Leandro Rodrigues Santiago

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Produções bibliográficas

• FAVREAU, EMELINE ; MARTÍNEZ-RUIZ, CARLOS ; RODRIGUES SANTIAGO, LEANDRO ; HAMMOND, ROBERT L ; WURM, YANNICK . Genes and genomic processes underpinning the social lives of ants. Current Opinion in Insect Science , v. 25, p. 83-90, 2018.

• FRANCISCO, FLÁVIO O. ; SANTIAGO, LEANDRO R. ; MIZUSAWA, YURI M. ; OLDROYD, BENJAMIN P. ; ARIAS, MARIA C. . Population structuring of the ubiquitous stingless bee Tetragonisca angustula in southern Brazil as revealed by microsatellite and mitochondrial markers. Insect Science , v. 24, p. 877-890, 2017.

• FRANCISCO, FLÁVIO O. ; SANTIAGO, LEANDRO R. ; MIZUSAWA, YURI M. ; OLDROYD, BENJAMIN P. ; ARIAS, MARIA C. . Genetic structure of island and mainland populations of a Neotropical bumble bee species. Journal of Insect Conservation , v. 20, p. 383-394, 2016.

• SANTIAGO, LEANDRO R. ; FRANCISCO, FLÁVIO O. ; JAFFÉ, RODOLFO ; ARIAS, MARIA C. . Genetic variability in captive populations of the stingless bee Tetragonisca angustula. Genetica ('s-Gravenhage) , v. no, p. no, 2016.

• VOLLET-NETO, AYRTON ; FERNANDO DOS SANTOS, CHARLES ; RODRIGUES SANTIAGO, LEANDRO ; DE ARAUJO ALVES, DENISE ; DE FIGUEIREDO, JÚLIA PINHEIRO ; NANZER, MARINO ; Arias, Maria Cristina ; IMPERATRIZ-FONSECA, VERA LUCIA . Diploid males of Scaptotrigona depilis are able to join reproductive aggregations (Apidae, Meliponini). Journal of Hymenoptera Research , v. 45, p. 125-130, 2015.

• JAFFÉ, RODOLFO ; PIOKER-HARA, FABIANA C. ; SANTOS, CHARLES F. ; SANTIAGO, LEANDRO R. ; ALVES, DENISE A. ; M. P. KLEINERT, ASTRID ; FRANCOY, TIAGO M. ; ARIAS, MARIA C. ; IMPERATRIZ-FONSECA, VERA L. . Monogamy in large bee societies: a stingless paradox. Naturwissenschaften , v. 101, p. 261-264, 2014.

• SANTIAGO, LEANDRO RODRIGUES ; PIOKER-HARA, FABIANA CURTOPASSI ; FRANCISCO, FLÁVIO DE OLIVEIRA ; BRITO, RUTE MAGALHÃES ; Gonçalves, Paulo Henrique Pereira ; DOMINGUES-YAMADA, ALAYNE MAGALHÃES TRINDADE ; Arias, Maria Cristina . Isolation and characterization of ten microsatellite loci in stingless bee Trigona spinipes (Apidae: Meliponini). Entomological Science (Tokyo) , v. 18, p. n/a-n/a, 2014.

• FRANCISCO, FLÁVIO DE OLIVEIRA ; SANTIAGO, LEANDRO RODRIGUES ; Arias, Maria Cristina . Molecular genetic diversity in populations of the stingless bee Plebeia remota: A case study. Genetics and Molecular Biology (Impresso) , v. 36, p. 118-123, 2013.

• BRITO, RUTE MAGALHÃES ; FRANCISCO, FLÁVIO DE OLIVEIRA ; FRANÇOSO, ELAINE ; SANTIAGO, LEANDRO RODRIGUES ; Arias, Maria Cristina . Very low mitochondrial variability in a stingless bee endemic to cerrado. Genetics and Molecular Biology (Impresso) , v. 36, p. 124-128, 2013.

