Daniel Braga de Lima

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Áreas de atuação

Participação em eventos

Participação em bancas

Produções bibliográficas

• HASAN, MAHMUDUL ; SCHULZE, SABRINA ; BERNDT, LEONA ; PALM, GOTTFRIED J. ; BRAGA, DANIEL ; RICHTER, INGRID ; LAST, DANIEL ; LAMMERS, MICHAEL ; LACKNER, GERALD . Diversification by CofC and Control by CofD Govern Biosynthesis and Evolution of Coenzyme F and Its Derivative 3PG-F. mBio , v. 13, p. e03501-21, 2022.

• LAST, DANIEL ; HASAN, MAHMUDUL ; ROTHENBURGER, LINDA ; BRAGA, DANIEL ; LACKNER, GERALD . High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space. METABOLIC ENGINEERING , v. 73, p. 158-167, 2022.

• UM, SOOHYUN ; GUO, HUIJUAN ; THIENGMAG, SIRINTHRA ; BENNDORF, RENÉ ; MURPHY, ROBERT ; RISCHER, MAJA ; BRAGA, DANIEL ; POULSEN, MICHAEL ; DE BEER, Z. WILHELM ; LACKNER, GERALD ; BEEMELMANNS, CHRISTINE . Comparative Genomic and Metabolic Analysis of sp. RB110 Morphotypes Illuminates Genomic Rearrangements and Formation of a New 46-Membered Antimicrobial Macrolide. ACS Chemical Biology , v. 16, p. 1482-1492, 2021.

• BRAGA, DANIEL ; HASAN, MAHMUDUL ; KROBER, T. ; LAST, DANIEL ; LACKNER, GERALD . Biosynthesis of the redox coenzyme F420 in Thermomicrobia involves reduction by standalone nitroreductase superfamily enzymes. APPLIED AND ENVIRONMENTAL MICROBIOLOGY (ONLINE) , v. 86, p. e00457, 2020.

• PEÑA-ORTIZ, LUIS ; GRAÇA, ANA P ; GUO, HUIJUAN ; BRAGA, DANIEL ; KOELLNER, TOBIAS ; REGESTEIN, LARS ; BEEMELMANNS, CHRISTINE ; LACKNER, GERALD . Structure elucidation of the redox cofactor mycofactocin reveals oligo-glycosylation by MftF. Chemical Science , v. 11, p. 5182-5190, 2020.

• KUFS, JOHANN E. ; HOEFGEN, SANDRA ; RAUTSCHEK, JULIA ; BISSELL, ALEXANDER U. ; GRAF, CAROLA ; FIEDLER, JONAS ; BRAGA, DANIEL ; REGESTEIN, LARS ; ROSENBAUM, MIRIAM A. ; THIELE, JULIAN ; VALIANTE, VITO . Rational design of flavonoid production routes using combinatorial and precursor-directed biosynthesis. ACS Synthetic Biology , v. 9, p. 1823-1832, 2020.

• BRAGA, DANIEL ; LAST, DANIEL ; HASAN, MAHMUDUL ; GUO, HUIJUAN ; LEICHNITZ, DANIEL ; UZUM, ZERRIN ; RICHTER, INGRID ; SCHALK, FELIX ; BEEMELMANNS, CHRISTINE ; HERTWECK, CHRISTIAN ; LACKNER, GERALD . Metabolic pathway rerouting in Paraburkholderia rhizoxinica evolved long-overlooked derivatives of coenzyme F420. ACS Chemical Biology , v. 14, p. 2088-2094, 2019.

• LENZ, CLAUDIUS ; WICK, JONAS ; BRAGA, DANIEL ; GARCIA-ALTARES, MARIA ; LACKNER, GERALD ; HERTWECK, CHRISTIAN ; GRESSLER, MARKUS ; HOFFMEISTER, DIRK . Injury-Triggered Blueing Reactions of Psilocybe ¿Magic¿ Mushrooms. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION , v. 59, p. 1450-1454, 2019.

• BRANDENBURGER, EILEEN ; BRAGA, DANIEL ; KOMBRINK, ANJA ; LACKNER, GERALD ; GRESSLER, JULIA ; KÜNZLER, MARKUS ; HOFFMEISTER, DIRK . Multi-genome analysis identifies functional and phylogenetic diversity of basidiomycete adenylate-forming reductases. FUNGAL GENETICS AND BIOLOGY , v. 112, p. 55-63, 2018.

