Fernanda Sbaraini Gravina de Oliveira

Pós-doutorado

Formação complementar

Idiomas

Áreas de atuação

Organização de eventos

Participação em eventos

Produções bibliográficas

• DE OLIVEIRA, RAMATIS B ; PETIZ, LYVIA L ; Lim, Rebecca ; LIPSKI, JANUSZ ; GRAVINA, FERNANDA S ; BRICHTA, ALAN M ; CALLISTER, ROBERT J ; LEÃO, RICHARDSON N ; VAN HELDEN, DIRK F . Crosstalk between mitochondria, calcium channels and actin cytoskeleton modulates noradrenergic activity of locus coeruleus neurons. JOURNAL OF NEUROCHEMISTRY , v. 149, p. 471, 2019.

• HAHN, MARLA ; BAIERLE, MARÍLIA ; CHARÃO, MARIELE F. ; BUBOLS, GUILHERME B. ; Gravina, Fernanda S. ; ZIELINSKY, PAULO ; ARBO, MARCELO D. ; CRISTINA GARCIA, SOLANGE . Polyphenol-rich food general and on pregnancy effects: a review. Drug and Chemical Toxicology (New York, N.Y. 1978) , v. x, p. 1-7, 2016.

• GRAVINA, F. S. ; van Helden, Dirk F. ; KERR, KAREN P. ; de OLIVEIRA, R. B. ; JOBLING, PHILLIP . Phasic Contractions of the Mouse Vagina and Cervix at Different Phases of the Estrus Cycle and during Late Pregnancy. Plos One , v. 9, p. e111307, 2014.

• Rocha, Joao B. T. ; Saraiva, Rogerio A. ; Garcia, Solange C. ; Gravina, Fernanda S. ; Nogueira, Cristina W. . Aminolevulinate dehydratase (-ALA-D) as marker protein of intoxication with metals and other pro-oxidant situations. Toxicology Research , v. 1, p. 85-102, 2012.

• de Oliveira, Ramatis B. ; Gravina, Fernanda S. ; Lim, Rebecca ; Brichta, Alan M. ; Callister, Robert J. ; van Helden, Dirk F. . Heterogeneous Responses to Antioxidants in Noradrenergic Neurons of the Locus Coeruleus Indicate Differing Susceptibility to Free Radical Content. OXID MED CELL LONGEV , v. 2012, p. 1-10, 2012.

• Gravina, FS ; Jobling, P ; Kerr, KP ; Oliveira, RB de ; Parkington, HC ; Helden, DF van . Oxytocin depolarizes mitochondria in isolated myometrial cells. Experimental Physiology (Print) , v. 96, p. no-no, 2011.

• de Oliveira, Ramatis B. ; Gravina, Fernanda S. ; Lim, Rebecca ; Brichta, Alan M. ; Callister, Robert J. ; van Helden, Dirk F. . Developmental changes in pacemaker currents in mouse locus coeruleus neurons. Brain Research , v. 1425, p. 27-36, 2011.

• GRAVINA, F. S. ; Parkington, HC ; Kerr, KP ; De Oliveira, RB ; Jobling, P ; Coleman, HA ; Sandow, SL ; Davies, MM ; Imtiaz, MS ; Van Helden, DF . Role of mitochondria in contraction and pacemaking in the mouse uterus. British Journal of Pharmacology , p. no-no, 2010.

• de Oliveira, R.B. ; Howlett, M.C.H. ; Gravina, F.S. ; Imtiaz, M.S. ; Callister, R.J. ; Brichta, A.M. ; van Helden, D.F. . Pacemaker currents in mouse locus coeruleus neurons. NEUROSCIENCE , v. 170, p. 166-177, 2010.

• de Oliveira, Ramatis B. ; Graham, Brett ; Howlett, Marcus C.H. ; Gravina, Fernanda S. ; Oliveira, Max W.S. ; Imtiaz, Mohammad S. ; Callister, Robert J. ; Lim, Rebecca ; Brichta, Alan M. ; van Helden, Dirk F. . Ketamine anesthesia helps preserve neuronal viability. JOURNAL OF NEUROSCIENCE METHODS , v. 189, p. 230-232, 2010.

• GRAVINA, F. S. ; SILVEIRA, C. K. B. ; ASSIS, A. M. ; RIEGER, D. K. ; SOUZA, C. G. ; MULLER, A. P. ; FARINA, M. ; ROTTA, L. N. ; PERRY, M. L. S. . Experimental hypothyroidism inhibits -aminolevulinate dehydratase activity in neonatal rat blood and liver. EXPERIMENTAL BIOLOGY AND MEDICINE , v. 232, p. 1021-1026, 2007.