• ARIAS, M. C. ATTEKE, CHRISTIANE AUGUSTO, S. C. BAILEY, J. BAZAGA, PILAR BEHEREGARAY, LUCIANO B. BENOIT, LAURE BLATRIX, RUMSAÏS BORN, CÉLINE BRITO, R. M. CHEN, HAI-KUI COVARRUBIAS, SARA DE VEGA, CLARA DJIÉTO-LORDON, CHAMPLAIN DUBOIS, MARIE-PIERRE FRANCISCO, F. O. GARCÍA, CRISTINA Gonçalves, P. H. P. GONZÁLEZ, CLEMENTINA GUTIÉRREZ-RODRÍGUEZ, CARLA HAMMER, MICHAEL P. HERRERA, CARLOS M. ITOH, H. KAMIMURA, S. KARAOGLU, H. , et al. KOJIMA, S. LI, SHOU-LI LING, HANNAH J. MATOS-MARAVÍ, PÁVEL F. MCKEY, DOYLE MEZUI-M'EKO, JUDICAËL ORNELAS, JUAN FRANCISCO PARK, R. F. POZO, MARÍA I. RAMULA, SATU RIGUEIRO, CRISTINA SANDOVAL-CASTILLO, JONATHAN SANTIAGO, L. R. SEINO, MIYUKI M. SONG, CHANG-BING TAKESHIMA, H. VASEMÄGI, ANTI WELLINGS, C. R. YAN, JI YU-ZHOU, DU ZHANG, CHANG-RONG ZHANG, TIAN-YUN ; Permanent Genetic Resources added to Molecular Ecology Resources Database 1 February 2013-31 March 2013. Molecular Ecology Resources (Print) , v. 13, p. 760-762, 2013.

• ALVES, A. N. ; FRANÇOSO, E. A. ; FRANCISCO, F. O. ; SANTIAGO, L. R. ; VILAS-BOAS, L. A. ; RUAS, C. F. ; ARIAS, M. C. ; SOFIA, S. H. . Isolation and characterisation of microsatellite loci in the wasp Mischocyttarus cassununga. Insectes Sociaux (Printed ed.) , v. 59, p. 565-570, 2012.

• Augusto, Solange Cristina ; Gonçalves, Paulo Henrique Pereira ; Francisco, Flávio ; SANTIAGO, LEANDRO RODRIGUES ; Françoso, Elaine Aparecida ; Suzuki, Karen Mayumi ; Sofia, Silvia Helena ; Simões, Zilá Luz Paulino ; Arias, Maria Cristina . Microsatellite loci for the carpenter bee Xylocopa frontalis (Apidae, Xylocopini). Conservation Genetics Resources (Print) , v. 000, p. 000-000, 2012.

• FRANCISCO, F. O. ; BRITO, R. M. ; SANTIAGO, L. R. ; Gonçalves, P. H. P. ; Pioker, F. C. ; Domingues-Yamada, A. M. T. ; ARIAS, M. C. . Isolation and characterization of 15 microsatellite loci in the stingless bee Plebeia remota (Apidae: Meliponini). Conservation Genetics Resources (Print) , v. 3, p. 417-419, 2011.

• SANTIAGO, LEANDRO RODRIGUES ; MUNIZ, THIAGO MAHLMANN VITORIANO LOPES ; OLIVEIRA, FAVÃZIA FREITAS DE ; BRITO, RUTE MAGALHÃES ; FRANCISCO, FLÃVIO DE OLIVEIRA . A fauna apÃ?Âcola do Parque Municipal da Cachoeirinha (IporÃ?Â, GO). Biota Neotropica , v. 9, p. 393-397, 2009.

• SANTIAGO, LEANDRO RODRIGUES ; FRANCISCO, FLÁVIO DE OLIVEIRA ; ARIAS, MARIA C. . Genetic variability of captive populations of Tetragonisca angustula, a highly eusocial stingless bee.. In: 48th Population Genetics Group, 2015, Sheffield. POPGROUP48 Sheffield, 2015. p. 45-45.

• FRANCISCO, FLÁVIO O. ; SANTIAGO, LEANDRO RODRIGUES ; MIZUSAWA, Y. ; OLDROYD, BENJAMIN P. ; ARIAS, MARIA C. . Genetic structure of social bees in Neotropic continental islands. In: 17th Congress of the International Union for the Study of Social Insects (IUSSI),, 2014, Cairns. International Union for the Study of Social Insects International Congress Abstracts, 2014. p. 146-146.

