mechanisms contributing to elevated VP activation in HF remains unknown. Carbon monoxide (CO) is gaseous neurotransmitter in the central nervous system that stimulates VP neuronal firing activity. Recently, we showed that the excitatory effect of CO on VP neurons in the hypothalamic paraventricular nucleus (PVN) was largely mediated by inhibition of nitric oxide (NO), another key gaseous molecule in the PVN. Given that previous studies showed that VP neuronal activity is enhanced, while NO inhibitory signaling is blunted in HF rats, we tested here whether an enhanced endogenous CO availability within the PVN contributes to enhanced VP neuronal activity and blunted NO signaling in HF rats. Using PVN tissue obtained from sham and HF rats, we showed that both haeme-oxygenase 1(HO-1, the CO-synthesizing enzyme) protein and mRNA expression levels were enhanced in HF rats ( ̴18% and ̴38% respectively). Using patch-clamp electrophysiology, we found that in sham rats, bath application of a CO donor (CORM) increased the firing activity of identified PVN VP neurons (P<0.05), while inhibition of endogenous CO production (SnPP) failed to affect neuronal activity. In HF rats however, SnPP decreased VP neuronal activity (P<0.05), an effect that was occluded by previous NOS blockade (LNAME). Finally, we found that SnPP increased the mean frequency of GABA inhibitory postsynaptic currents (IPSCs) in VP neurons from HF (P<0.05) but not from sham rats. Taken together, these results support an enhanced endogenous CO excitatory signaling in VP neurons from HF rats, which likely contributes to blunted NO and GABA inhibitory function during this condition.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / STERN, JAVIER E. - Coordenador., Financiador(es): American Heart Association - Bolsa.

• 2011 - 2013

  The role of microglia activation in arcuate NPY and POMC neurons during feeding behavior, Descrição: Hypothalamic inflammation, involving microglia activation in the arcuate nucleus (ARC), is proposed as a novel underlying mechanism in obesity, insulin and leptin resistance. However, whether activated microglia affects ARC neuronal activity, and consequently basal and hormonal-induced food intake, is unknown. We show that lipopolysaccharide (LPS), an agonist of the toll-like receptor-4 (TLR4), which we found to be expressed in ARC microglia, inhibited the firing activity of the majority of orexigenic agouti gene-related protein (AgRP/NPY) neurons, while it increased the activity of the majority of anorexigenic proopiomelanocortin (POMC) neurons. LPS effects in AgRP/NPY (but not in POMC) neurons were occluded by inhibiting microglia function or by blocking TLR4 receptors. Finally, we report that inhibition of hypothalamic microglia altered basal food intake, preventing also central orexigenic responses to ghrelin. Our studies support a major role for a TLR4-mediated microglia signaling pathway in the control of ARC neuronal activity and feeding behavior.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / STERN, JAVIER E. - Coordenador.

• 2009 - 2012

  The role of hypothalamic corticotrophin-releasing factor in the endotoxin-induced hypophagia, Descrição: It is well known that endocannabinoids play an important role in the regulation of food intake and body weight. Endocannabinoids and cannabinoid receptors are found in the hypothalamus and brainstem, which are central areas involved in the control of food intake and energy expenditure. Activation of these areas is related to hypophagia observed during inflammatory stimulus. This study investigated the effects of cannabinoid (CB1) receptor blockade on lipopolysaccharide (LPS)-induced hypophagia. Male Wistar rats were pretreated with rimonabant (10 mg/kg, by gavage) or vehicle; 30 min later they received an injection of either LPS (100 mg/kg, intraperitoneal) or saline. Food intake, body weight, corticosterone response, CRF and CART mRNA expression, Fos-CRF and Fos-a-MSH immunoreactivity in the hypothalamus and Fos-tyrosine hydroxylase (TH) immunoreactivity in the brainstem were evaluated. LPS administration decreased food intake and body weight gain and increased plasma corticosterone levels and CRF mRNA expression in the PVN. We also observed an increase in Fos-CRF and Fos-TH double-labeled neurons after LPS injection in vehicle-pretreated rats, with no changes in CART mRNA or Fos-a-MSH immunoreactive neurons in the ARC. In saline-treated animals, rimonabant pretreatment decreased food intake and body weight gain but did not modify hormone response or Fos expression in the hypothalamus and brainstem compared with vehicle-pretreated rats. Rimonabant pretreatment potentiated LPS-induced hypophagia, body weight loss and Fos-CRF and Fos-TH expressing neurons. Rimonabant did not modify corticosterone, CRF mRNA or Fos-a-MSH responses in rats treated with LPS. These data suggest that the endocannabinoid system, mediated by CB1 receptors, modulates hypothalamic and brainstem circuitry underlying the hypophagic effect during endotoxemia to prevent an exaggerated food intake decrease.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / Lucila Leiko K Elias - Coordenador / José Antunes Rodrigues - Integrante / Rodrigo Cesar Rorato - Integrante.