• MULLER, A. P. ; ROTTA, L. N. ; SCWEIGERT, I. ; LONDERO, L. ; GRAVINA, F. S. ; SILVEIRA, C. K. B. ; SOUZA, C. G. ; Battú, C. E. ; SOUZA, D. O. G. ; PERRY, M. L. S. . Effect of 2-deoxy-d-glucose on Aminoacids Metabolism in Rats Cerebral Cortex Slices. Neurochemical Research , v. 31, p. 417-422, 2005.

Projetos de pesquisa

• 2012 - 2012

  Efeitos deletérios dos polifenóis na constrição do ducto fetal e seus mecanismos, Descrição: A constrição do ducto arterioso fetal é uma desordem clínica que por muito tempo foi associada à ingestão materna de medicamentos anti-inflamatórios não-estereoidais no final da gestação. Esta constrição leva à um aumento na pressão do ventrículo direito do feto, com risco de desenvolver hipertensão arterial pulmonar neonatal, podendo evoluir para insuficiência cardíaca e até falência do feto. Recentes trabalhos na literatura vem apontando para uma nova fonte causadora da constrição do ducto fetal: os polifenóis. Estes compostos são encontrados em diversos alimentos, tais como: suco de uva, chocolate e alguns chás. Além de suas potentes propriedades antioxidantes, os polifenóis possuem atividade anti-inflamatórias, que por sua vez, poderiam ser as causadoras da constrição do ducto fetal. Este projeto investiga os possíveis efeitos deletérios dos polifenóis e seus mecanismos de contrição do ducto arterioso fetal, utilizando linhagem de células endoteliais de cordão umbilical humano (HUVEC). , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Mestrado acadêmico: (1) / Doutorado: (1) . , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / Garcia, Solange C. - Coordenador / rafael fracasso - Integrante / Fernanda Schwalm - Integrante / Mariele Charão - Integrante.

• 2010 - 2012

  Envelhecimento e morte da placenta humana, Situação: Desativado; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / Roger Smith - Coordenador.

• 2010 - 2011

  Ocitocina desporaliza mitocondria em células isoladas de miométrio, Descrição: Oxytocin is known to play important roles in uterine contractions, mediated at least in part by increasing intracellular Ca(2+) concentration ([Ca(2+)](i)), through enhancing extracellular Ca(2+) entry and Ca(2+) release from the sarcoplasmic reticulum, processes that are intimately linked with mitochondria. This study examined the effects of oxytocin on mitochondrial function. This was achieved by measuring the ratiometric JC-1 fluorescence signal in isolated myometrial cells, which provides a relative measure of the mitochondrial membrane potential ((m)), and also by loading the cells with Oregon Green BAPTA-AM to examine changes in [Ca(2+)](i). Oxytocin (1 nm) depolarized the (m) to 73.8 3.7% of the control value (P< 0.05; perfused for 11 min) and also caused a transient increase in [Ca(2+)](i). The depolarization of mitochondrial membrane potential was effectively reversed by 2-aminoethoxydiphenyl borate, nifedipine, Ca(2+)-free solution or oligomycin, with the ratiometric JC-1 fluorescence signal becoming no different from the control value in all cases (i.e.P> 0.05). The reduction in (m) is likely to occur at least in part through the oxytocin-induced increase in [Ca(2+)](i), causing enhanced mitochondrial uptake of Ca(2+) and resultant dissipation of the mitochondrial electrochemical gradient. ATP synthase is also stimulated, which would further contribute to a decrease in (m).. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / Parkington, HC - Integrante / Kerr, KP - Integrante / De Oliveira, RB - Integrante / Jobling, P - Integrante / van Helden, Dirk F. - Coordenador., Número de produções C, T & A: 1

• 2010 - 2011

  Sinalização de cálcio e potássio em cultura de células de miométrio humano, Descrição: Investigação de possíveis canais iônicos involvidos na iniciação do trabalho de parto. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / Roger Smith - Coordenador.