• SANTIAGO, LEANDRO RODRIGUES ; FRANCISCO, FLÁVIO O. ; ARIAS, MARIA C. . Genetic variability of captive breeding populations of Tetragonisca angustula.. In: 17th Congress of the International Union for the Study of Social Insects (IUSSI),, 2014, Cairns. International Union for the Study of Social Insects International Congress Abstracts, 2014. p. 283-283.

• SANTIAGO, L. R. ; FRANCISCO, F. O. ; ARIAS, M. C. . HIGH NUCLEAR GENETIC VARIABILITY IN Tetragonisca angustula FROM MELIPONARIES. In: X Encontro Sobre Abelhas, 2012, Ribeirão Preto. ANAIS DO X ENCONTRO SOBRE ABELHAS RIBEIRÃO PRETO. Ribeirão Preto: FUNPEC-Editora, 2012. v. 10. p. 444.

• FRANCISCO, F. O. ; SANTIAGO, L. R. ; ARIAS, M. C. . NATURAL HYBRIDIZATION BETWEEN Tetragonisca angustula AND T. fiebrigi. In: X Encontro Sobre Abelhas, 2012, Ribeirão Preto. Anais do X Encontro Sobre Abelhas Ribeirão Preto. Ribeirão Preto: FUNPEC-Editora, 2012. v. 10. p. 269.

• SANTIAGO, L. R. ; AYELE, A. G. ; TILAHUN, M. ; ABREHA, Z. ; DRUMOND, P. M. ; ARIAS, M. C. . MITOCHONDRIAL AND MICROSATELLITES POLYMORPHISM IN ETHIOPIAN HONEYBEE. In: X Encontro Sobre Abelhas, 2012, Ribeirão Preto. Anais do X Encontro Sobre Abelhas Ribeirão Preto. Ribeirão Preto: FUNPEC-Editora, 2012. v. 10. p. 445.

• SANTIAGO, L. R. ; ARIAS, M. C. . Inbreeding in Tetragonisca angustula (Hymenoptera, Apidae, Meliponini) from meliponaries?. In: 57 Congresso Brasileiro de Genética, 2011, Águas de Lindóia. Resumos do 57 Congresso Brasileiro de Genética, 2011. p. 106.

• FRANCISCO, F. O. ; SANTIAGO, L. R. ; OLDROYD, B. P. ; ARIAS, M. C. . Genetic structure of island and mainland populations of bees from Atlantic forest. In: 57 Congresso Brasileiro de Genética, 2011, Águas de Lindóia. Resumos do 57 Congresso Brasileiro de Genética, 2011. p. 113.

• BRITO, R. M. ; FRANCISCO, F. O. ; Francoso, E.A. ; SANTIAGO, L. R. ; ARIAS, M. C. . Evidence of bottleneck in Partamona mulata (Hymenoptera, Apidae, Meliponini) revealed by low mitochondrial polymorphism. In: 57 Congresso Brasileiro de Genética, 2011, Águas de Lindóia. Resumos do 57 Congresso Brasileiro de Genética, 2011. p. 8.

• SANTIAGO, L. R. ; FRANCISCO, F. O. ; ARIAS, M. C. . ANÁLISE DA VARIABILIDADE GENÉTICA DE AMOSTRAS DE Tetragonisca angustula e Tetragonisca fiebrigi DE MELIPONÁRIOS. In: IX Enconttro sobre abelhas, 2010, Ribeirão Preto. Anais do IX Encontro sobre Abelhas. Ribeirão Preto: FUNPEC-Editora, 2010. v. 9. p. 472.

• Gonçalves, P. H. P. ; SANTIAGO, L. R. ; Barroso, G.V. ; Francoso, E.A. ; FRANCISCO, F. O. ; ARIAS, M. C. . GENETIC STRUCTURE OF A DRONE CONGREGATION AREA OF TETRAGONISCA ANGUSTULA (APIDAE, MELIPONINI) IN SÃO PAULO CITY, BRAZIL. In: XVI IUSSI 2010, 2010, Copenhague. Abstracts XVI IUSSI 2010, 2010. p. 152.