• Klapper, M. ; BRAGA, DANIEL ; Herbst, R. ; LACKNER, G. ; Stallforth, P. . Bacterial Alkaloid Biosynthesis: Structural Diversity via a Minimalistic Nonribosomal Peptide Synthetase. Cell Chemical Biology , v. 25, p. 1-7, 2018.

• GUO, HUIJUAN ; SCHMIDT, ALEXANDER ; STEPHAN, PHILIPP ; RAGU', LUKA ; DANIEL BRAGA, DANIEL ; KAISER, MARCEL ; DAHSE, HANS-MARTIN ; WEIGEL, CHRISTIANE ; LACKNER, GERALD ; BEEMELMANNS, CHRISTINE . Precursor-directed Diversification of Cyclic Tetrapeptidic Pseudoxylallemycins. CHEMBIOCHEM , v. 19, p. 2307-2311, 2018.

• BRAGA, DANIEL ; LACKNER, GERALD . One Ring to Fight Them All: The Sulfazecin Story. Cell Chemical Biology , v. 24, p. 1-2, 2017.

• BRAGA, DANIEL ; HOFFMEISTER, DIRK ; NETT, MARKUS . A non-canonical peptide synthetase adenylates 3-methyl-2-oxovaleric acid for auriculamide biosynthesis. Beilstein Journal of Organic Chemistry , v. 12, p. 2766-2770, 2016.

• Agustini, Bruna Carla ; Lima, Daniel Braga de ; Bonfim, Tania Maria Bordin . Composition of amino acids and bioactive amines in common wines of Brazil. Acta Scientiarum. Health Sciences (Online) , v. 36, p. 225-233, 2014.

• PINTO, F. C. J. ; LIMA, D. B. ; AGUSTINI, B. C. ; DALLAGASSA, C. B. ; SHIMABUKURO, M. F. ; CHIMELLI, M. ; BRAND, D. ; FADEL-PICHETH, C. M. T. ; BONFIM, T. M. B. . Morphological and molecular identification of filamentous fungi isolated from cosmetic powders. Brazilian Archives of Biology and Technology (Impresso) , v. 55, p. 897-901, 2012.

• Agustini, Bruna Carla ; Lima, Daniel Braga de ; BRAND, DEBORA DEBORA ; BONFIM, TANIA M. B. . Influência de etapas do processo de vinificação na acidez de vinhos elaborados com uvas Vitis labrusca. BBR - Biochemistry and Biotechnology Reports , v. 1, p. 23-27, 2012.

• LIMA, D. B. ; Agustini, Bruna Carla ; Silva, Eliz Guimarães da ; Gaensly, Fernanda ; Cordeiro, Rodrigo Becker ; Fávero, Maria Luiza Drechsel ; Brand, Debora ; Maraschin, Marcelo ; Bonfim, Tania Maria Bordin . Evaluation of phenolic compounds content and in vitro antioxidant activity of red wines produced from Vitis labrusca grapes. Ciência e Tecnologia de Alimentos (Impresso) , v. 31, p. 783-800, 2011.

• BRAGA, DANIEL ; KOMBRINK, ANJA ; KÜNZLER, MARKUS ; AEBI, M. ; HOFFMEISTER, D. . Characterisation of the adenylation domains of Coprinopsis cinerea reductases induced through inter-kingdom interaction. In: Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM), 2016, Jena. Biospektrum, 2016.

• AGUSTINI, B. C. ; LIMA, D. B. ; BRAND, D. ; BONFIM, T. M. B. . Influência de etapas do processo de vinificação na acidez de vinhos elaborados com uvas Vitis labrusca. In: I Simpósio de Bioquímica e Biotecnologia, 2011, Londrina. Caderno de Resumos SIMBBTEC 2011, 2011.

• LIMA, D. B. ; Sassaki, G. L. . Obtenção de derivados isopropilidínicos de manofuranose: octil, isobutil e benzil. In: XIII EVINCI, 2005. Livro de Resumos - 13.º EVINCI. Curitiba. p. 127.

• BRAGA, DANIEL ; LAST, D. ; SCHALK, F. ; LACKNER, G. . Towards the biosynthesis of the exotic cofactor F420 in Escherichia coli. 2017. (Apresentação de Trabalho/Conferência ou palestra).

• LIMA, D. B. ; LACKNER, G. ; HORN, U. ; HOFFMEISTER, D. . Investigation on polyketide biosynthesis in the white-rot fungus Fomitiporia mediterranea. 2014. (Apresentação de Trabalho/Simpósio).