• 2009 - 2011

  Propriedades marca-passo e contráteis do trato reprodutor feminino, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Coordenador., Número de produções C, T & A: 2

• 2009 - 2011

  Efeito do canabinóide metanandamida nas contrações uterinas, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Coordenador., Número de produções C, T & A: 1

• 2008 - 2011

  Pacemaking neuronal, Situação: Concluído; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / de Oliveira, Ramatis B. - Coordenador., Número de produções C, T & A: 7

• 2007 - 2010

  Função da mitocondria nas contrações uterinas de camundongos, Descrição: Uterine spontaneous contraction and pacemaking are poorly understood. This study investigates the role of the mitochondrial Ca(2+) store in uterine activity. EXPERIMENTAL APPROACH: We investigated the effects of mitochondrial and sarco-endoplasmic reticulum (SER) inhibitors on contraction, membrane potential (Vm) and cytosolic Ca(2+) concentration ([Ca(2+) ](c) ) in longitudinal smooth muscle of the mouse uterus. KEY RESULTS: The mitochondrial agents rotenone, carbonylcyanide-3-chlorophenylhydrazone (CCCP), 7-chloro-5-(2-chlorophenyl)-1,5-dihydro-4,1-benzothiazepin-2(3H)-one (CGP37157) and kaempferol decreased the force of contractions. The ATP synthase inhibitor oligomycin had no significant effect. The effects of these agents were compared with those of SER inhibitors cyclopiazonic acid (CPA), 2-amino ethoxyphenylborate (2-APB) and caffeine. All agents, except CPA and oligomycin, decreased contractile force. CPA and CCCP transiently increased contraction frequency, which returned to control levels, whereas rotenone, CGP37157, kaempferol and 2-APB decreased frequency and caffeine had no significant effect. Application of the mitochondrial agents when CPA functionally inhibited stores did not change contraction frequency but, with the exception of kaempferol, decreased force. CCCP caused depolarization and maintained increase in [Ca(2+) ](c) or depolarization/transient hyperpolarization and transient increase in [Ca(2+) ](c) for oestrus and di-oestrus tissues respectively. Rotenone caused hyperpolarization and maintained increase in [Ca(2+) ](c) . CGP37157 and kaempferol caused hyperpolarization but no measurable change in [Ca(2+) ](c) . Application of a range of K(+) channel blockers indicated a role of Ca(2+) -activated K(+) (K(Ca) ) channels in the CCCP- and CGP37157-induced actions. CONCLUSIONS AND IMPLICATIONS: Mitochondria have a modulatory role on uterine contractions, with mitochondrial inhibition reducing contraction amplitude and pacemaker frequency by changes in Vm, [Ca(2+) ](c) and/or Ca(2+) influx.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (2) . , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / Parkington, HC - Integrante / Kerr, KP - Integrante / Jobling, P - Integrante / de Oliveira, R.B. - Integrante / van Helden, Dirk F. - Coordenador., Número de produções C, T & A: 4

• 2004 - 2007

  Efeito do hipotireoidismo sobre a atividade da enzima ALA-D, Descrição: The aim of this study was to investigate the potential relationship between hypothyroidism and delta-aminolevulinate dehydratase (delta-ALA-D) activity in rat blood and liver. Experimental hypothyroidism was induced in weanling rats by exposing their mothers to propylthiouracil (PTU) diluted in tap water (0.05% w/ v), ad libitum, during the lactational period (PTU group). Control (euthyroid) group included weanling rats whose mothers received just tap water, ad libitum, during the lactational period. Reverted-hypothyroid group (PTU + 3,3',5-triiodo-L-thyronine [T(3)]) included weanling rats whose mothers were exposed to PTU similarly to those in the hypothyroid group, but pups received daily subcutaneous injections of T(3) (20 microg/kg, from Postnatal Days 2-20). After the treatment, serum T(3) levels were drastically decreased (around 70%) in the PTU group, and this phenomenon was almost reverted by exogenous T(3). PTU decreased blood delta-ALA-D activity by 75%, and T(3) treatment prevented such phenomena. Erythrocytes and hemoglobin levels were increased by 10% in PTU-treated animals and higher increments (around 25%) were observed in these parameters when exogenous T(3) was coadministered. Dithiothreitol did not change blood delta-ALA-D activity of PTU-exposed animals when present in the reaction medium, suggesting no involvement of the enzyme's essential thiol groups in PTU-induced delta-ALA-D inhibition. PTU did not affect blood delta-ALA-D activity in vitro. These results are the first to show a correlation between hypothyroidism and decreased delta-ALA-D activity and point to this enzyme as a potential molecule involved with hypothyroidism-related hematological changes.. , Situação: Concluído; Natureza: Pesquisa. , Integrantes: Fernanda Sbaraini Gravina de Oliveira - Integrante / Liane N. Rotta - Integrante / Marcos L. S. Perry - Coordenador / Alexandre Pastoris Müller - Integrante / Clarice Kras Borges da Silveira - Integrante / Carolina Guerini Souza - Integrante / Adriano Martimbianco de Assis - Integrante / Debora K Rieger - Integrante / Marcelo Farina - Integrante.

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