• SANTIAGO, L. R. ; BRITO, R. M. ; FRANCISCO, F. O. ; ARIAS, M. C. . Análise da diversidade de espécies e caracterização do DNA mitocondrial de abelhas do Parque Ecológico da Cachoeirinha (Iporá, GO). In: Anais do 51 Congresso Brasileiro de Genética, 2005, Águas de Lindóia. 51 Congresso Brasileiro de Genética, 2005.

Projetos de pesquisa

• 2014 - 2019

  Social insects to understand the mechanisms for variation in lifespan: gene expression differences between long-lived and short-lived colony members, Descrição: A fundamental unsolved question in biology is understanding the molecular-genetic mechanisms responsible for senescence and longevity. Some theories have been postulated to explain the process of ageing. Three relevant theories are centred on oxidative stress, DNA damage, and Disposable soma. Work on model organisms have linked the activity of several genes and pathways, specifically the insulin/IGF-1 signalling pathway, to their lifespans. However, it is unclear to which extent aging mechanisms in such short-lived organisms also apply to long-lived organisms. Here, I take advantage of intra-specific variation in lifespan in social insects. They naturally feature long maximal lifespans and tremendous intra-specific differences in expected longevity. Moreover, the intra-specific differences are due to gene regulatory mechanisms and are not confounded by genetic background. In order to test to which extent the different theories of ageing, and the insulin/IGF-1 signalling pathway, are validated by patterns seen in social insects, I used available RNA-seq data to perform analyses of differential expression independently in Bombus terrestris, Linepithema humile, Monomorium pharaonis, Solenopsis invicta, Vollenhovia emeryi, Wasmannia auropunctata and Zootermopsis nevadensis. I firstly found higher expression in workers than in queens in B. terrestris and over representation of genes associated with oxidative stress, DNA repair and insulin/IGF-1 signalling pathways. In addition, some genes showed the similar gene expression profile as described for model organisms in the GenAge database. Secondly, I found a higher activity of Piwi-Interacting RNA genes in queens in comparison to workers in W. auropunctata, which is in line with the disposable soma theory. Finally, I identified genes that are similarly associated to lifespan across seven species of social insects. The results of this analysis suggest that selection for longevity has driven changes in the use of multiple mechanisms to regulate lifespan in social insects. Additionally, they show that related or identical genes are likely to have been recruited for lifespan evolution in the ancestors of the seven studied species, thus showing a general pattern of regulation of longevity shared by social insects.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Doutorado: (1) . , Integrantes: Leandro Rodrigues Santiago - Integrante / YANNICK WURM - Coordenador., Financiador(es): Conselho Nacional de Desenvolvimento Científico e Tecnológico - Bolsa.

• 2012 - 2012

  Technical training on collecting bees and molecular analysis., Projeto certificado pelo(a) coordenador(a) Maria Cristina Arias em 21/12/2012., Descrição: This project aims to provide to the student the opportunity of gathering experience on collecting bees and analysing data by helping postgraduate student to develop their research. The student will be responsible for collecting Tetragonisca angustula, Bombus morio and Bombus pauloensis for two PhD projects. He will also be involved in the data analysis and laboratory experiments related to both projects. Thereat, the student will obtain training on population genetics data analysis and laboratory techniques, such as cloning, bacterial transformation, sequencing, DNA and RNA extraction, etc.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Mestrado acadêmico: (1) . , Integrantes: Leandro Rodrigues Santiago - Integrante / Maria Cristina Arias - Coordenador., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Bolsa., Número de produções C, T & A: 1