• 2008 - 2014

  Hypothalamic NO and CO interactions and its impacts on magnocellular neuroendocrine functions during osmotic stimulation, Descrição: Nitric oxide (NO) and carbon monoxide (CO) are two diffusible gas signaling messengers in the brain. Previously, we have shown their independent involvement in central fluid/electrolyte homeostasis control. Here, we investigated a possible anatomical and functional interaction between NO/CO in the regulation of vasopressin (VP) and oxytocin (OT) magnocellular neurosecretory cells (MNCs) activity in euhydrated (EU) and dehydrated (48h water deprived (48WD)) rats. Using brain slices from EU and 48WD rats, we measured by immunohistochemistry the expression of neuronal NO synthase (nNOS, which synthesizes NO) and heme-oxygenase (HO-1, which synthesizes CO) in the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei. In addition, we used patch-clamp electrophysiology to investigate whether regulation of MNC firing activity by endogenous CO was dependent on NO bioavailability. We found a high degree of HO-1 and nNOS colocalization within individual MNCs, which was significantly increased in 48 WD rats. This increase affected both VP and OT MNCs, although a more predominant effect was observed in the latter. Inhibition of endogenous HO activity (CrMP 20µM) induced membrane hyperpolarization and diminished MNC firing activity, effects that were reduced by blocking endogenous NOS activity (LNAME 2mM). However, no significant changes on SON NO bioavailability (DAF2-DA fluorescence) was observed after CrMP treatment. Taken together, our results support a state-dependent anatomical/functional interrelationship between NO and CO in MNCs, in which CO acts as an excitatory gas molecule, whose effects are largely dependent on interactions with NO.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / José Antunes Rodrigues - Integrante / Javier E. Stern - Coordenador.

• 2006 - 2010

  Central nitrergic system modulates hormonal responses of vasopressin, oxytocin and atrial natriuretic peptide secretion induced by acute extracellular volume changes, Descrição: We have demonstrated that nitric oxide (NO) is involved in the regulation of vasopressin (VP) and oxytocin (OT) secretion. In this study, the induction of the neuronal nitric oxide synthase (nNOS) in hypothalamic nuclei was evaluated after changes in blood volume and plasma osmolality. Moreover, we examined the role of central nitrergic system on VP and OT gene expression and immunoactivation in the paraventricular (PVN) and supraoptic (SON) nuclei of rats submitted to isotonic (I-) or hypertonic (H-) extracellular volume expansion (EVE). Acute I- and H- EVE stimulation induced an increase in nNOS mRNA expression and immunoactivation of PVN and SON. OT plasma levels and OT mRNA gene expression and immunoactivation of PVN and SON were increased by both I- and H- EVE. However, only H- EVE increased plasma VP concentration, and VP gene expression and immunoactivation of PVN and SON. Central pretreatment with L-NAME (NOS inhibitor) promoted a down-regulation of nNOS gene expression. Furthermore, the NOS inhibition enhanced the increase of OT release and hypothalamic immunoactivation in response to I- and H- EVE. Additionally, L-NAME increased VP secretion, mRNA expression and immunoactivation following I- EVE, and potentate VP release and immunoactivation by H- EVE. On the contrary, central administration of SNAP (NO donor) diminished plasma OT levels, and OT expression in the PVN and SON induced by I- EVE, but the exogenous injection of NO reduced H- EVE-induced VP and OT plasma concentration and hypothalamic gene expression. Thus, induction of nNOS was associated with fluid homeostasis unbalance. Besides, the central nitrergic system can act inhibiting the gene expression, immunoactivation and release of VP and OT from hypothalamic nucleus in response to changes in blood volume and plasma osmolality.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / José Antunes Rodrigues - Coordenador., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Auxílio financeiro.

• 2003 - 2006

  Central nitric oxide blocks vasopressin, oxytocin and atrial natriuretic peptide release and antidiuretic and natriuretic responses induced by central angiotensin-II, Descrição: The presence of nitric oxide synthase (NOS), the enzyme that catalyses the formation of nitric oxide (NO), in the circumventricular organs and magnocellular neurones suggests an important role of NO in the modulation of vasopressin (AVP) and oxytocin (OT) release. Intracerebroventricular (I.C.V.) injection of angiotensin II (Ang II) stimulates the release of AVP, OT and atrial natriuretic peptide (ANP), with the resultant antidiuretic and natriuretic effects. This study investigated the interaction between nitrergic and angiotensinergic pathways on the release of AVP, OT and ANP and on urinary volume and sodium excretion in waterloaded rats. Unanaesthetized, freely moving, maleWistar rats received two water loads followed by an injection into the lateral ventricle of an inhibitor of NOS (L-NAME), a NO donor [3-morpholinylsydnoneimine chloride (SIN-1) or S-nitroso-N-acetyl penicillamine (SNAP)] or vehicle (isotonic saline) and, 20 min after, they received a second I.C.V. injection of Ang II or vehicle. Injections of L-NAME or Ang II produced an increase in plasma levels of AVP, OT and ANP, a reduction in urinary volume and an increase in sodium excretion. Pretreatment with L-NAME enhanced the Ang II-induced increase in AVP, OT and ANP release, as well as the antidiuresis and natriuresis. Injection of SIN-1 or SNAP did not modify hormonal plasma levels and urinary parameters. In contrast SNAP blocked the AVP, OT and ANP release, as well as antidiuretic and natriuretic responses induced by ANG-II. Thus, the central nitrergic system can act to inhibit AVP, OT and ANP secretion and the antidiuretic and natriuretic effects in response to Ang II.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Wagner Luis Reis - Integrante / José Antunes Rodrigues - Coordenador., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Auxílio financeiro.

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