• AGUSTINI, B. C. ; LIMA, D. B. ; BRAND, D. ; BONFIM, T. M. B. . Influência de etapas do processo de vinificação na acidez de vinhos elaborados com uvas Vitis labrusca. 2011. (Apresentação de Trabalho/Simpósio).

• SILVA, P. L. L. ; AGUSTINI, B. C. ; LIMA, D. B. ; LIMA JUNIOR, G. A. ; BRAND, D. ; BONFIM, T. M. B. . Production of biopigment (astaxanthin) by Phaffia rhodozyma for animal feed supplementation. 2010. (Apresentação de Trabalho/Congresso).

• BONFIM, T. M. B. ; BRAND, D. ; AGUSTINI, B. C. ; LIMA, D. B. . 9º Encontro de extensão e cultura da semana integrada de ensino, pesquisa e extensão. 2010. (Curso de curta duração ministrado/Extensão).

Projetos de pesquisa

• 2016 - Atual

  Genomics-driven discovery and synthetic biology of specialized metabolites from bacteria, Descrição: Bacteria are incredibly resourceful organisms. They produce natural products like enzymes, signaling molecules, cofactors or toxins. These molecules are extremely valuable for humans. Enzymes drive biotechnological applications in diagnostics or food processing. Cofactors serve as vitamins. Toxins can act as antibiotics or insecticides. Our aim is the discovery of novel biosynthetic routes useful for biotechnology, pharmacology or agriculture. Therefore, we investigate cryptic biosynthetic pathways, i.e. pathways with as-yet unknown products found encoded in microbial genomes. We combine functional genomics and comparative metabolomics to link biosynthetic gene clusters to their corresponding natural products. Functional genomics comprises techniques to perform targeted activation or inactivation of genes or to transfer and ?re-factor? whole gene clusters for expression in an ?optimized? host organism (Synthetic Biology). Comparative metabolomics refers to the systematic comparison of ?as many metabolites as possible? found in an organism. We employ high-resolution mass spectrometry and computational analyses to find novel specialized metabolites. In addition, we investigate the physiological role of the metabolites we discovered and study single biochemical steps in detail. Finally, we use synthetic biology to ?engineer? biosynthetic pathways: By channeling biomass into the biosynthetic route the yield of the natural product can be increased. Recombining pathways can lead to the production of novel metabolites with enhanced properties for human applications. Our current focus is on rare cofactors produced by bacteria of the genus Mycobacterium. Rare cofactors are involved in various intriguing physiological processes like growth on exotic carbon sources, defense against various stresses or antibiotic biosynthesis. Therefore, we make rare cofactors available for biotechnology and study their biosynthesis and physiology in the natural producers.. , Situação: Em andamento; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (2) / Doutorado: (2) . , Integrantes: Daniel Braga de Lima - Integrante / Gerald Lackner - Coordenador.

• 2013 - 2016

  Investigation of polyketide biosynthesis in agaricomycetes, Descrição: It is well established that small molecules mediate organismal interactions among eu- or prokaryotic microbes, or between them, e.g., through quorum sensing, inhibition, or inducing effects on metabolism. Recent progress in basidiomycete genomics unveiled a remarkable number of genes dedicated to small molecule biosynthesis, e.g. polyketides or small peptides. In stark contrast, little is known about the structures (let alone their bioactivities) of these secondary products and their effects on other microbial cells in subinhibitory concentrations, i.e., those titers that typically occur in shared habitats under natural environmental conditions. This ILRS project aims at research into the structural diversity of basidiomycete natural products and the effects they exert on other microbial cells. Specifically, parasitic species of the agaricomycotina (mushroom-type ?higher fungi?) will be characterized for their secondary metabolome, with an emphasis on polyketides. In a higher level, we expect to gather information about the role of these microorganisms in the microbial community and evidences about polyketide biosynthesis in mushroom-type fungi. We combine chemical analytics with genetic methods, to identify biosynthesis genes and connect them with fungal natural products. Their interactive relevance on other microbes and microbial communities will be investigated by specifically engineered reporter strains and activity-guided assay procedures.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Daniel Braga de Lima - Integrante / Uwe Horn - Integrante / Dirk Hoffmeister - Coordenador.

• 2009 - 2011

  Diferenciação genotípica e fenotípica de leveduras vínicas provenientes de Colombo, estado do Paraná, Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (2) / Mestrado acadêmico: (1) . , Integrantes: Daniel Braga de Lima - Integrante / Tânia Maria Bordin Bonfim - Coordenador.

Prêmios