• 2010 - 2013

  Genetic variability of Tetragonisca angustula (Hymenoptera, Apidae, Meliponini) from meliponaries., Projeto certificado pelo(a) coordenador(a) Maria Cristina Arias em 19/01/2013., Descrição: Low genetic variability has normally been considered a consequence of animal husbandry as well as a major contributing factor to bee decline. Here we performed molecular analysis of captive populations of the stingless bee Tetragonisca angustula, one of the most commonly kept species across South America. We sampled 430 individuals, one per nest, from nine distinct meliponaries (same as apiaries, but for stingless bees) in Sao Paulo and Parana states, Brazil. As positive control for genetic variability, we reanalyzed data from wild samples reported in previous studies. Employing microsatellite markers, we verified a similar genetic variability between wild and captive populations. Nonetheless, genetic variability differences at mitochondrial DNA were remarkable between populations. Gene flow by males, transport of nests and division of colonies are the most probable explanations for the observed pattern of genetic structure. We conclude that increasing the number of colonies kept through nest divisions does not negatively affect nuclear genetic variability, which seems to be maintained by local male dispersal and human-mediated nest transport.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Mestrado acadêmico: (1) . , Integrantes: Leandro Rodrigues Santiago - Integrante / Maria Cristina Arias - Coordenador., Financiador(es): Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Bolsa.

• 2008 - 2010

  Technical training on collecting bees and molecular analysis., Descrição: Natural stingless bees populations are poorly known, which means that the impact of habitat fragmentation on those population cannot be yet evaluated properly. Recent techniques on molecular genetics allows us to get a proper estimative of the genetic variability of existing population and how large they should be to be genetically healthy. The comprehension of those processes is crucial for every effort toward conservation. The laboratory of genetics and evolution of bees, which uses molecular markers to better understand the systematics and population dynamics of stingless bees, have initiated research on this direction. In University of Sao Paulo campus in Ribeirao Preto city, and surrounding natural environment, about 1025 were located and are constantly monitored. This project aims to perform the same type work in the University of Sao Paulo, Sao Paulo campus, which will represent a small population in anthropic area. United, both data will allow us to perform population comparisons, which will improve our understanding on the biology of stingless bees. We will get both information about gene flow, genetic variability in different environmental and captive conditions and possible evidence of speciation processes. The application of the acquired knowledge will increase our understanding about stingless bee beekeeping and how to preserve those species properly.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (1) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Leandro Rodrigues Santiago - Integrante / Vera Lúcia Imperatriz Fonseca - Coordenador., Financiador(es): Fundação de Amparo à Pesquisa do Estado de São Paulo - Bolsa.

• 2004 - 2005

  Survey of the species diversity and characterisation of the mitochondrial DNA of bees from Parque Ecológico da Cachoeirinha, Goias state, Brazil., Descrição: Bees are dependent on flower nectar and pollen (main sources of protein and energy, respectively) and have an important economic and ecological role as pollinators, representing about 40 to 90% of phanerogams pollinators in several ecosystems. This work presents a description of bee species from Parque Municipal da Cachoeirinha in Iporá, Goiás state, Brazil. In order to have an overall idea of the genetic diversity of those bees, we also performed a molecular analysis through PCR+RFLP of the mitochondrial DNA in six species. A total of 58 hours of capturing were performed on November and December 2004 and on March and May 2005. Nine hundred thirty-three individuals were collected, distributed in one family, 16 tribes, 33 genera, and 62 species. Meliponini tribe presented the highest number of individuals and species. Most abundant species were Trigona chanchamayoensis (16.51%), Tetragona clavipes (14.79%) and Apis mellifera and Trigona recursa (12.33%). By using three restriction enzymes on mitochondrial DNA fragments amplified via PCR, 5 distinct haplotypes were found among the six species, indicating moderate genetic variability. This work presents the first occurrence in Goiás state for six genera and 12 species.. , Situação: Concluído; Natureza: Pesquisa. , Alunos envolvidos: Graduação: (2) / Especialização: (0) / Mestrado acadêmico: (0) / Mestrado profissional: (0) / Doutorado: (0) . , Integrantes: Leandro Rodrigues Santiago - Integrante / Flávio de Oliveira Francisco - Coordenador / Rute Magalhães Brito - Integrante / Favízia Freitas de Oliveira - Integrante / MUNIZ, THIAGO MAHLMANN VITORIANO LOPES - Integrante., Financiador(es): UEG/CNPq - Bolsa., Número de produções C, T & A: 1